Construction and Verification of Trans-linked Double T-DNA Plant Expression Vector

HU Tai-jiao; FENG Qing-ling; PAN Da-ren; WANG Feng

doi:10.19303/j.issn.1008-0384.2013.03.001

Volume 28 Issue 3

Mar. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(3): 195-200

HU Tai-jiao, FENG Qing-ling, PAN Da-ren, WANG Feng. Construction and Verification of Trans-linked Double T-DNA Plant Expression Vector[J]. Fujian Journal of Agricultural Sciences, 2013, 28(3): 195-200. doi: 10.19303/j.issn.1008-0384.2013.03.001

Citation:

HU Tai-jiao, FENG Qing-ling, PAN Da-ren, WANG Feng. Construction and Verification of Trans-linked Double T-DNA Plant Expression Vector[J]. Fujian Journal of Agricultural Sciences, 2013, 28(3): 195-200. doi: 10.19303/j.issn.1008-0384.2013.03.001

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Construction and Verification of Trans-linked Double T-DNA Plant Expression Vector

doi: 10.19303/j.issn.1008-0384.2013.03.001

1. The College of Life Sciences,Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China;
2. Biotechnology Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2013-02-05
Publish Date: 2013-03-18

Abstract

Abstract

We constructed two trans-linked double T-DNA plant expression vectors by modification of the structure of the double T-DNA,p1300-DMAR-LF-GCMSAGS and p1300-DMAR-LF-GLCMSAGS.They were verified by transgenic rice plants.The results indicated that the selectable marker-free transgenic plants could be obtained.The frequencies of unlinked co-transformants transformed by the trans-linked double T-DNA plant expression vectors were increased from 47.37% and 45.83% to 55.81% and 51.28% compared to those transformed by the cis-linked double T-DNA plant expression vectors,indicated that the efficiency on the selectable marker-free transgenic plant was improved.
- double T-DNA,
- trans-linked,
- plant expression

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References(9)

References

[1]	MILLER M,TAGLIANI L,WANG N,et al.High efficiencytransgene segregation in co-transformed maize plants using anAgrobacterium tumefaciens 2 T-DNA binary system[J].Transgenic Res,2002,11(4):381-396.
[2]	KOMARI T,HIEI Y,SAITO Y,et al.Vectors carrying twoseparate T-DNAs for co-transformation of higher plantsmediated by Agrobacterium tumefaciens and segregation oftransformants free from selection markers[J].Plant J,1996,10(1):165-174.
[3]	ZHOU H Y,CHEN S B,LI X G,et al.Generating marker-free transgenic tobacco plants by Agrobacterium-mediatedtransformation with double T-DNA binary vector[J].Bulletinof Botany,2003,45(9):1103-1108.
[4]	BREITLER J C,MEYNARD D,BOXTEL J V,et al.A noveltwo T-DNA binary vector allows efficient generation of marker-free transgenic plants in three elite cultivars of rice(Oryzasativa L.)[J].Transgenic Res,2004,13:271-287.
[5]	MATTHEWS P R,WANG M B,WATERHOUSE P M,et al.Marker gene elimination from transgenic barley,using co-transformation with adjacent“twin T-DNAs”on a standardAgrobacterium transformation vector[J].Mol Breeding,2001,7:195-202.
[6]	朱祯,李旭刚.一种利用双T-DNA载体培育无选择标记的转基因水稻的方法[P].中国专利:2005-08-10;02107429.
[7]	CHU C C.The N6 medium and its applications to antherculture of cereal crops[M].Proceedings of Symposium in PlantTissue Culture,北京:科学出版社,1978:43-50.
[8]	BAI X Q,WANG Q Y,CHU C C.Excision of a selectivemarker in transgenic rice using a novel Cre/loxP systemcontrolled by a floral specific promoter[J].Transgenic Res,2008,17:1035-1043.
[9]	MURRAY M G,THOMPSON W F.Rapid isolation of highmolecular weight plant DNA[J].Nucleic Acids Res,1988,8:4321-4326.