Establishment of an Indirect ELISA for Detecting Egg Yolk Antibody Against Chicken Flavivirus

SHI Shao-hua; FU Guang-hua; CHENG Long-fei; WANG Jian; CHEN Hong-mei; WAN Chun-he; HU Si-ke; SONG Xiu-mei; HUANG Yu

doi:10.19303/j.issn.1008-0384.2013.01.006

Volume 28 Issue 1

Jan. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(1): 18-21

SHI Shao-hua, FU Guang-hua, CHENG Long-fei, WANG Jian, CHEN Hong-mei, WAN Chun-he, HU Si-ke, SONG Xiu-mei, HUANG Yu. Establishment of an Indirect ELISA for Detecting Egg Yolk Antibody Against Chicken Flavivirus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(1): 18-21. doi: 10.19303/j.issn.1008-0384.2013.01.006

Citation:

SHI Shao-hua, FU Guang-hua, CHENG Long-fei, WANG Jian, CHEN Hong-mei, WAN Chun-he, HU Si-ke, SONG Xiu-mei, HUANG Yu. Establishment of an Indirect ELISA for Detecting Egg Yolk Antibody Against Chicken Flavivirus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(1): 18-21. doi: 10.19303/j.issn.1008-0384.2013.01.006

Citation:

SHI Shao-hua, FU Guang-hua, CHENG Long-fei, WANG Jian, CHEN Hong-mei, WAN Chun-he, HU Si-ke, SONG Xiu-mei, HUANG Yu. Establishment of an Indirect ELISA for Detecting Egg Yolk Antibody Against Chicken Flavivirus[J]. Fujian Journal of Agricultural Sciences, 2013, 28(1): 18-21. doi: 10.19303/j.issn.1008-0384.2013.01.006

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Establishment of an Indirect ELISA for Detecting Egg Yolk Antibody Against Chicken Flavivirus

doi: 10.19303/j.issn.1008-0384.2013.01.006

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350013,China;
2. Fujian Animal Diseases Control Technology Development Center,Fuzhou, Fujian 350013,China;
3. Tianjin Zhongsheng Tiaozhan Biological Engineering.Inc.,Tianjin 300380,China

Received Date: 2012-11-09
Publish Date: 2013-01-18

Abstract

Abstract

A novel Flavivirus infection,which mainly caused egg-dropping in ducks and chickens,has spread throughout China since 2010.To carry out the serological surveillance in chickens with minimum stress,an indirect enzyme-linked immuosorbent assay(ELISA) for detecting egg yolk antibody against Flavivirus in layer chickens has been developed by coating plate with chicken-origin Flavivirus FQ-C1 isolate.The optimal working concentration of antigen was 5.7 ng ·μL-1,the working concentration of egg yolk antibody was 1∶160 dilution and that of HRP-labeled goat anti-chicken IgG was 1∶3 200.After a color reaction of 10 minutes with Tetramethylbenzidine(TMB) liquid substrate,a threshold of positive/negative(P/N) ≥ 2.1 was determined as positive standard for the ELISA.The results indicated that the ELISA established by this research provided a high sensitivity and specificity test,which was suitable for the detection of antibodies against Flavivirus in immune laying hens.
- chicken-origin Flavivirus,
- indirect ELISA,
- egg yolk antibody

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References(12)

References

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