Optimization of Tissue Culture for Propagating Clustered Shoots of Phalaenopsis

ZHONG Hai-feng; ZHONG Huai-qin; HUANG Min-ling; WU Jian-she; YE Xiu-xian

doi:10.19303/j.issn.1008-0384.2013.07.011

Volume 28 Issue 7

Jul. 2013

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(7): 675-679

ZHONG Hai-feng, ZHONG Huai-qin, HUANG Min-ling, WU Jian-she, YE Xiu-xian. Optimization of Tissue Culture for Propagating Clustered Shoots of Phalaenopsis[J]. Fujian Journal of Agricultural Sciences, 2013, 28(7): 675-679. doi: 10.19303/j.issn.1008-0384.2013.07.011

Citation:

ZHONG Hai-feng, ZHONG Huai-qin, HUANG Min-ling, WU Jian-she, YE Xiu-xian. Optimization of Tissue Culture for Propagating Clustered Shoots of Phalaenopsis[J]. Fujian Journal of Agricultural Sciences, 2013, 28(7): 675-679. doi: 10.19303/j.issn.1008-0384.2013.07.011

Citation:

PDF( 1434 KB)

Optimization of Tissue Culture for Propagating Clustered Shoots of Phalaenopsis

doi: 10.19303/j.issn.1008-0384.2013.07.011

1. Institute of Crop sciences,Fujian Academy of Agriculture Science;
2. Flowers Research Center,Fujian Academy of Agricultural Science;
3. Fujian Engineering Research Certer for Characteristic Floriculture

Received Date: 2013-02-17
Publish Date: 2013-07-18

Abstract

Abstract

Adventitious shoots induced from the peduncle auxiliary buds of Phalaenopsis‘Lauluns' were used for the study.The physical segmentation, placement mode and concentration of inorganic salt and organic additives all showed significant effects on the proliferation of Phalaenopsis with clustered shoots.Among the treatments, the greatest average number of inductive buds occurred when a bud was excised at the growth point and then grafted horizontally (PSP-3) .However, the bud's death rate was as high as 23.3%.In contrast, when the double buds were used, and followed by the horizontal grafting (PSP-6) , the budding was high, but the death rate was low.The inorganic salt concentration between 1/3MS to 1/2MS was found to be suitable for the propagating with strong seedling growth.Addition of coconut juice at 15% (v/v) level was desirable for the Phalaenopsis cluster buds proliferation, while 150g·L-1 potato juice or banana puree could be applied as low cost organic additives in the culture medium.
- Phalaenopsis,
- clustered shoots,
- proliferation,
- inorganic salt,
- organic additive,
- apical dominance

FullText(HTML)

References(19)

References

[1]	李军, 柴向华, 曾宝珰, 等.蝴蝶兰组培工厂化生产技术[J].园艺学报, 2004, (3) :413-414.
[2]	潘学峰, 王安石, 李海珠.利用丛芽途径快速繁殖蝴蝶兰的研究[J].海南大学学报:自然科学版, 2005, 23 (1) :47-60.
[3]	王云惠, 陈雄庭.蝴蝶兰组织培养与基因工程育种研究进展[J].安徽农业科学, 2007, 35 (16) :4827-4830 , 4868.
[4]	刘亮, 易自力, 蒋建雄, 等.蝴蝶兰丛生芽、原球茎途径的组织培养研究[J].亚热带植物科学, 2008, 37 (3) :43-45.
[5]	陈之林, 叶秀粦, 梁承邺.蝴蝶兰花葶的离体培养[J].园艺学报, 2003, 30 (2) :242-244.
[6]	周俊辉, 叶超宏, 陈旭高.蝴蝶兰原球茎增殖培养的研究[J].仲恺农业技术学院学报, 2002, 15 (3) :13-17.
[7]	王静, 娄玉霞, 郝再彬, 等.大量元素、有机添加物、激素对蝴蝶兰原球茎增殖的影响[J].上海农业科技, 2004, (3) :21-23.
[8]	邹金环, 赵大勇, 刘艳梅, 等.蝴蝶兰组织培养快繁技术研究[J].北方园艺, 2005, (6) :86-87.
[9]	曹孜义, 刘国民.实用植物组织培养技术教程[M].甘肃:甘肃科学技术出版社, 2002:179-181.
[10]	ORLIKOWSKA T, SABALA I, KUCHARSKA D.The effect of leaf and shoot tip removal and explant orientation on axillary shoot proliferation of Codiaeum vanegatum Blume var.pictum Muell[J].Arg cv Excellent Scientia Hort, 2000, 85:103-111.
[11]	吴宣萱, 陈福旗.蝴蝶兰与朵丽蝶兰花梗芽之诱导与刻伤处理对芽体增殖之影响[J].台湾园艺, 2008, 54 (1) :67-74.
[12]	VARSHA S, VINAY K, MAHADEO G S.Indirect organogenesis and plant regeneration in Helicteres isora L., an important medicinal plant[J].In Vitro Cell Dev BiolPlant, 2008, 44 (3) :186-193.
[13]	何松林, 孔德政, 杨秋生, 等.碳源和有机添加物对文心兰原球茎增殖的影响[J].河南农业大学学报, 2003, 37 (2) :154-157.
[14]	AL-KHATEEB A A.Regulation of in vitro bud formation of date palm (Phoenix dactylifera L.) cv.Khanezi by different carbon sources[J].Bio resource Technol, 2008, 99 (14) :6550-6555.
[15]	GNASKARANn P, XAVIER R, UMA RANI S, et al.A study on the use of organic additives on the protocorm-like bodies (PLBs) growth of Phalaenopsis violacea orchid[J].J Phytol, 2010, 2 (1) :29-33.
[16]	MURDAD R, LATIP M A, AZIZ Z A, et al.Effects of carbon source and potato homogenate on in vitro growth and development of Sabah′s endangered orchid:Phalaenopsis gigantea[J].Asia-Pac J Mol Biol, 2010, 18 (1) :199-202.
[17]	刘明志, 朱京育.培养基、BA和复合添加物对大花蕙兰增殖和分化的影响[J].暨南大学学报:自然科学与医学版, 2000, 21 (3) :100-105.
[18]	刘晓燕, 向青云, 刘玲玲, 等.基本培养基及附加物对蝴蝶兰原球茎增殖效果的影响[J].种子, 2005, 24 (6) :18-21.
[19]	何松林, 蒋要卫, 任凝辉, 等.培养基、添加物及碳源对蝴蝶兰试管苗生长的影响[J].河南农业大学学报, 2006, 40 (2) :142-145.