Study on the Ultrasonic Extraction Process of the Total Flavonoids in Pigeon Pea Leaves

ZHENG Fei-yan; LI Ai-ping; XU Xiao-yu; LAI You-hui; ZHENG Kai-bin

doi:10.19303/j.issn.1008-0384.2013.11.015

Volume 28 Issue 11

Nov. 2013

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(11): 1149-1153

ZHENG Fei-yan, LI Ai-ping, XU Xiao-yu, LAI You-hui, ZHENG Kai-bin. Study on the Ultrasonic Extraction Process of the Total Flavonoids in Pigeon Pea Leaves[J]. Fujian Journal of Agricultural Sciences, 2013, 28(11): 1149-1153. doi: 10.19303/j.issn.1008-0384.2013.11.015

Citation:

ZHENG Fei-yan, LI Ai-ping, XU Xiao-yu, LAI You-hui, ZHENG Kai-bin. Study on the Ultrasonic Extraction Process of the Total Flavonoids in Pigeon Pea Leaves[J]. Fujian Journal of Agricultural Sciences, 2013, 28(11): 1149-1153. doi: 10.19303/j.issn.1008-0384.2013.11.015

Citation:

PDF( 1005 KB)

Study on the Ultrasonic Extraction Process of the Total Flavonoids in Pigeon Pea Leaves

doi: 10.19303/j.issn.1008-0384.2013.11.015

1. College of Food Science,Fujian Agriculture and Forestry University;
2. Fujian Senhui Farming and Stock Breeding Development Co.,LTD;
3. Corp Research Institute,Fujian Academy of Agricultural Sciences;
4. Sugarcane Research Institute,Fujian Academy of Agricultural Sciences

Received Date: 2013-07-30
Publish Date: 2013-11-18

Abstract

Abstract

Extracting total flavonoids in pigeon pea leaves by ultrasonic method.Analysis the factors which ultrasonic extraction time, the concentration of ethanol and the solid-liquid ratio condition and L9 (34) orthogonal, to find the optimal conditions of extraction of total flovonoids in pigeon pea leaves.From the orthogonal results of ultrasonic extraction, the optimal conditions of extraction of total flavonoids in pigeon pea leaves are as following: 20min ultrasonic extraction 70%ethanol, 1∶30solid-liquid ratio.With above extraction condition the yield of total flavonoids is 3.65%.The average recovery of the detection method is 99.01%-102.72%, the method has excellent stability and repeatability.
- pigeon pea leaves,
- total flavonoids,
- ultrasonic extraction,
- extraction process

FullText(HTML)

References(16)

References

[1]	李正红, 周朝鸿, 谷勇, 等.中国木豆研究利用现状及开发前景[J].林业科学研究, 2001, 14 (6) :674-681.
[2]	刘秀贤, 李正红, 张建云, 等.不同种皮色木豆蛋白质淀粉含量的研究[J].林产化学与工业, 2002, 22 (3) :49-53.
[3]	刘秀贤, 李正红, 邓疆, 等.不同品种及单株木豆叶蛋白质含量的研究[J].生物质化学工程, 2006, 40 (4) :19-21.
[4]	孙绍美, 宋玉梅, 刘俭, 等.木豆素制剂药理作用研究[J].中草药, 1995, 26 (3) :147-148.
[5]	田润, 尹爱武, 王盼.木豆叶化学成分研究[J].湖南科技学院学报, 2012, 33 (4) :68-69.
[6]	向锦, 庞雯, 王建红.木豆在中国的应用前景[J].四川草原, 2003, (4) :38-40.
[7]	钟小荣.木豆的利用价值[J].中药研究与信息, 2001, 3 (8) :47-47.
[8]	HABIB M A, ANISUZZAMAN A S M, KHAN M R I, et al.Chemical and pharmacological characterization of hypolipidemic compound fromCajanus Cajan[J].Bangladesh Journal of Pharmacology, 2010, 5 (1) :34-38.
[9]	YUAN-GNAG ZU, YU-JIE FU, WEI LIU, et al.Simultaneous determination of four flavonoids in pigeonpea[Cajanus cajan (L.) Millsp.]leaves using RP-LC-DAD[J].Chromatographia, 2006, 63 (9) :499-505.
[10]	FU Y, ZU Y, LIU W, et al.Preparative separation of vitexin and isovitexin from pigeonpea extracts with macroporous resins[J].Journal of chromatography, 2007, 1139 (2) :206-213.
[11]	袁浩, 赖小平, 何伟, 等.通络生骨胶囊的研制与开发[J].中国医药杂志, 2004, (21) :88-90.
[12]	袁捷.木豆叶及其提取物用于制备骨性关节炎药物的用途[P].CN:200510117299.X.
[13]	AMARTEIFIO J O, MUNTHALI D C, KARIKARI S K, et al.The composition of pigeon peas (Cajanus cajan (L.Millsp.) grown in Botswana[J].Plant Foods for Human Nutrition (Formerly Qualitas Plantarum) , 2002, 57 (2) :173-177.
[14]	赵恩泽, 金时, 魏作富, 等.木豆叶总黄酮测定方法的比较研究[J].植物研究, 2011, 31 (4) :499-502.
[15]	刘中秋, 周华, 林励, 等.生脉成骨片中木豆叶提取工艺研究[J].中成药, 1998, 20 (3) :7-9.
[16]	侯春莲.木豆叶中牡荆苷与异牡荆苷提取纯化工艺及其抗菌活性研究[D].哈尔滨:东北林业大学, 2006.