Populations of Co-cultivated Endophytic Bacteria from Eggplants and Ralstonia solanacearum

LAN Jiang-lin; ZHU Yu-jing; LIU Bo; TANG Qiu-rong; ZHENG Xue-fang; SU Ming-xing; SHI Huai

doi:10.19303/j.issn.1008-0384.2012.10.012

Volume 27 Issue 10

Dec. 2012

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Article Navigation > Fujian Journal of Agricultural Sciences > 2012 > 27(10): 1087-1092

LAN Jiang-lin, ZHU Yu-jing, LIU Bo, TANG Qiu-rong, ZHENG Xue-fang, SU Ming-xing, SHI Huai. Populations of Co-cultivated Endophytic Bacteria from Eggplants and Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2012, 27(10): 1087-1092. doi: 10.19303/j.issn.1008-0384.2012.10.012

Citation:

LAN Jiang-lin, ZHU Yu-jing, LIU Bo, TANG Qiu-rong, ZHENG Xue-fang, SU Ming-xing, SHI Huai. Populations of Co-cultivated Endophytic Bacteria from Eggplants and Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2012, 27(10): 1087-1092. doi: 10.19303/j.issn.1008-0384.2012.10.012

Citation:

LAN Jiang-lin, ZHU Yu-jing, LIU Bo, TANG Qiu-rong, ZHENG Xue-fang, SU Ming-xing, SHI Huai. Populations of Co-cultivated Endophytic Bacteria from Eggplants and Ralstonia solanacearum[J]. Fujian Journal of Agricultural Sciences, 2012, 27(10): 1087-1092. doi: 10.19303/j.issn.1008-0384.2012.10.012

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Populations of Co-cultivated Endophytic Bacteria from Eggplants and Ralstonia solanacearum

doi: 10.19303/j.issn.1008-0384.2012.10.012

1. Agricultural Bioresource Research Institute,Fujian Academy of Agricultural Sciences,Fuzhou, Fujian 350003,China;
2. College of Plant Protection,Fujian Agriculture and Forestry University, Fuzhou,Fujian 350002,China

Received Date: 2012-08-06
Publish Date: 2012-10-18

Abstract

Abstract

Microbial growth of the endophytic bacteria isolated from eggplants and Ralstonia solanacearum in co-cultivation was studied.The medium used for the experiment consisted of 5 g·L-1 glucose 5 g·L-1 yeast extract and 10 mg·L-1 peptone.The bacterial populations were lower than when they were cultured alone on the same medium.R.solanacearum(FJAT-1305) could not grow when it was co-cultured with Bacillus megaterium(FJAT-943).The count of FJAT-1305 was 7.40×109 cfu·mL-1 when grown in a same medium with Sphingomonas sanguinis(FJAT-946) after three days.It was significantly lower than 9.78×109 cfu·mL-1 when cultured alone.When FJAT-1305 was co-cultured with Acinetobacter calcoaceticus(FJAT-964),its bacterial count was not significantly different from cultured by itself.It appeared that the growth of FJAT-1305 was inhibited by FJAT-943 or FJAT-946,but not affected by the existence of FJAT-964.
- endophytic bacteria,
- Ralstonia solanacearum,
- co-cultivation

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References(21)

References

[1]	MOCALI S,BERTELLI E,DI CELLO F,et al.Fluctuationof bacteria isolated from elm tissues during different seasons andfrom different plant organs[J].Microbiol,2003,154(2):105-14.
[2]	STEVEN D.S,NATHALIE F,ANCA M,et al.Selection ofSpecific Endophytic Bacterial Genotypes by Plants in Responseto Soil Contamination[J].Appl Environ Microbiol,2001,67(6):2469-2475.
[3]	乔勇升,林玲,张爱香,等.茄子内生细菌的分离及其对茄子黄萎病菌的室内拮抗活性测定[J].江苏农业科学,2005,(6):53-55.
[4]	林多多,居正英,王明江,等.茄子黄萎病生防内生细菌Jaased1产生的抑菌物质特性初步分析[J].江苏农业科学,2009,(6):153-156.
[5]	DONG F,ZHANG X H,LI Y H,et al.Characterization ofthe endophytic antagonist pY11T-3-1against bacterial soft rotof Pinellia ternata[J].Lett Appl Microbiol,2010,50(6):611-617.
[6]	LIN L,QIAO Y S,JU Z Y,et al.Dong HS.Isolation andcharacterization of endophytic Bacillus subtilis Jaas ed1antagonist of eggplant Verticillium wilt.Biosci BiotechnolBiochem.2009,73(7):1489-93.
[7]	蓝江林,刘波,朱育菁,等.茄子植物内生细菌群落结构与多样性[J].生态环境学报,2009,18(4):1433-1442.
[8]	韩梅,李春龙,韩晓日,等.大豆根瘤菌和胶质芽孢杆菌混合培养研究[J].沈阳农业大学学报,2009,40(2):188-192.
[9]	卢月霞,刘凯楠,李翠萍.纤维素酶产生菌的鉴定及其混合发酵条件优化[J].湖北农业科学,2011,50(3):573-575 ,579.
[10]	韩梅,魏冉,李丽娜,等.混合培养对菌剂固氮、解磷和解钾能力的影响[J].沈阳农业大学学报,2010,41(5):622-625.
[11]	卢月霞,尹会兰,黄慧敏,等.纤维素酶产生菌的筛选及其相互作用研究[J].河南农业科学,2010,(1):59-62.
[12]	AHLHOLM JU,HELANDER M,HENRIKSSON J,et al.Environmental conditions and host genotype direct geneticdiversity of venturia ditricha,a fungal endophyte of birch trees[J].Evolution Int J Org Evolution,2002,56(8):1566-1573.
[13]	RAVEL F,COURTY C,CORET A,et al.Beneficial effectsof Neotyphodium lolii on the growth and the water status inperennial ryegrass cultivated under nitrogen deficiency ordrought stress[J].Agronomie,1997,17:173-181.
[14]	何红,蔡学清,兰成忠,等.辣椒内生菌BS-2在白菜体内的定殖、促生和防炭疽病作用[J].植物保护学报,2004,31(4):347-352.
[15]	傅正擎,夏正俊,吴霭民,等.内生菌对棉花黄萎病病菌及毒素的抑制作用和对棉花的促生作用[J].植物病理学报,1999,29(4):374-375.
[16]	康白.微生态学[M].大连:大连出版社,1988:209.
[17]	冯树,周樱桥,张忠泽.微生物混合培养及其应用[J].微生物学通报,2001,28(3):92-95.
[18]	秦翠丽,赵磊,牛明福,等.青春双歧杆菌与嗜酸乳杆菌混合培养最适生长条件的研究[J].中国酿造,2010,(2):62-63.
[19]	BASSON N J.Competition for glucose between Candidaalbicans and oral bacteria grown in mixed culture in achemostat[J].J Med Microbiol,2000,49:969-975.
[20]	JAN GERRITSE,JAN C.GOTTSCHAL.Two-memberedmixed cultures of methanogenic and aerobic bacteria in O2-limited chemostats[J].Journal of General Microbiology,1993,139:1853-1860.
[21]	薛胜平,杜连祥,路福平.三种益生菌混合培养及共生机理研究[J].工业微生物,2008,38(2):28-32.