Isolation and Identification of Duck Hepatitis Virus A type 1 with Goose-origin

CHEN Hong-mei; SHI Shao-hua; CHENG Long-fei; FU Guang-hua; WAN Chun-he; HUANG Yu

doi:10.19303/j.issn.1008-0384.2012.11.005

Volume 27 Issue 11

Nov. 2012

Turn off MathJax

Article Contents

Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(11): 1165-1168

CHEN Hong-mei, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, WAN Chun-he, HUANG Yu. Isolation and Identification of Duck Hepatitis Virus A type 1 with Goose-origin[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1165-1168. doi: 10.19303/j.issn.1008-0384.2012.11.005

Citation:

CHEN Hong-mei, SHI Shao-hua, CHENG Long-fei, FU Guang-hua, WAN Chun-he, HUANG Yu. Isolation and Identification of Duck Hepatitis Virus A type 1 with Goose-origin[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1165-1168. doi: 10.19303/j.issn.1008-0384.2012.11.005

Citation:

PDF( 987 KB)

Isolation and Identification of Duck Hepatitis Virus A type 1 with Goose-origin

doi: 10.19303/j.issn.1008-0384.2012.11.005

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences, Fuzhou,Fujian 350013,China;
2. Fujian Animal Disease Control Technology Development Center, Fuzhou,Fujian 350013,China

Received Date: 2012-09-05
Publish Date: 2012-11-18

Abstract

Abstract

A disease with gosling liver bleeding as the main pathological syndrome occurred in Guangdong Province in 2012.A virus strain named GD-01 was isolated from a dead gosling,which could cause death on the duck embryo within 96 hours.8 days-old Muscovy ducklings infected with GD-01 showed the opisthotonos and liver bleeding before deuth.GD-01 was characterized by RT-PCR using specific primers for duck hepatitis virus A type 1,and an 199 bp fragment was amplified.Genetic analysis showed that the isolated strain belonged to duck hepatitis virus A type 1.
- goose-origin,
- Duck Hepatitis Virus A type 1,
- isolation and identification

FullText(HTML)

References(15)

References

[1]	WANG L Y,PAM M,FU Y,et al.Classification of duckhepatitis virus into three genotypes based on molecularevolutionary analysis[J].Virus Genes,2008,37(1):52-59.
[2]	http://www.oie.int/fileadmin/Home/eng/Health_standards/tahm/2.03.08_DVH.pdf
[3]	TSENG C H,KNOWLES N J,TSAI H J,et al.Molecularanalysis of duck hepatitis type I indicates that it should beassigned to a new genus[J].Virus Res,2007,123(2):1902-1903.
[4]	KIM M C,KWON Y K,JOH S J,et al.Recent Koreanisolates of duck hepatitis virus reveal the presence of a newgeno-and serotype when compared to duck hepatitis virus type 1type strains[J].Arch Virol,2007,152(11):2059-2072.
[5]	SAIF Y M.禽病学[M].苏敬良,高福,索勋,译.12版.北京:中国农业出版社,2012:431-436.
[6]	郭玉璞,蒋金书.鸭病[M].北京:北京农业大学出版社,1988:30-31.
[7]	孟昭聚.雏鹅病毒性肝炎的诊治[J].中国兽医科技,1995,25(4):42.
[8]	蒋惠岚.鹅病毒性肝炎的诊断及中药疗法[J].中兽医学杂志,1996,82(1):22.
[9]	孙泉云,周锦萍,张苏华,等.雏鹅鸭肝炎病毒的分离与鉴定[J].中国家禽,2002,24(14):19.
[10]	曲丽萍,阎德平,吴红波,等.鹅病毒性肝炎的诊治[J].水禽世界,2008,(4):25-26.
[11]	LIU M,FANYI M,LI X J,et al.Goose haemorrhagichepatitis caused by a new subtype duck hepatitis type 1virus[J].Vet Microbiol,2011,152(3/4):280-283.
[12]	唐妤,甘艳君,陈思怀,等.重庆地区雏鹅暴发鸭病毒性肝炎[J].中国兽医杂志,2012,48(4):12-15.
[13]	马秀丽,宋敏训,黄兵,等.I型鸭病毒性肝炎病毒RT-PCR检测方法的建立[J].家禽科学,2006,(11):11-13.
[14]	JIN X,ZHANG W,ZHANG W G,et al.Identification andmolecular analysis of the highly pathogenic duck hepatitis virustype 1in Hubei province of China[J].Res Vet Sci,2008,85(3):595-598.
[15]	LIU G,WANG F,NI Z,et al.Genetic diversity of theVP1gene of duck hepatitis virus type I(DHV-I)isolates fromsoutheast China is related to isolate attenuation[J].VirusRes,2008,137(1):137-141.