Chloroplast Targeting Signal of a Rice Rubisco Activase Gene Enhances Transgene Expression

LI Xiang; LIN Zhi-min; CHEN Zai-jie; WANG Cheng-hu; LIU Xia; WANG Feng

doi:10.19303/j.issn.1008-0384.2013.02.002

Volume 28 Issue 2

Feb. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(2): 95-100

LI Xiang, LIN Zhi-min, CHEN Zai-jie, WANG Cheng-hu, LIU Xia, WANG Feng. Chloroplast Targeting Signal of a Rice Rubisco Activase Gene Enhances Transgene Expression[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 95-100. doi: 10.19303/j.issn.1008-0384.2013.02.002

Citation:

LI Xiang, LIN Zhi-min, CHEN Zai-jie, WANG Cheng-hu, LIU Xia, WANG Feng. Chloroplast Targeting Signal of a Rice Rubisco Activase Gene Enhances Transgene Expression[J]. Fujian Journal of Agricultural Sciences, 2013, 28(2): 95-100. doi: 10.19303/j.issn.1008-0384.2013.02.002

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Chloroplast Targeting Signal of a Rice Rubisco Activase Gene Enhances Transgene Expression

doi: 10.19303/j.issn.1008-0384.2013.02.002

1. School of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350000,China;
2. Biotechnology Institute,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350003,China

Received Date: 2012-11-12
Publish Date: 2013-02-18

Abstract

Abstract

N-terminal sequence of the protein encoded by rice RCA gene was predicted to be a chloroplast transit peptide.The 144bp fragment of this gene was fused with GFP to construct a transient expression vector pGD-RCATP-GFP for genetic transformation.Transient transformation of tobacco was produced by the Agrobacterium-mediated infiltration method.Fluorescence microscopy analyses demonstrated that,in cells expressing the RCATP:GFP fusion protein,the fluorescence was specifically targeted to chloroplast.Then this fragment was fused with GUS to construct a vector p1300-RCATP-AGS for genetic transformation.To analyze GUS activity for the leaves of the positive plants,we discovered that the RCA TP sequence increased GUS protein expression levels about 3 fold,when compared to without RCA TP sequence.
- RCA(Rubisco activase),
- chloroplast transit peptide,
- GUS activity analysis

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References(15)

References

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