ELISA Identification and Development of RT-PCR Detection of Sweet Potato Feathery Mottle Virus

XU Yong-qing; LI Hua-wei; LIU Zhong-hua; QIU Yong-xiang; LUO Wen-bin; JI Rong-chang; TANG Hao; QIU Si-xin; YU Hua

doi:10.19303/j.issn.1008-0384.2013.12.016

Volume 28 Issue 12

Dec. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(12): 1267-1272

XU Yong-qing, LI Hua-wei, LIU Zhong-hua, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, QIU Si-xin, YU Hua. ELISA Identification and Development of RT-PCR Detection of Sweet Potato Feathery Mottle Virus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(12): 1267-1272. doi: 10.19303/j.issn.1008-0384.2013.12.016

Citation:

XU Yong-qing, LI Hua-wei, LIU Zhong-hua, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, QIU Si-xin, YU Hua. ELISA Identification and Development of RT-PCR Detection of Sweet Potato Feathery Mottle Virus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(12): 1267-1272. doi: 10.19303/j.issn.1008-0384.2013.12.016

Citation:

XU Yong-qing, LI Hua-wei, LIU Zhong-hua, QIU Yong-xiang, LUO Wen-bin, JI Rong-chang, TANG Hao, QIU Si-xin, YU Hua. ELISA Identification and Development of RT-PCR Detection of Sweet Potato Feathery Mottle Virus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(12): 1267-1272. doi: 10.19303/j.issn.1008-0384.2013.12.016

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ELISA Identification and Development of RT-PCR Detection of Sweet Potato Feathery Mottle Virus

doi: 10.19303/j.issn.1008-0384.2013.12.016

1. Institute of Crop Sciences,Fujian Academy of Agricultural Sciences;
2. Central Laboratory,Fujian Academy of Agricultural Sciences

Received Date: 2013-10-21
Publish Date: 2013-12-18

Abstract

Abstract

51samples of sweet potato from Fujian province were detected by the double antibody enzyme-linked immunosorbent method (DAS-ELISA) .The results showed that 24samples were SPFMV-positive, the positive rate were 47.06%;the highest positive rate was 71.40%, detected in the samples from Quanzhou, and the next were 50.00%, detected in samples from Nanan and Longyan.A pair of primer was designed and synthesized according to the published nucleotide sequence of coat protein gene of SPFMV, and a specific RT-PCR detection procedure was established.The amplified SPFMV-specific fragment in PCR contained 441bps, and showed 92%-97%, homology with the SPFMV reference sequence.
- sweet potato,
- virus,
- sweet potato feathery mottle virus,
- ELISA,
- RT-PCR

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References(14)

References

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