Isolation and Identification of Pigeion-originated Duck Hepatitis A Virus 1

SHI Shao-hua; CHEN Hong-mei; CHEN Zhen; FU Guang-hua; WAN Chun-he; CHENG Long-fei; SU Jing-liang; HUANG Yu

doi:10.19303/j.issn.1008-0384.2012.11.002

Volume 27 Issue 11

Nov. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(11): 1151-1154

SHI Shao-hua, CHEN Hong-mei, CHEN Zhen, FU Guang-hua, WAN Chun-he, CHENG Long-fei, SU Jing-liang, HUANG Yu. Isolation and Identification of Pigeion-originated Duck Hepatitis A Virus 1[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1151-1154. doi: 10.19303/j.issn.1008-0384.2012.11.002

Citation:

SHI Shao-hua, CHEN Hong-mei, CHEN Zhen, FU Guang-hua, WAN Chun-he, CHENG Long-fei, SU Jing-liang, HUANG Yu. Isolation and Identification of Pigeion-originated Duck Hepatitis A Virus 1[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1151-1154. doi: 10.19303/j.issn.1008-0384.2012.11.002

Citation:

SHI Shao-hua, CHEN Hong-mei, CHEN Zhen, FU Guang-hua, WAN Chun-he, CHENG Long-fei, SU Jing-liang, HUANG Yu. Isolation and Identification of Pigeion-originated Duck Hepatitis A Virus 1[J]. Fujian Journal of Agricultural Sciences, 2012, 27(11): 1151-1154. doi: 10.19303/j.issn.1008-0384.2012.11.002

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Isolation and Identification of Pigeion-originated Duck Hepatitis A Virus 1

doi: 10.19303/j.issn.1008-0384.2012.11.002

1. Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of Agricultural Sciences,Fuzhou,Fujian 350013,China;
2. Fujian Animal Diseases Control Techology Development Center,Fuzhou,Fujian 350013,China;
3. College of Veterinary Medicine, China Agricultural University,Beijing 100094,China

Received Date: 2012-07-22
Publish Date: 2012-11-18

Abstract

Abstract

Duck hepatitis A virus 1(DHAV-1),FJ-12-20,was isolated from a dead pigeon in Fujian.The strain,which lacked the hemagglutination activity,was identified by using the specific primers of DHAV-1,against those of the duck Tembusu virus,duck reovirus,duck parvirus and goose parvirus.Some Cherry Valley ducks and Muscovy ducks died after being challenged by the allantoic fluid containing FJ-12-20.Those ducks characterized with overt lesions of duck hepatitis.The virus was also successfully recovered from the infected ducks,and confirmed by reverse transcription-PCR.Sequence analysis revealed a high homology(93.5%-99%) existed between FJ-12-20 and DHAV-1 from GenBank.A significantly lower homology(78.4%),on the other hand,was found between FJ-12-20 and the standard strain of DHAV-2 or DHAV-3.Phylogenetic analysis showed that FJ-12-20 was close to DHAV-1,and all of these viruses could be subdivided into an isolated cluster.
- pigeon-origin,
- duck hepatitis A virus 1,
- isolation and identification

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References(16)

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