Cloning and Bioinformatics Analysis of ORF51 Gene of Anguillae Herpesvirus FJ Strain

Anguillae herpesvirus Fujian strain(AngHV-FJ)was first isolated from China mainland by our lab.To study the structure and function of AngHV-FJ ORF51,AngHV-FJ was propagated,and the ORF51 gene was amplified by PCR from the extracted virus genomic DNA,then ORF51was cloned into the vector pMD19-T for sequencing.The nucleotide and protein sequence of ORF51 was analyzed by bioinformatics softwares.The ORF51 gene is 720bp in length,encoding 239amino acids,with 100%identity with AngHV-1ORF51gene.The protein has 26107.1Da molecular weight,7.66 isoelectric point,hydrophobic and alkaline,four transmembrane regions with high antigenicity.Structure analysis indicated that,the protein has 1N-glycosylation sites,1O-glycosylation sites and 10 phosphorylation sites.Subcellular localization prediction showed that the protein is mainly localized in the endoplasmic reticulum.The study provided a reference for the future ORF51 prokaryotic or eukaryotic expression,and established the foundation for the Anguillae herpesvirus research on genetic background information,pathogenesis,molecular epidemiology and genetic engineering vaccine development.