Construction and Characterization of DNA Vaccine for Major Outer Membrane Protein Gene of Aeromonas hydrophila

LI Pan; LI Su-yi; WU Wei-wei; LIN Tian-long; LIN Chen-tao; CHEN Xu

doi:10.19303/j.issn.1008-0384.2013.09.002

Volume 28 Issue 9

Sep. 2013

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LI Pan, LI Su-yi, WU Wei-wei, LIN Tian-long, LIN Chen-tao, CHEN Xu. Construction and Characterization of DNA Vaccine for Major Outer Membrane Protein Gene of Aeromonas hydrophila[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 844-848. doi: 10.19303/j.issn.1008-0384.2013.09.002

Citation:

LI Pan, LI Su-yi, WU Wei-wei, LIN Tian-long, LIN Chen-tao, CHEN Xu. Construction and Characterization of DNA Vaccine for Major Outer Membrane Protein Gene of Aeromonas hydrophila[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 844-848. doi: 10.19303/j.issn.1008-0384.2013.09.002

Citation:

LI Pan, LI Su-yi, WU Wei-wei, LIN Tian-long, LIN Chen-tao, CHEN Xu. Construction and Characterization of DNA Vaccine for Major Outer Membrane Protein Gene of Aeromonas hydrophila[J]. Fujian Journal of Agricultural Sciences, 2013, 28(9): 844-848. doi: 10.19303/j.issn.1008-0384.2013.09.002

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Construction and Characterization of DNA Vaccine for Major Outer Membrane Protein Gene of Aeromonas hydrophila

doi: 10.19303/j.issn.1008-0384.2013.09.002

1. Fujian Agriculture and Forestry University;
2. Institute of Biotechnology,Fujian Academy of Agricultural Sciences;
3. Fujian Normal University

Received Date: 2013-07-14
Publish Date: 2013-09-18

Abstract

Abstract

For constructing the DNA vaccine, apair of specific PCR primers were designed according to the nucleotide sequence of Aeromonas hydrophila major outer membrane protein gene published by our lab previously. The PCR products were inserted into the eukaryotic expression vector pcDNA3 to construct a DNA vaccine named pcDNA3-POMP.SD rats were immunized with purified prokaryotic-expressed protein GST-POMP to make antiserum, whose antibody titer, measured by ELISA, was over 1∶100 000.293cells were transfected with pcDNA3-POMP, 48hours after transfection, the cells were collected for total RNA extraction and RT-PCR, and the expression of exogenous momp were detected.Therefore, we have constructed a plasmid of DNA vaccine for Aeromonas hydrophila, vaccination and evaluation of this DNA vaccine will be studied in the future.
- Aeromonas hydrophila,
- DNA vaccine,
- outer membrane protein

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References(12)

References

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