Cloning and Construction of Over-expression Vector of OsAPX Gene

XU Hui-bin; LIAN Ling; WEI Yi-dong; ZHU Yong-sheng; XIE Hua-an; WANG Zong-hua; ZHANG Jian-fu

doi:10.19303/j.issn.1008-0384.2013.02.003

Volume 28 Issue 2

Feb. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(2): 101-106

XU Hui-bin, LIAN Ling, WEI Yi-dong, ZHU Yong-sheng, XIE Hua-an, WANG Zong-hua, ZHANG Jian-fu. Cloning and Construction of Over-expression Vector of OsAPX Gene[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 101-106. doi: 10.19303/j.issn.1008-0384.2013.02.003

Citation:

XU Hui-bin, LIAN Ling, WEI Yi-dong, ZHU Yong-sheng, XIE Hua-an, WANG Zong-hua, ZHANG Jian-fu. Cloning and Construction of Over-expression Vector of OsAPX Gene[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 101-106. doi: 10.19303/j.issn.1008-0384.2013.02.003

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Cloning and Construction of Over-expression Vector of OsAPX Gene

doi: 10.19303/j.issn.1008-0384.2013.02.003

XU Hui-bin^1,2,3,4,5,
LIAN Ling^2,3,4,5,
WEI Yi-dong^1,2,3,4,5,
ZHU Yong-sheng^2,3,4,5,
XIE Hua-an^2,3,4,5,
WANG Zong-hua¹,
ZHANG Jian-fu^2,3,4,5

1. College of Life Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;
2. Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China,Ministry of Agriculture,P.R China/Fuzhou branch,National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/ Fujian Key Laboratory of Rice Molecular Breeding/ Rice Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian.350003,China;
3. Incubator of National key Laboratory of Fujian Germplasm Innovation and Molecular Breeding Between Fujian and Ministry of Sciences & Technology,P.R.China,Fuzhou,Fujian 350003,China;
4. South China Bases of National Key Laboratory of Hybrid Rice for China,Fuzhou,Fujian 350003,China;
5. National Rice Engineering Laboratory of China,Fuzhou,Fujian 350003,China

Received Date: 2012-12-12
Publish Date: 2013-02-18

Abstract

Abstract

A full length cDNA of ascorbate peroxidase(APX) gene was cloned from Oryza sativa L.Japonica Nipponbare with RT-PCR.It was 768 bp including the CDS,and was connected to the plant expression binary vector,pBI121,to construct an over-expression vector of APX gene.The pBI121-APX vector was transformed into indica rice,Duoxi No.1 and Hang No.1,mediating by Agrobacterium tumefaciens EHA105.With this,transgenic plants could be obtained for further study on the function of APX gene in rice storability.
- clone,
- APX,
- over-expression vector,
- genetic transformation

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References(25)

References

[1]	BEWLEY J D.Membrane changes in seeds as related togermination and the perturbation resulting from deteriorationin storage[J].Crop Science Society of America,1984,11:27-45.
[2]	LEPRINCE O,BUITINK J,HOEKSTRA FA.Radicles andcotyledons of recalcitrant seeds of Castanea sativa Mill.exhibitcontrasting responses of respiration to drying in relation todesiccation sensitivity[J].Journal Experimental Botany,1999,338:1515-1524.
[3]	FINKEL T,HOLBROOK N J.Oxidants,oxidative stress andthe biology of ageing[J].Nature,2000,408:239-247.
[4]	KANNAN K,JAIN S K.Oxidative stress and apoptosis[J].Pathophysiology,2000,7:153-163.
[5]	HALLIWELL B,GUTTERIDGE J M C.Free radicals inbiology and mMedicine[N].Oxford University Press,2002(3).
[6]	HOEKSTRA F A,WOLKERS W F,BUITINK J,et al.Membrane stabilization in the dried state[J].ComparativeBiochemistry and Physiology,1997,117A:335-341.
[7]	HENDRY G A,FINCH-SAVAGE W E,THORPE P C,et al.Free radical processes and loss of seed viability duringdesiccation in the recalcitrant species Quercus robur L.[J].New Phytologist,1992,122:273-279.
[8]	LEPRINCE O,HENDRY G A F,MCKERSIE B D.Themechanism of desiccation tolerance in developing seeds[J].Seed Science Research,1993,3:231-246.
[9]	VERTUCCI C W,FARRANT J M.Acquisition and loss ofdesiccation tolerance[N].Seed development and germination,1995,237-271.
[10]	SMITH M T,BERJAK P.Deteriorative changes associatedwith the loss of viability of stored desiccation-Tolerant anddesiccation-sensitive seeds[N].1995,701-746.
[11]	MCDONALD M B.Seed deterioration:physiology,repair andassessment[J].Seed Science and Technology,1999,27:177-237.
[12]	BEWLEY J D,BLACK M.Seeds:physiology of developmentand germination[N].Plenum Press,1994.
[13]	BEWLEY J D.Membrane changes in seeds as related togermination and the perturbation resulting from deteriorationin storage[J].Crop Science Society of America,1986,11:27-45.
[14]	PARISH D J,LEOPOLD A C,HANNA M A.Turgorchanges with accelerated ageing of soybeans[J].CropScience,1982,22:666-669.
[15]	ROBERTS E H.Cytological,genetical and metabolic changesassociated with loss of viability[J].Viability of seeds,1972:253-306.
[16]	MAGUIRE J D.Seed quality and germination[J].Thephysiology and biochemistry of seed dormancy andgermination,1977:219-235.
[17]	BAILLY C.Active oxygen species and antioxidant in seedbiology[J].Seed Science Research,2004,14:93-107.
[18]	WILSON J O,MCDONALD J B.The lipid peroxidation modelof seed ageing[J].Seed Science and Technology,1986,14:269-300.
[19]	ALLEN R D.Dissection of oxidative stress tolerance usingtransgenic plants[J].Plant Physiology,1995,107:1049-1054.
[20]	HALLIWELL B,GUTTERIDGE J M C.Free radicals inbiology and Medicine[N],Oxford University Press,2002(3).
[21]	KWON S Y,JEONG Y J,LEE H S,et al.Enhancedtolerances of transgenic tobacco plants expressing bothsuperoxide dismutase and ascorbate peroxidase in chloroplastsagainst methyl viologen-mediated oxidative stress[J].Plant,Cell and Environment,2002,25:873-882.
[22]	YOUNG P L,KWANG-HYUN B,HAENG-SOON L,et al.Tobacco seeds simultaneously over-expressing Cu/Zn-superoxide dismutase and ascorbate peroxidase displayenhanced seed longevity and germination rates under stressconditions[J].Journal of Experimental Botany,2010,61(9):2499-2506.
[23]	BAILLY C.Active oxygen species and antioxidant in seedbiology[J].Seed Science Research,2004,14:93-107.
[24]	WILSON J D,MCDONALD J B.The lipid peroxidation modelof seed ageing[J].Seed Science and Technology,1986,14:269-300.
[25]	BAILLY C,AUDIGIER C,LADONNE F,et al.Changes inoligosaccharide content and antioxidant enzyme activities indeveloping bean seeds as related to acquisition of dryingtolerance and seed quality[J].Journal of ExperimentalBotany,2001,52:701-708.