Extraction and Deproteinization Technology for Crude Polysaccharides in Radix Pseudostellariae

WU Bin; XIE Yong; LIN Xiu-jie; LIU Zeng-bin; FAN Hai-ping

doi:10.19303/j.issn.1008-0384.2013.06.017

Volume 28 Issue 6

Jun. 2013

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WU Bin, XIE Yong, LIN Xiu-jie, LIU Zeng-bin, FAN Hai-ping. Extraction and Deproteinization Technology for Crude Polysaccharides in Radix Pseudostellariae[J]. Fujian Journal of Agricultural Sciences, 2013, 28(6): 589-592. doi: 10.19303/j.issn.1008-0384.2013.06.017

Citation:

WU Bin, XIE Yong, LIN Xiu-jie, LIU Zeng-bin, FAN Hai-ping. Extraction and Deproteinization Technology for Crude Polysaccharides in Radix Pseudostellariae[J]. Fujian Journal of Agricultural Sciences, 2013, 28(6): 589-592. doi: 10.19303/j.issn.1008-0384.2013.06.017

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Extraction and Deproteinization Technology for Crude Polysaccharides in Radix Pseudostellariae

doi: 10.19303/j.issn.1008-0384.2013.06.017

1. Freshwater Fisheries Research Institute of Fujian,Fuzhou,Fujian 350002,China;
2. Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350122,China

Received Date: 2013-05-06
Publish Date: 2013-06-18

Abstract

Abstract

Polysaccharide is one of the active ingredients extracted fromradix pseudostellariae.In this study,the solid-liquid ratio,temperature,extracting duration and times in crude polysaccharide extraction of radix pseudostellariae were investigated by hot water bath,using both the single-factor test and L9(34)orthogonal test.The results showed that the optimum parameters for extraction were with the ratio of solid to liquid 1∶20,extracting temperature 100℃,reflux extraction time 90min and extraction for 3times.The deproteinization effects were compared among three methods,the Sevag reagent,trichloroacetic acid,and trichloroacetic acid-butyl alcohol,and the results presented that trichloroacetic acid-butyl alcohol was the best for deproteinization,with 81.2% crued polysacchsrides and 9.93% protein,indicating that the deproteinization rate was 60.14%.
- radix pseudostellariae,
- crued polysacchsrides,
- deproteinization

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References(18)

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