Transduction of Rice Blast Resistance Gene Pi-9 Using Sanming Dominant Genic Male Sterile as the Vector

TANG Jiang-xia; LIN Cheng-bao; HUANG Xian-bo; DENG Ze-qin; LIN Mei-juan; LIANG Shui-jin

doi:10.19303/j.issn.1008-0384.2013.09.003

Volume 28 Issue 9

Sep. 2013

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TANG Jiang-xia, LIN Cheng-bao, HUANG Xian-bo, DENG Ze-qin, LIN Mei-juan, LIANG Shui-jin. Transduction of Rice Blast Resistance Gene Pi-9 Using Sanming Dominant Genic Male Sterile as the Vector[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 849-853. doi: 10.19303/j.issn.1008-0384.2013.09.003

Citation:

TANG Jiang-xia, LIN Cheng-bao, HUANG Xian-bo, DENG Ze-qin, LIN Mei-juan, LIANG Shui-jin. Transduction of Rice Blast Resistance Gene Pi-9 Using Sanming Dominant Genic Male Sterile as the Vector[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 849-853. doi: 10.19303/j.issn.1008-0384.2013.09.003

Citation:

TANG Jiang-xia, LIN Cheng-bao, HUANG Xian-bo, DENG Ze-qin, LIN Mei-juan, LIANG Shui-jin. Transduction of Rice Blast Resistance Gene Pi-9 Using Sanming Dominant Genic Male Sterile as the Vector[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 849-853. doi: 10.19303/j.issn.1008-0384.2013.09.003

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Transduction of Rice Blast Resistance Gene Pi-9 Using Sanming Dominant Genic Male Sterile as the Vector

doi: 10.19303/j.issn.1008-0384.2013.09.003

Sanming Academy of Agricultural Sciences

Received Date: 2013-08-25
Publish Date: 2013-09-18

Abstract

Abstract

Transduction of rice blast resistance gene Pi-9 for rice was studied with Sanming dominant genic male sterile rice as the vector and the rice blast resistance 75-1-127 with Pi-9 gene as the donor.Pi-9 gene was introduced into the rice restore Shuangkangmingzhan by successive backcrosses with supporting molecular markers.The results indicated that the pure lines of HP304 with Pi-9 gene similar to Shuangkangmingzhan had been selected from the fertile plants of BC4F3.The basic genetic background of HP304 had been proved to be the same to that of Shuangkangmingzhan. The main agronomic traits of HP304 had no significant difference with Shuangkangmingzhan.And the resistance identification in field showed that the resistant level of HP304 was significantly higher than that of Shuangkangmingzhan.The above demonstrated that it was available to transfer Pi-9 gene into rice using Sanming dominant geneic male sterile as the vector.
- rice,
- sanming dominant genic male sterile,
- blast,
- Pi-9

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References(19)

References

[1]	吴建利, 庄杰云, 李德葆, 等.水稻对稻瘟病抗性的分子生物学研究进展[J].中国水稻科学, 1999, 13 (2) :123-128.
[2]	孙国昌, 杜新法, 陶荣祥, 等.水稻稻瘟病防治策略和21世纪研究展望[J].植物病理学报, 1998, 28 (4) :289-298.
[3]	颜龙安, 张俊才, 朱成, 等.水稻显性雄性不育基因鉴定初报[J].作物学报, 1989, 15 (2) :174-181.
[4]	邓晓建, 周开达.低温敏显性核不育水稻“8987”的育性转换与遗传研究[J].四川农业大学学报, 1994, 12 (3) :376-382.
[5]	舒庆尧, 吴殿星, 夏英武, 等.60 Coγ射线辐照诱发创造水稻显性雄性核不育系[J].核农学报, 2000, 14 (5) :274-278.
[6]	朱旭东, RUTTER J N.显性雄性核不育突变体水稻的遗传鉴定[J].核农学报, 2000, 14 (5) :279-283.
[7]	黄显波, 田志宏, 邓则勤, 等.水稻三明显性核不育基因的初步鉴定[J].作物学报, 2008, 34 (10) :1865-1868.
[8]	ZHENG K L, HUANG N, J BENNETT, et al.PCR-based marker-assisted selection in rice breeding[C]//International Rice Research Institute.IRRI Discussion Paper Series, 1995:1-14.
[9]	PANAUD O, CHEN X, MCCOUCH S R.Development of microsatellite markers and characterization of simple sequence length polymorphism (SSLP) in rice (Oryza sative L.) [J].Mol Gen Genet, 1996, 252:597-607.
[10]	中华人民共和国农业部.水稻品种鉴定DNA指纹方法[S].第1版.北京:中国农业出版社, 2007:1-7.
[11]	程本义, 施勇烽, 沈伟峰, 等.南方稻区国家水稻区域试验品种的微卫星标记分析[J].中国水稻科学, 2007, 21 (1) :7-12.
[12]	高忠丽.攀登小麦育种新高峰[J].山西农业科学, 1979, (1) :13-15.
[13]	刘秉华, 杨丽.矮败小麦的选育及利用前景[J].科学通报, 1991, (4) :306-308.
[14]	李晓春, 朱汉如, 朱列萍, 等.小麦雄性不育基因Tal与Rhtlo基因的连锁遗传[J].浙江农业大学学报, 1991, 17 (2) :165-168.
[15]	刘秉华.小麦核不育性与轮回选择育种[M].北京:科学出版社, 1994.
[16]	李树林, 周熙荣, 周志疆, 等.显性核不育油菜的遗传与利用[J].作物研究, 1990, 4 (3) :27-32.
[17]	陆光远, 张学昆, 李桂英, 等.应用群体改良法选育高产优质抗病油菜新品种[J].中国油料作物学报, 2012, 34 (6) :575-581.
[18]	龚慧明, 贺浩华, 刘宜柏, 等.萍乡显性核不育水稻临界温度值及其温度敏感期研究[J].中国水稻科学, 2000, 14 (1) :19-23.
[19]	林成豹, 唐江霞, 邓则勤, 等.以三明显性核不育水稻为载体转导水稻抗稻瘿蚊基因Gm6[J].福建农业学报, 2013, 28 (5) :438-442.