Induction,Subculture and Maintenance of Fuzhou Jasmine,Jasminum sambac (Linn.) Aiton

GAN Huang-can; LAI Cheng-chun; ZHU Yu-jing; CHEN Wen-hui; LI Yan-hui; LIU Bo

doi:10.19303/j.issn.1008-0384.2013.10.009

Volume 28 Issue 10

Oct. 2013

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GAN Huang-can, LAI Cheng-chun, ZHU Yu-jing, CHEN Wen-hui, LI Yan-hui, LIU Bo. Induction,Subculture and Maintenance of Fuzhou Jasmine,Jasminum sambac (Linn.) Aiton[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 976-980. doi: 10.19303/j.issn.1008-0384.2013.10.009

Citation:

GAN Huang-can, LAI Cheng-chun, ZHU Yu-jing, CHEN Wen-hui, LI Yan-hui, LIU Bo. Induction,Subculture and Maintenance of Fuzhou Jasmine,Jasminum sambac (Linn.) Aiton[J]. Fujian Journal of Agricultural Sciences, 2013, 28(10): 976-980. doi: 10.19303/j.issn.1008-0384.2013.10.009

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Induction,Subculture and Maintenance of Fuzhou Jasmine,Jasminum sambac (Linn.) Aiton

doi: 10.19303/j.issn.1008-0384.2013.10.009

1. College of Biological Sciences and Technology,Fuzhou University;
2. Institute of Agricultural Engineering and Technology,Fujian Academy of Agricultural Sciences;
3. Agricultural Bioresource Research Institute,Fujian Academy of Agricultural Sciences;
4. Agricultural Bureau

Received Date: 2013-05-12
Publish Date: 2013-10-18

Abstract

Abstract

Plantlets of Fuzhou jasmine, Jasminum sambac (Linn.) Aiton, were obtained by using the stems and floral organ of the plant as explants.The callus induction, subculture and long-term maintenance of the plantlets were investigated on media with varied concentrations of phytohormone.The results indicated that the optimal light density for the culture was 1 500-2 000lx, and that 6different types of callus could be formed by varying the phytohormone concentration.The medium consisting of MS, 2.0mg BA/L, 0.2mg NAA·L-1 and 30g sucrose ·L-1 yielded a 100%induction rate, and at the same time, was the best for the callus subculture multiplication, as well as the growth of the induced plants.
- jasmine[Jasminum sambac (Linn.) Aiton],
- explant,
- callus,
- induction,
- subculture

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References(19)

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