Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus

PAN Yi-zhe; WAN Chun-he; HUANG Yu; CHENG Long-fei; FU Guang-hua; SHI Shao-hua; CHEN Hong-mei

doi:10.19303/j.issn.1008-0384.2013.07.005

Volume 28 Issue 7

Jul. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(7): 644-647

PAN Yi-zhe, WAN Chun-he, HUANG Yu, CHENG Long-fei, FU Guang-hua, SHI Shao-hua, CHEN Hong-mei. Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(7): 644-647. doi: 10.19303/j.issn.1008-0384.2013.07.005

Citation:

PAN Yi-zhe, WAN Chun-he, HUANG Yu, CHENG Long-fei, FU Guang-hua, SHI Shao-hua, CHEN Hong-mei. Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(7): 644-647. doi: 10.19303/j.issn.1008-0384.2013.07.005

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Prokaryotic Expression of the NS1 Gene for Avian Tembusu Virus

doi: 10.19303/j.issn.1008-0384.2013.07.005

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Science;
2. College of Animal Science and Technology,Jiangxi Agricultural University

Received Date: 2013-02-03
Publish Date: 2013-07-18

Abstract

Abstract

A pair of primers were designed and synthesized according to the nanostructure protein (NS1) gene of avian Tenbusu virus WR strain available in GenBank.The NS1gene was amplified by RT-PCR and cloned into the prokaryotic expression vector pGEX-4T-1, then induced into E.coli with IPTG.The SDS-PAGE analysis of expressed protein demonstrated that the recombinant NS1protein of 62kD had got a high expression, which laid a good foundation for developing in molecular diagnosis and the function of the NS1gene for avian Tenbusu virus.
- avian Tenbusu virus,
- NS1gene,
- prokaryotic expression

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References(20)

References

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