Factors Affecting Maturation of Somatic Embryos of Slash Pine

WU Li-jun; WENG Qiu-yuan; CHEN Da

doi:10.19303/j.issn.1008-0384.2013.04.014

Volume 28 Issue 4

Apr. 2013

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WU Li-jun, WENG Qiu-yuan, CHEN Da. Factors Affecting Maturation of Somatic Embryos of Slash Pine[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 372-376. doi: 10.19303/j.issn.1008-0384.2013.04.014

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WU Li-jun, WENG Qiu-yuan, CHEN Da. Factors Affecting Maturation of Somatic Embryos of Slash Pine[J]. Fujian Journal of Agricultural Sciences, 2013, 28(4): 372-376. doi: 10.19303/j.issn.1008-0384.2013.04.014

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Factors Affecting Maturation of Somatic Embryos of Slash Pine

doi: 10.19303/j.issn.1008-0384.2013.04.014

Fujian Academy of Forestry,Fuzhou,Fujian 350012,China

Received Date: 2013-03-14
Publish Date: 2013-04-18

Abstract

Abstract

Using 4embryogenesis cell lines(ECLs),which were the embryogenic suspensor mass(ESM)of the slash pine,critical factors affecting the maturation of somatic embryos(SEs)were identified and studied.The results showed that different ECLs and carbon sources in the medium were the key factors affecting the maturation of the SEs.The presence of PEG of different molecular weights in different concentrations with ABA and AC in the medium affected SEs both qualitatively and quantitatively.The most desirable medium for the embryo maturation was found to consist of LP+ABA70mg.L-1+PEG 8000 150g.L-1+maltose 60g.L-1+AC 0~2.0g.L-1+ agar 3.0g.L-1.On which,approximately 19.8mature cotyledonary embryos were harvested from 1cm diameter of ESM.
- Pinus elliottii,
- maturation of somatic embryos,
- factors

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References(17)

References

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