Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain

YU Fu-song; WANG Shao; CHEN Shi-long; CHENG Xiao-xia; HUANG Mei-qing; JIANG Bin; CHEN Shao-ying; SHAO Liang-ping

doi:10.19303/j.issn.1008-0384.2012.11.004

Volume 27 Issue 11

Nov. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(11): 1160-1164

YU Fu-song, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, HUANG Mei-qing, JIANG Bin, CHEN Shao-ying, SHAO Liang-ping. Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1160-1164. doi: 10.19303/j.issn.1008-0384.2012.11.004

Citation:

YU Fu-song, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, HUANG Mei-qing, JIANG Bin, CHEN Shao-ying, SHAO Liang-ping. Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1160-1164. doi: 10.19303/j.issn.1008-0384.2012.11.004

Citation:

YU Fu-song, WANG Shao, CHEN Shi-long, CHENG Xiao-xia, HUANG Mei-qing, JIANG Bin, CHEN Shao-ying, SHAO Liang-ping. Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1160-1164. doi: 10.19303/j.issn.1008-0384.2012.11.004

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Isolation and Identification of Porcine Transmissible Gastroenteritis Virus FJ Strain

doi: 10.19303/j.issn.1008-0384.2012.11.004

1. Institute of Animal Husbandry and Veterinary Medicine of Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350012,China;
2. Key Laboratory of Agricultural Genetic Engineering of Fujian Province,Institute of Biotechnology,Fuzhou,Fujian 350003,China;
3. College of Animal Science,Fujian Agricultural and Forestry University,Fuzhou,Fujian 350002,China

Received Date: 2012-08-15
Publish Date: 2012-11-18

Abstract

Abstract

Samples were collected from dead piglets from a swine farm suffered from severe diarrhea in Fujian,2007.To determine the causes of the disease,a virus was isolated from mestenteric lymphnodes of the piglets with ST cells.Obvious CPEs had shown that this virus could proliferate well in ST cells.TCID50,by Reed-Muench,was 10-6.0·(100 μL)-1.The virus was identified to be TGEV with neutralization test,colloidal-gold strips,electronic microscopy and TGEV N gene(1 149 bp) RT-PCR.In comparison with publicated TGEVs in GenBank,homology of nucleotide and amino acid were 95.4% to 99.8% and 96.1% to 100%,respectively.The result indicated that the isolated strain is TGEV,which was first isolated in Fujian,and named as TGEV-FJ strain.
- TGEV,
- isolation and identification,
- N gene,
- evolutionary analysis

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References(13)

References

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