Genetic Characteristics of ORF1 Gene of Porcine Torque Teno Virus Genogroup 2 in Fujian

XIU Jin-sheng; CHEN Ru-jing; WU Xue-min; WANG Bin; LI Tao; LIN Qun-qun

doi:10.19303/j.issn.1008-0384.2013.01.005

Volume 28 Issue 1

Jan. 2013

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Article Navigation > Fujian Journal of Agricultural Sciences > 2013 > 28(1): 14-17

XIU Jin-sheng, CHEN Ru-jing, WU Xue-min, WANG Bin, LI Tao, LIN Qun-qun. Genetic Characteristics of ORF1 Gene of Porcine Torque Teno Virus Genogroup 2 in Fujian[J]. Fujian Journal of Agricultural Sciences, 2013, 28(1): 14-17. doi: 10.19303/j.issn.1008-0384.2013.01.005

Citation:

XIU Jin-sheng, CHEN Ru-jing, WU Xue-min, WANG Bin, LI Tao, LIN Qun-qun. Genetic Characteristics of ORF1 Gene of Porcine Torque Teno Virus Genogroup 2 in Fujian[J]. Fujian Journal of Agricultural Sciences, 2013, 28(1): 14-17. doi: 10.19303/j.issn.1008-0384.2013.01.005

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Genetic Characteristics of ORF1 Gene of Porcine Torque Teno Virus Genogroup 2 in Fujian

doi: 10.19303/j.issn.1008-0384.2013.01.005

1. College of Animal Science,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China;
2. Institute of Animal Husbandry and Veterinary Medicine/Fujian Animal Diseases Control Technology Development Center,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China

Received Date: 2012-10-14
Publish Date: 2013-01-18

Abstract

Abstract

The ORF1 Gene of porcine torque teno virus genogroup 2 was amplified by PCR method from the piglets sera genome association with post-weaning multisystem wasting syndrome from Fujian,the target product was cloned and sequenced.The results demonstrated that the porcine torque teno virus genogroup 2 ORF1 gene was 1875 bp in length,coding an open reading frame(ORF) with 624 amino acids.The nucleotide sequence and the amino acid sequence deduced from the gene were analyzed by the bioinformatics software.Compared with the porcine TTV2 ORF1 genes download from GenBank,the sequenced gene shared the highest homogeneity with FJ/China/2010/TTV2/2(GenBank accession number JF937656) strain at 98.8%,but shared lower homogeneity with SC strain(GenBank accession number HQ204188) strain only at 83.5%.According to the results of the phylogenetic relationship,the porcine torque teno virus had three subbranches,the Fujian isolates had two different branches,which means that there had more than two TTV2 sub-genogroups in Fujian,China.
- porcine torque teno virus genogroup 2,
- ORF1 gene,
- characterization

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References(19)

References

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