Study on Fermentation of Agaricus bisporus Strain W192

CENG Zhi-heng; CHENG Yi; CENG Hui; DING Zhong-wen; DAI Jian-qing

doi:10.19303/j.issn.1008-0384.2013.08.008

Volume 28 Issue 8

Aug. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(8): 763-769

CENG Zhi-heng, CHENG Yi, CENG Hui, DING Zhong-wen, DAI Jian-qing. Study on Fermentation of Agaricus bisporus Strain W192[J]. Fujian Journal of Agricultural Sciences, 2013, 28(8): 763-769. doi: 10.19303/j.issn.1008-0384.2013.08.008

Citation:

CENG Zhi-heng, CHENG Yi, CENG Hui, DING Zhong-wen, DAI Jian-qing. Study on Fermentation of Agaricus bisporus Strain W192[J]. Fujian Journal of Agricultural Sciences, 2013, 28(8): 763-769. doi: 10.19303/j.issn.1008-0384.2013.08.008

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Study on Fermentation of Agaricus bisporus Strain W192

doi: 10.19303/j.issn.1008-0384.2013.08.008

1. Edible Fungi Institute of Fujian Academy of Agricultural Sciences;
2. Fujian Academy of Agricultural Sciences

Received Date: 2013-06-24
Publish Date: 2013-08-18

Abstract

Abstract

Culture medium and conditions for the fermentation of Agaricus bisporus strain W192 were optimized using the respond surface method.Determined by a single factor test, the optimal carbon source for the mycelial development was cornstarch;the nitrogen source, soymeal;and, the inorganic salts, K2HPO4and MgSO4·7H2O.The fermentation was best carried out in a liquid medium with an initial pH of 6.5in a flask held at 25℃and shaken constantly at 180r·min-1 for 7days.Using the Plackett-Burman design, the conditions for the A.bisporus mycelium biomass culture were evaluated.Cornstarch, soymeal and flask-shaking speed were found to be the most prominent factors that affected the fermentation.Through the respond surface analysis, a regression equation was obtained, and the fermentation conditions optimized as cornstarch at 43.67g·L-1, soymeal at 13.78g·L-1, K2HPO4at 3.0g·L-1, MgSO4·7H2O at 1.0g·L-1, incubation at 25℃, flask-shaking at 200r·min-1, medium initial pH at 6.5, and fermentation for 7days.The resultant A.bisporus mycelium biomass reached a concentration of 2.58g per 100mL, which was 2.35times higher than the orginal conditions.
- Agaricus bisporus,
- W192,
- fermentation,
- respond surface method,
- optimization

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References(10)

References

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