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1. Rice Research Institute,Fujian Academy of Agricultural Sciences;
2. Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture,P.R China/Fuzhou branch,National Rice Improvement Center of China/FujianEngineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding;
3. Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences &Technology;
4. National Rice Engineering Laboratory of China;
5. South China Bases of National Key Laboratory of Hybrid Rice for China
For the genetic analysis of minor effect restoring gene in rice, the CMS lines of Zhenshan 97A, Longtepu A and Jinfu 1A with different fertile levels were used as materials to investigate three sterility indexes, bagged seed setting rate, dark pollen rate by I2-IK dyed and rate of florets with fertile anthers.The results showed that the appearance of abnormal plants in CMS-WA was due to partial restoration of self-fertility few floras caused by minor effect restoring gene.Adopted the method of genetic improvement could eliminate part of minor effect restoring gene to get purification and elimination, but not completely ruled out.This index of rate of florets with fertile anthers could be use as a suitable tool to study heredity laws of minor effect restoring genes and its further molecular mechanism.