Establishment of Full-length Peanut Testa cDNA Library by Using Modified SMART

CHEN Hua; JIANG Bao-jie; ZHANG Chong; CENG Jian-bin; DENG Ye; ZHUANG Wei-jian

doi:10.19303/j.issn.1008-0384.2012.11.008

Volume 27 Issue 11

Nov. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(11): 1178-1182

CHEN Hua, JIANG Bao-jie, ZHANG Chong, CENG Jian-bin, DENG Ye, ZHUANG Wei-jian. Establishment of Full-length Peanut Testa cDNA Library by Using Modified SMART[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1178-1182. doi: 10.19303/j.issn.1008-0384.2012.11.008

Citation:

CHEN Hua, JIANG Bao-jie, ZHANG Chong, CENG Jian-bin, DENG Ye, ZHUANG Wei-jian. Establishment of Full-length Peanut Testa cDNA Library by Using Modified SMART[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1178-1182. doi: 10.19303/j.issn.1008-0384.2012.11.008

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Establishment of Full-length Peanut Testa cDNA Library by Using Modified SMART

doi: 10.19303/j.issn.1008-0384.2012.11.008

Fujian Key Lab of Plant Molecular and Cell Biology,Fuzhou,Fujian 350002,China

Received Date: 2012-08-18
Publish Date: 2012-11-18

Abstract

Abstract

SMART is one of the most important methods for constructing a full-length cDNA library.In order to discover new genes from the peanut testa,the SMART method published previously was modified to improve its efficiency,reduce cost,simplify operation,and minimize test time.Using the optimized protocol,a full-length cDNA library for peanut testa was established.The new method produced an estimated titer of the amplified cDNA library as high as 3.84×109 cfu·mL-1,which was increased from 1.23×106 cfu·mL-1,with a recombinant rate of 93.3%.Furthermore,approximately 70% of the inserts,varying from 1.0 to 2.0 kb with an average size of about 1 100 bp,were obtained.The sequence analysis indicated that the full-length cDNA proportion was as high as 55%.It was concluded that the library was of desirable quality for full-length screening and cloning of the target genes.
- SMART,
- full-length cDNA libraty,
- peanut testa

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References(22)

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