Orthogonal Optimization for Propagation of Clustered OncidiumBuds

YE Xiu-xian; HUANG Min-ling; LUO Yuan-hua; WU Jian-she; LIN Bing

doi:10.19303/j.issn.1008-0384.2013.09.013

Volume 28 Issue 9

Sep. 2013

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YE Xiu-xian, HUANG Min-ling, LUO Yuan-hua, WU Jian-she, LIN Bing. Orthogonal Optimization for Propagation of Clustered OncidiumBuds[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 897-901. doi: 10.19303/j.issn.1008-0384.2013.09.013

Citation:

YE Xiu-xian, HUANG Min-ling, LUO Yuan-hua, WU Jian-she, LIN Bing. Orthogonal Optimization for Propagation of Clustered OncidiumBuds[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 897-901. doi: 10.19303/j.issn.1008-0384.2013.09.013

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Orthogonal Optimization for Propagation of Clustered OncidiumBuds

doi: 10.19303/j.issn.1008-0384.2013.09.013

YE Xiu-xian^1,2,3,
HUANG Min-ling^1,2,3,
LUO Yuan-hua^1,2,3,
WU Jian-she^1,2,3,
LIN Bing^1,2,3

1. Institute of Crop Sciences,Fujian Academy of Agricultural Sciences;
2. Flowers Research Center,Fujian Academy of Agricultural Sciences;
3. Fujian Engineering Research Center for Characteristic Floriculture

Received Date: 2013-06-21
Publish Date: 2013-09-18

Abstract

Abstract

Peduncle axillary buds of the Little Cherry, Oncidium, were induced as explants.The orthogonal design was used to evaluate the effects of the culture medium containing inorganic substances, 6-BA, NAA and LH on the propagation of Oncidiumbuds.The result showed that 6-BA, the base medium, NAA and LH affected significantly on the bud multiplication.The optimal medium was the Improved Formulation No.1, which consisted of the base medium, 6-BA 3.0mg·L-1, NAA 0.1mg·L-1 and LH0.5g·L-1, and the average propagation coefficient was 6.53 in 45days.The breeding efficiency was effectively improved.The result appeared to provide a basis for the commercialization of the seedling propagation.
- Oncidium,
- bud clumps,
- orthogonal design,
- tissue culture

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References(13)

References

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