Identification and Gene Mapping of a Lesion Mimic and Senescence Mutant lms1 in Rice

A lesion mimic and senescence mutant,designated as lms1,was isolated from the tissue culture progeny of indica rice restorer line Minghui-86.lms1plants started to appear small brown spots on the leaves in elongation stage,and the spots gradually spreaded to whole leaves and sheaths.With the growth of the plants to heading stage,lms1displayed a senescent phenotype,whereas leaves,stems,and panicles dried obviously,resulting in whole plants′death.RT-PCR analysis revealed that the expression of pathogenesis-related genes PBZ1and PAL1 was induced in lms1leaf tissues.Genetic analysis suggested that the loci for the mutant phenotype was controlled by a single recessive gene.F2and F3 populations derived from a cross between 9311and lms1 were used for gene mapping,and the lms1locus was mapped close to the terminal region of the long arm of chromosome 11.