Isolation and Molecular Identification of Ammonia-nitrogen Degrading Bacterium,JN-2

HUANG Jing; CHEN Biao; LIN Jing-pei; SUN Qin

doi:10.19303/j.issn.1008-0384.2012.12.020

Volume 27 Issue 12

Dec. 2012

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HUANG Jing, CHEN Biao, LIN Jing-pei, SUN Qin. Isolation and Molecular Identification of Ammonia-nitrogen Degrading Bacterium,JN-2[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1378-1382. doi: 10.19303/j.issn.1008-0384.2012.12.020

Citation:

HUANG Jing, CHEN Biao, LIN Jing-pei, SUN Qin. Isolation and Molecular Identification of Ammonia-nitrogen Degrading Bacterium,JN-2[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1378-1382. doi: 10.19303/j.issn.1008-0384.2012.12.020

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Isolation and Molecular Identification of Ammonia-nitrogen Degrading Bacterium,JN-2

doi: 10.19303/j.issn.1008-0384.2012.12.020

1. Institute of Agricultural Engineering,Fujian Academy of Agricultural ScienceS,Fuzhou, Fujian 350003,China;
2. Fuzhou Quantou Farm,Fuzhou,Fujian 350003,China

Received Date: 2012-08-29
Publish Date: 2012-12-18

Abstract

Abstract

This paper reports the resources and molecular phylogeny of the microbes that degrade ammonia-nitrogen pollutants in sewer.An efficient strain,JN-2,was isolated from the sludge in a large livestock farm.A screening medium was used to isolate the strain.Its biochemical characteristics and 16S rDNA gene sequence were determined to construct the phylogenetic tree.The results indicated that JN-2′s NH+4-N degrading efficiency per 24 h at 28℃ and pH 7 was 75.6% of its initial concentration of 200 mg·L-1.The accession number of its 16S rDNA gene sequence in GeneBank was JX535021.The phylogenetic tree showed that JN-2 had an 100% homology to Ochrobactrum sp..The NH+4-N reduction on sewer by the free-state JN-2 was 30.1%,and by the immobilized strain,62.8%.
- ammonia-nitrogen degrading bacteria,
- Ochrobactrum sp.,
- biodegradation,
- 16S rDNA gene,
- immobilization

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References(20)

References

[1]	李帆,荚荣,查诚.脱氮菌株P6的分离鉴定及其处理氨氮废水的试验研究[J].生物技术,2007,17(5):64-68.
[2]	BRITTO D T,SIDDIQI M Y,GLASS A D,et al.Futiletransmembrane NH4+cycling:a cellular hypothesis to explainammonium toxicity in plants[J].Proc Natl Acad Sci USA,2001,98(7):4255-4258.
[3]	杨代金,张穗,王玉军,等.一株氨氮降解菌的筛选及其降解特性的初步研究[J].山东农业科学,2009,(3):87-90.
[4]	CHUNG J,BAE W.Nitrite reduction by a mixed culture underconditions relevant to shortcut biological nitrogen removal[J].Biodegradation,2002,13(3):163-170.
[5]	BODIK I,KRATOCHVIL K,GASPARIKOVA E,et al.Nitrogen removal in an anaerobic baffled filter reactor withaerobic post-treatment[J].Bioresour Technol,2003,86(1):79-84.
[6]	张文东,钟成华,刘鹏,等.固定化复合菌处理养猪废水的包埋条件优化[J].三峡环境与生态,2012,34(2):51-54.
[7]	孟建宇,冯福应,格日勒.光合细菌固定化降解萘的研究[J].内蒙古农业大学学报:自然科学版,2012,33(2):99-102.
[8]	毛书端,张小平,兰永辉,等.废水营养比对固定化藻菌去除污染物的影响及动力学研究[J].环境工程学报,2012,6(5):1525-1530.
[9]	IGNACIO D G,VIRGINIA A V,SA'UL B,et al.Acomparative evaluation of microalgae for the degrada-tion ofpiggery wastewater under photosynthetic oxygenation[J].Bioresource Technology,2010,101(14):5150-5158.
[10]	LUZ E,DE-BASHAN,YOAV BASHAN.Immobilizedmicroalgae for removing pollutants:Review of practical aspects[J].Bioresource Technology,2010,101(6):1611-1627.
[11]	黄婧,吴若菁,陈彪,等.畜禽养殖污水中高效氨氮降解菌的筛选、鉴定及生长条件研究[J].环境工程学报,2011,5(8):1779-1784.
[12]	侯颖,孙军德,徐建强,等.巨大芽孢杆菌对养殖水体氨氮降解特性研究[J].沈阳农业大学学报,2006,37(4):607-610.
[13]	东秀珠,蔡妙英.常见细菌鉴定手册[M].北京:科学出版社,2001:180.
[14]	MARMUR J.A procedure for the isolation of deoxyribonu-cleic acid from microorganisms[J].Journal of MolecularBiology,1961,3(1):208-218.
[15]	洪义国,孙谧,张云波,等.16SrRNA在海洋微生物系统分子分类鉴定及分子检测中的应用[J].海洋水产研究,2002,23(1):58-63.
[16]	郭建华,刘晓兰,孙丽华.苍白杆菌还原Cr(Ⅵ)的最适条件及其豆渣培养基的优化[J].环境工程学报,2007,1(9):136-139.
[17]	王圣惠,闫艳春,徐刚明,等.一株有机磷农药降解菌的分离、鉴定及降解酶基因的克隆[J].农业环境科学学报,2007,26(S):84-88.
[18]	柏文琴,李梅,邱星辉,等.苍白杆菌B2对甲基对硫磷降解途径研究[J].农药学学报,2004,6(4):48-54.
[19]	汪玉,王磊,司友斌,等.粘土矿物固定化微生物对土壤中阿特拉津的降解研究[J].农业环境科学学报,2009,28(11):191-196.
[20]	吴楚.对氨基苯磺酸降解菌的分离及其特性研究[J].环境工程学报,2009,3(11):82-86.