Optimizing SRAP Reaction System for Abelmoschus esculentus Linn.

LI Yong-ping; XUE Zhu-zheng; LIN Hun; WEN Qing-fang

doi:10.19303/j.issn.1008-0384.2013.05.012

Volume 28 Issue 5

May 2013

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LI Yong-ping, XUE Zhu-zheng, LIN Hun, WEN Qing-fang. Optimizing SRAP Reaction System for Abelmoschus esculentus Linn.[J]. Fujian Journal of Agricultural Sciences, 2013, 28(5): 468-471. doi: 10.19303/j.issn.1008-0384.2013.05.012

Citation:

LI Yong-ping, XUE Zhu-zheng, LIN Hun, WEN Qing-fang. Optimizing SRAP Reaction System for Abelmoschus esculentus Linn.[J]. Fujian Journal of Agricultural Sciences, 2013, 28(5): 468-471. doi: 10.19303/j.issn.1008-0384.2013.05.012

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Optimizing SRAP Reaction System for Abelmoschus esculentus Linn.

doi: 10.19303/j.issn.1008-0384.2013.05.012

1. Institute of Crop Sciences,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;
2. Vegetable Centre,Fujian Academy of the Agricultural Sciences,Fuzhou,Fujian 350013,China;
3. Engineering Research Center for Vegetable,Fujian Academy of the Agricultural Sciences,Fuzhou,Fujian 350013,China

Received Date: 2012-11-12
Publish Date: 2013-05-18

Abstract

Abstract

Through optimization of the concentrations of Taq DNA polymerase,dNTP,primers and DNA template,SRAP-PCR of Abelmoschus esculentus Linn.was established.The results indicated that,in a 20μL reaction mixture,the optimal concentration of genomic DNA template was 75ng;that of the primers,0.4mmol·L-1;that of dNTP,0.75mmol·L-1;that of Taq DNA,polymerase,1.5U;and that of MgCl2,2.0mmol·L-1.
- Abelmoschus esculentus Linn.,
- genetic relationship,
- SRAP

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References(15)

References

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