Cloning and Expression of Key Enzyme Genes Involved in Phalaenopsis Anthocyanins Synthesis

ZHONG Huai-qin; HUANG Min-ling; WU Jian-she; FAN Rong-hui

doi:10.19303/j.issn.1008-0384.2013.09.004

Volume 28 Issue 9

Sep. 2013

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ZHONG Huai-qin, HUANG Min-ling, WU Jian-she, FAN Rong-hui. Cloning and Expression of Key Enzyme Genes Involved in Phalaenopsis Anthocyanins Synthesis[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 854-858. doi: 10.19303/j.issn.1008-0384.2013.09.004

Citation:

ZHONG Huai-qin, HUANG Min-ling, WU Jian-she, FAN Rong-hui. Cloning and Expression of Key Enzyme Genes Involved in Phalaenopsis Anthocyanins Synthesis[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 854-858. doi: 10.19303/j.issn.1008-0384.2013.09.004

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Cloning and Expression of Key Enzyme Genes Involved in Phalaenopsis Anthocyanins Synthesis

doi: 10.19303/j.issn.1008-0384.2013.09.004

1. Institute of Crop Sciences,Fujian Academy of Agricultural Sciences;
2. Flowers Research Center,Fujian Academy of Agricultural Sciences;
3. Fujian Engineering Research Center of Characteristic Floriculture

Received Date: 2013-06-25
Publish Date: 2013-09-18

Abstract

Abstract

Four key enzyme genes (CHS, DFR, F3′5′H, ANS) involved in anthocyanins synthesis were cloned from the flower petals of Phalaenopsis with homology sequence cloning.The length of the sequence was 302bp, 275bp, 285bp and285bp, respectively.Sequence analysis showed that these protein shared high similarity to other proteins from Dendrobium, Cymbidiumet al, ranging from 81%-95%, 67%-91%, 68%-92%and 83%-89%, respectively.The results of phylogenetic analysis were in agreement with those described in plant taxonomy.Resultsof Real-time fluorescence quantitative PCR analysis showed that the relative expression level of CHSand F3′5′H gene was the highest in the flower large bud and early flowering stages, and then decreased in blooming.The expression level of CHSand F3′5′H gene in petals and lip was greater than in sepals, the trace expression revealed in Leaves and roots.
- Phalaenopsis,
- anthocyanin,
- gene cloning,
- gene expression

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References(13)

References

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