Studying on In Vitro Culture of Fujian Wild Grapes

LAI Cheng-chun; FAN Li-hua; HUANG Xian-gui; XIE Hong-gen; YU Ya-bai

doi:10.19303/j.issn.1008-0384.2013.06.007

Volume 28 Issue 6

Jun. 2013

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(6): 541-544

LAI Cheng-chun, FAN Li-hua, HUANG Xian-gui, XIE Hong-gen, YU Ya-bai. Studying on In Vitro Culture of Fujian Wild Grapes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(6): 541-544. doi: 10.19303/j.issn.1008-0384.2013.06.007

Citation:

LAI Cheng-chun, FAN Li-hua, HUANG Xian-gui, XIE Hong-gen, YU Ya-bai. Studying on In Vitro Culture of Fujian Wild Grapes[J]. Fujian Journal of Agricultural Sciences, 2013, 28(6): 541-544. doi: 10.19303/j.issn.1008-0384.2013.06.007

Citation:

PDF( 1176 KB)

Studying on In Vitro Culture of Fujian Wild Grapes

doi: 10.19303/j.issn.1008-0384.2013.06.007

Institute of Agricultural Engineering and Technology,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2013-03-14
Publish Date: 2013-06-18

Abstract

Abstract

In vitro culture technique of Fujian wild grapes was studied by using the stem-segment with node as explants.The results showed that their axillary buds could be induced in MS medium supplemented with 2.0mg.L-1 BA and 0.2mg.L-1 NAA,in which the induction rate was 72.2%.Considering multiplication coefficient and plantlet quality comprehensively,the medium MS or 1/2MS added with 2.0mg.L-1 BA and 0.2mg.L-1 NAA was the best for the proliferation of fascicular gemma with the propagation coefficient over than 3.0.For in vitro germplasm conservation of Fujian wild grape plantlets,the suitable medium was MS with 0.1mg.L-1 NAA and the plastochrone of subculture were about 5to 6mouths.Consequently,the growth ability of plantlets could be maintained vigorously.
- wild grape,
- In vitro culture,
- stem-segment,
- explant,
- germplasm conservation

FullText(HTML)

References(12)

References

[1]	孔庆山.中国葡萄志[M].北京:中国农业科学技术出版社,2004:16-27.
[2]	刘崇怀,孔庆山.我国野生葡萄资源的保存现状及建议[J].中外葡萄与葡萄酒,2008,(1):42-46.
[3]	李朝銮,曹亚玲,何永华.中国葡萄属(Vitis L.)分类研究[J].应用与环境生物学报,1996,2(3):234-253.
[4]	阮仕立,李记明.野生葡萄种质资源的抗性及其利用研究进展[J].中外葡萄与葡萄酒,2002,(4):30-33.
[5]	邹瑜,莫磊兴,林贵美,等.野生毛葡萄茎段离体培养和生产应用研究[J].广西农业科学,1998,(4):196-199.
[6]	陈祖玉,石雪晖,杨梦玲,等.野生葡萄离体培养研究[J].中外葡萄与葡萄酒,2002,(4):22-24.
[7]	RAO N K.Plant genetic resources:Advancing conservationand use through biotechnology[J].African Journal ofBiotechnology,2004,3(2):136-145.
[8]	ENGELMANN F.In vitro conservation of tropical plantgermplasm-a review[J].Euphytica,1991,57:227-243.
[9]	金佩芳,蒋爱丽,李世诚.我国野生葡萄试管苗的建立研究[J].葡萄栽培与酿酒,1995,72(1):31-34.
[10]	张剑侠,王跃进,李沛玲,等.中国野生葡萄的离体培养与快速繁殖[J].园艺学报,2004,31(1):90-93.
[11]	刘伟,潘学军,张文娥,等.贵州野生毛葡萄茎段离体快繁[J].山地农业生物学报,2009,28(4):60-362.
[12]	SAJID G N,ILYAX M K,NWAR R.Effect of diversehormonal regimes on in vitro growth of grape germplasm[J].Pakistan Journal of Botany,2006,38(2):385-391.