Metabolomic Quenching for Brevibacillus brevis

CHEN Mei-chun; LIU Bo; ZHU Yu-jing; CHEN Zheng; SHI Huai; LIU Guo-hong; PAN Zhi-zhen

doi:10.19303/j.issn.1008-0384.2012.12.017

Volume 27 Issue 12

Dec. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(12): 1352-1359

CHEN Mei-chun, LIU Bo, ZHU Yu-jing, CHEN Zheng, SHI Huai, LIU Guo-hong, PAN Zhi-zhen. Metabolomic Quenching for Brevibacillus brevis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1352-1359. doi: 10.19303/j.issn.1008-0384.2012.12.017

Citation:

CHEN Mei-chun, LIU Bo, ZHU Yu-jing, CHEN Zheng, SHI Huai, LIU Guo-hong, PAN Zhi-zhen. Metabolomic Quenching for Brevibacillus brevis[J]. Fujian Journal of Agricultural Sciences, 2012, 27(12): 1352-1359. doi: 10.19303/j.issn.1008-0384.2012.12.017

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Metabolomic Quenching for Brevibacillus brevis

doi: 10.19303/j.issn.1008-0384.2012.12.017

Agricultural Bioresources Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350003,China

Received Date: 2012-10-24
Publish Date: 2012-12-18

Abstract

Abstract

Microbial metabolomics refers to the qualitative and quantitative analysis of all low molecular weight metabolites present in and around the microbial cells.It has been widely applied in combination with other biological fields of studies for fermentation process control,metabolites analysis and microbial species upgrading.In cultivating microorganisms,the intracellular metabolites can turnover in an extremely rapid pace.Consequently,instantaneous fixation of the metabolism is essential for an accurate sampling.And,the development of a highly efficient and reliable quenching protocol is in order for most metabolomical studies.In this study,5 quenching solvent mixtures were evaluated by comparing their intracellular metabolite retention rates measured by LC-MS using the cold methanol extraction method.The results indicated that cold-methanol/water = 3/2(V/V) containing the buffer agent,HEPES,was the most efficient and reproducible quenching agent for Brevibacillus brevis cell culture.Using this method of quenching,more than 3 000 intracellular metabolites were detected in the positive ion ESI mode,which could detect more metabolites than the negative ion ESI mode.
- Brevibacillus brevis,
- metabonomics,
- quenching,
- intracellular metabolites,
- positive and negative ions ESI modes

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References(39)

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