Effects of Exogenous Ca²⁺ on the Intracellular Calcium Distribution and Antioxidant Enzymes Activities of Eriobotryajaponica Seedlings and Leaves

CaCl2solution of different concentrattion were sprayed on the leaves of potted Eriobotryajaponica seedlings, the effects were verified by the calcium pyroantimonate precipitation method.Then, the seedlings were artificially treated at-3℃for 1.5h, and the cold resistance-relating physiological indices of the leaf tissues were measured.The results showed that CaCl2solutions of 0.05-50mmol·L-1 could all managed to decrease the MDA contents and to increase the SOD, POD, CAT and APX activities.The SOD activity of the control group was significantly elevated by 26.70% by using 10mmol·L-1 CaCl2solution.Although the APX activity of the control was maximally increased by 6.392% via 25 mmol·L-1 CaCl2solution, the upgrowth was still not significant.Thus, spraying appropriate CaCl2solution besides water in orchards prior to cold-air outbreaks was conducive to augmenting the cold-resistance indices of Eriobotryajaponica seedlings, thereby minimizing the low-temperature damages.