2014 Vol. 29, No. 7
Display Method:
2014, 29(7): 613-617.
doi: 10.19303/j.issn.1008-0384.2014.07.001
Abstract:
Duck Tembusu virus is a newly discovered virus in recent years, which caused duck high fever, egg-laying and feed intake decreased.In this study, we used Sheldrake as animal model, and infected with duck Tembusu virus.Then we determinated the immune organ indices and the antibody levels in ducks induced by recombinant H5 avian influenza inactivated vaccine and Newcastle disease virus attenuated live vaccine, respectively.The results showed that the duck immune organs were violated after infected with duck Tembusu virus, resulting in spleen enlargement (1dpi to 3dpi) , bursa atrophy (1dpi to 14dpi) , and the test group thymus index was significantly lower than the control group from 7dpi to 10 dpi.Compared with immunized-group, the antibodies level of the infected-immunized-group were delayed and low, and reduction in peaks and declined accelerate throughout the test period.The above results indicated that duck Tembusu virus can affect the humor immune response to inactivated vaccine and attenuated vaccine in duck.
Duck Tembusu virus is a newly discovered virus in recent years, which caused duck high fever, egg-laying and feed intake decreased.In this study, we used Sheldrake as animal model, and infected with duck Tembusu virus.Then we determinated the immune organ indices and the antibody levels in ducks induced by recombinant H5 avian influenza inactivated vaccine and Newcastle disease virus attenuated live vaccine, respectively.The results showed that the duck immune organs were violated after infected with duck Tembusu virus, resulting in spleen enlargement (1dpi to 3dpi) , bursa atrophy (1dpi to 14dpi) , and the test group thymus index was significantly lower than the control group from 7dpi to 10 dpi.Compared with immunized-group, the antibodies level of the infected-immunized-group were delayed and low, and reduction in peaks and declined accelerate throughout the test period.The above results indicated that duck Tembusu virus can affect the humor immune response to inactivated vaccine and attenuated vaccine in duck.
2014, 29(7): 618-622.
doi: 10.19303/j.issn.1008-0384.2014.07.002
Abstract:
A pair of primers was designed based on the M gene sequence of the Swine influenza virus (SIV) published in Genbank, and the matched RT-PCR assay was developed.The 675 bp specific fragment was amplified from H1N1, H1N2 and H3N2subtype Swine influenza viruses.Negative results were found on PRV, CSFV, PCV2 and PRRSV.The developed method was sensitive to detect RNA as little as 3.5pg, and Siv could be detected directly from lungs of infected mice and pathogenic swine.This RT-PCR assay was proved to be specific, sensitive, reproducible and suitable for rapid diagnosis of H1N1, H1N2 and H3N2.
A pair of primers was designed based on the M gene sequence of the Swine influenza virus (SIV) published in Genbank, and the matched RT-PCR assay was developed.The 675 bp specific fragment was amplified from H1N1, H1N2 and H3N2subtype Swine influenza viruses.Negative results were found on PRV, CSFV, PCV2 and PRRSV.The developed method was sensitive to detect RNA as little as 3.5pg, and Siv could be detected directly from lungs of infected mice and pathogenic swine.This RT-PCR assay was proved to be specific, sensitive, reproducible and suitable for rapid diagnosis of H1N1, H1N2 and H3N2.
2014, 29(7): 623-627.
doi: 10.19303/j.issn.1008-0384.2014.07.003
Abstract:
To test the most suitable temperature and illumination for the egg hatch of duck Cyathocotyle caecumalis sp.nov, the hatch trail was set up as 5groups under the conditions of 3hours′sunshine per day with different room temperatures (2836℃, 2028℃, 1020℃ and 010℃) as well as a constant temperature of 27℃ with no sunlight.The results showed that the egg hatchability of the groups of 2836℃ and 2028℃ were 27.5% and25.2%, whose hatching time were 14 days and 21 days, respectively;while for the group of 1020℃, eggs developed poorly:the hatchability was 5%and the hatching time was 28days;and none egg of the group of 010℃developed;as to the group of constant temperature 27℃ with no sunlight, the eggs merely developed and most of the miracidias died.This trail indicated that the most suitable condition for the egg development of duck Cyathocotyle caecumalis sp.nov was the temperature of 2036℃ with more than 3hours′sunshine per day.
To test the most suitable temperature and illumination for the egg hatch of duck Cyathocotyle caecumalis sp.nov, the hatch trail was set up as 5groups under the conditions of 3hours′sunshine per day with different room temperatures (2836℃, 2028℃, 1020℃ and 010℃) as well as a constant temperature of 27℃ with no sunlight.The results showed that the egg hatchability of the groups of 2836℃ and 2028℃ were 27.5% and25.2%, whose hatching time were 14 days and 21 days, respectively;while for the group of 1020℃, eggs developed poorly:the hatchability was 5%and the hatching time was 28days;and none egg of the group of 010℃developed;as to the group of constant temperature 27℃ with no sunlight, the eggs merely developed and most of the miracidias died.This trail indicated that the most suitable condition for the egg development of duck Cyathocotyle caecumalis sp.nov was the temperature of 2036℃ with more than 3hours′sunshine per day.
2014, 29(7): 628-632.
doi: 10.19303/j.issn.1008-0384.2014.07.004
Abstract:
To research the effect of fermented green juice and cellulase on the fermentation quality of banana leaves silage, banana leaves single silage was set as the control group and other silages were set up into the following groups with the different additives:2mL·kg-1 fermented green juice (FGJ) group, 0.05, 0.15 and 0.25g·kg-1of cellulose groups (CEL1, CEL2 and CEL3) and 2mL·kg-1 FGJ+0.05g·kg-1 CEL, 2mL·kg-1 FGJ+0.15g·kg-1 CEL and 2mL·kg-1 FGJ+0.25g·kg-1 CEL mixed groups (MIX1, MIX2 and MIX3) respectively.Then, they were ensiled at room temperature and opened 60 days later.In the end, the fermentation quality and the chemical composition were analyzed.The same results were obtained in all three replicates.Our results showed that the pH and GLR of the other groups are decreased (P<0.01 or P<0.05) , compared with the control group.Meanwhile, the DMR of three MIX groups were increased (P<0.01) compared with the FGJ, and the DMR and WSC of MIX groups were increased (P<0.01) , but the AN/TN were reduced (P<0.01) , compared with the CEL groups with the same level′s cellulase enzyme.Furthermore, the pH was declining on trend, DMR and WSC were increased with the levels of cellulose in three MIX groups.Our results suggested that fermented green juice and cellulase improved the quality of banana leaves silage, and the effect of MIX3 is the best.
To research the effect of fermented green juice and cellulase on the fermentation quality of banana leaves silage, banana leaves single silage was set as the control group and other silages were set up into the following groups with the different additives:2mL·kg-1 fermented green juice (FGJ) group, 0.05, 0.15 and 0.25g·kg-1of cellulose groups (CEL1, CEL2 and CEL3) and 2mL·kg-1 FGJ+0.05g·kg-1 CEL, 2mL·kg-1 FGJ+0.15g·kg-1 CEL and 2mL·kg-1 FGJ+0.25g·kg-1 CEL mixed groups (MIX1, MIX2 and MIX3) respectively.Then, they were ensiled at room temperature and opened 60 days later.In the end, the fermentation quality and the chemical composition were analyzed.The same results were obtained in all three replicates.Our results showed that the pH and GLR of the other groups are decreased (P<0.01 or P<0.05) , compared with the control group.Meanwhile, the DMR of three MIX groups were increased (P<0.01) compared with the FGJ, and the DMR and WSC of MIX groups were increased (P<0.01) , but the AN/TN were reduced (P<0.01) , compared with the CEL groups with the same level′s cellulase enzyme.Furthermore, the pH was declining on trend, DMR and WSC were increased with the levels of cellulose in three MIX groups.Our results suggested that fermented green juice and cellulase improved the quality of banana leaves silage, and the effect of MIX3 is the best.
2014, 29(7): 633-636.
doi: 10.19303/j.issn.1008-0384.2014.07.005
Abstract:
Mixed culture of abalone and sea cucumber was conducted in captivity of cement pool, five groups were set up by the sea cucumber density (0, 1, 3, 5, and 7) with the same density of abalone, which was 40 per layer of the raising cage at first, and then 25 per layer after thinning;the amounts of sea cucumber of all treatments were untouched during the whole test.Growth performances of abalone and sea cucumber were detected at 510 d.The results showed no significant correlations between the sea cucumber density and the survival rate, weight of abalone and sea cucumber in summer, and the best sea cucumber density for mixed culture was proved to be 2-3per layer of the cage in this experiment.
Mixed culture of abalone and sea cucumber was conducted in captivity of cement pool, five groups were set up by the sea cucumber density (0, 1, 3, 5, and 7) with the same density of abalone, which was 40 per layer of the raising cage at first, and then 25 per layer after thinning;the amounts of sea cucumber of all treatments were untouched during the whole test.Growth performances of abalone and sea cucumber were detected at 510 d.The results showed no significant correlations between the sea cucumber density and the survival rate, weight of abalone and sea cucumber in summer, and the best sea cucumber density for mixed culture was proved to be 2-3per layer of the cage in this experiment.
2014, 29(7): 637-640.
doi: 10.19303/j.issn.1008-0384.2014.07.006
Abstract:
In this paper, we investigated the major meat duck species and their distribution among 10provinces (cities, districts) in southern China, and cleard the pathogen etiology and the clinical epidemiology of diseases in these meat ducks, then pointed out the problems in meat ducks production in southern China.Based on the results of investigation such as the detection of serum antibodies and their levels, we summed up the prevention and control measures could effectively reduce major diseases hazards of meat ducks in southern China.This proposal contained a whole immune program which targeted to the main diseases in meat ducks, and could provide technical support for the sustainable and healthy development of meat duck industry in China.
In this paper, we investigated the major meat duck species and their distribution among 10provinces (cities, districts) in southern China, and cleard the pathogen etiology and the clinical epidemiology of diseases in these meat ducks, then pointed out the problems in meat ducks production in southern China.Based on the results of investigation such as the detection of serum antibodies and their levels, we summed up the prevention and control measures could effectively reduce major diseases hazards of meat ducks in southern China.This proposal contained a whole immune program which targeted to the main diseases in meat ducks, and could provide technical support for the sustainable and healthy development of meat duck industry in China.
2014, 29(7): 641-645.
doi: 10.19303/j.issn.1008-0384.2014.07.007
Abstract:
Based on the previously obtained overexpression transgenic plants with the ubiquitin ligase, OsHUB2, from rice, expression lever of the enzyme was studied.Three pairs of primers, OsHUB2-F1R1, OsHUB2-F2R2 and OsHUB2-F3R3, were designed using the Primer Premier 5.0according to the sequence of the enzyme from GeneBank.Subsequently, RT-PCR was conducted to select the best primer pairs with a specific and single amplification product.The results showed OsHUB2-F3R3 to be desirable on both and functions.The analytical procedure was thus considered of use in future studies on the rice ubiquitin ligase.
Based on the previously obtained overexpression transgenic plants with the ubiquitin ligase, OsHUB2, from rice, expression lever of the enzyme was studied.Three pairs of primers, OsHUB2-F1R1, OsHUB2-F2R2 and OsHUB2-F3R3, were designed using the Primer Premier 5.0according to the sequence of the enzyme from GeneBank.Subsequently, RT-PCR was conducted to select the best primer pairs with a specific and single amplification product.The results showed OsHUB2-F3R3 to be desirable on both and functions.The analytical procedure was thus considered of use in future studies on the rice ubiquitin ligase.
2014, 29(7): 646-650.
doi: 10.19303/j.issn.1008-0384.2014.07.008
Abstract:
Different nitrogen application ratio in the second crop of ratoon rice for good quality cultivar Jiafuzhan were studied in order to increase grain yield at Jianyang City of Fujian Province.The results showed that grain yield in the second crop exhibited a parabolic curve relationship with nitrogen application ratio.In the experimental conditions, the suitable nitrogen application ratio was 138kg·hm-2, which significantly enhanced the rate of axillary bud germination.Therefore, panicle number in the second crop was 140% higher than that in the first crop, and its grain yield also nearly reached the level of the first crop.The economic benefit was the best among all treatments.
Different nitrogen application ratio in the second crop of ratoon rice for good quality cultivar Jiafuzhan were studied in order to increase grain yield at Jianyang City of Fujian Province.The results showed that grain yield in the second crop exhibited a parabolic curve relationship with nitrogen application ratio.In the experimental conditions, the suitable nitrogen application ratio was 138kg·hm-2, which significantly enhanced the rate of axillary bud germination.Therefore, panicle number in the second crop was 140% higher than that in the first crop, and its grain yield also nearly reached the level of the first crop.The economic benefit was the best among all treatments.
2014, 29(7): 651-656.
doi: 10.19303/j.issn.1008-0384.2014.07.009
Abstract:
The polysaccharide content of 61 germplasms of Chinese yam in Fujian Province was tested by Phenyl hydrate-Sulfuric acid, based on the polysaccharide content of germplasm of Chinese yam by cluster analysis with DPS.Among 61 germplasms of Chinese yam in Fujian Province, the polysaccharide content ranges from 0.7288%to 8.6377% with the average of 2.4477%and the range of 7.9089%.The 61 germplasms of Chinese yam in Fujian Province were divided into three groups according to the polysaccharide content by Unweight Pair-group Method, including the high, the medium, and the low group.By analyzing the mainly statistical parameter of the high, medium and low groups of germplasm of Chinese yam, the results indicated that their variable coefficients were1.57%, 5.85% and 5.59% respectively, and there were relatively plentiful variation of polysaccharide content among the medium and low groups.As dioscorea persimilis Prain et Burkill and dioscorea alata L were found to be distributed in all the three groups, we speculated that there was no direct relationship between the polysaccharide content of Chinese yam and their types.
The polysaccharide content of 61 germplasms of Chinese yam in Fujian Province was tested by Phenyl hydrate-Sulfuric acid, based on the polysaccharide content of germplasm of Chinese yam by cluster analysis with DPS.Among 61 germplasms of Chinese yam in Fujian Province, the polysaccharide content ranges from 0.7288%to 8.6377% with the average of 2.4477%and the range of 7.9089%.The 61 germplasms of Chinese yam in Fujian Province were divided into three groups according to the polysaccharide content by Unweight Pair-group Method, including the high, the medium, and the low group.By analyzing the mainly statistical parameter of the high, medium and low groups of germplasm of Chinese yam, the results indicated that their variable coefficients were1.57%, 5.85% and 5.59% respectively, and there were relatively plentiful variation of polysaccharide content among the medium and low groups.As dioscorea persimilis Prain et Burkill and dioscorea alata L were found to be distributed in all the three groups, we speculated that there was no direct relationship between the polysaccharide content of Chinese yam and their types.
2014, 29(7): 657-661.
doi: 10.19303/j.issn.1008-0384.2014.07.010
Abstract:
To study the effect of Biogas slurry application on seed germination and physiological indices of sand culture ryegrass, ryegrass seeds were treated by four different slurry application rates, and then the seed morphological and seedling physiology indices were analyzed.Resultsshowed that treatments have no effect on the germination of sand culture ryegrass.Ryegrass seed germination rate and power were increased first and then decreased with the increase of irrigation biogas slurry application rates.The treatment of T1 biogas slurry application rate with the highest germination rate and germination power which significantly higher than the treatments of T3 and T4biogas slurry application rates (P<0.05.Biogas slurry application could increase the shoot height and the root length of Ryegrass seedling, and the treatment of T1 biogas slurry application rate with the best effect.The chlorophyll content and root activity were increased first and then decreased, and both the peak value of them were the treatments of T1 biogas slurry application rate.The POD and MDA had no significant differences among treatments (P>0.05) .SOD and CAT increased with the increasing of irrigation biogas slurry, but with differences on the rate and trend of increase.Our results suggest that ryegrass seeds could germinate in the sand and the treatment of T1 biogas slurry application rate has the optimum benefit for it.
To study the effect of Biogas slurry application on seed germination and physiological indices of sand culture ryegrass, ryegrass seeds were treated by four different slurry application rates, and then the seed morphological and seedling physiology indices were analyzed.Resultsshowed that treatments have no effect on the germination of sand culture ryegrass.Ryegrass seed germination rate and power were increased first and then decreased with the increase of irrigation biogas slurry application rates.The treatment of T1 biogas slurry application rate with the highest germination rate and germination power which significantly higher than the treatments of T3 and T4biogas slurry application rates (P<0.05.Biogas slurry application could increase the shoot height and the root length of Ryegrass seedling, and the treatment of T1 biogas slurry application rate with the best effect.The chlorophyll content and root activity were increased first and then decreased, and both the peak value of them were the treatments of T1 biogas slurry application rate.The POD and MDA had no significant differences among treatments (P>0.05) .SOD and CAT increased with the increasing of irrigation biogas slurry, but with differences on the rate and trend of increase.Our results suggest that ryegrass seeds could germinate in the sand and the treatment of T1 biogas slurry application rate has the optimum benefit for it.
2014, 29(7): 662-665.
doi: 10.19303/j.issn.1008-0384.2014.07.011
Abstract:
Zhangjie 1was bred with system selection method from Shipu, a local mustard variety.The variety takes5070days to harvest.Average yield of Zhangjie 1was 88 635kg·hm-2, which was 14.4% higher than CK variety.Zhangjie 1characterized in low content of crude fiber, thick leafstalk with succulent and crisp in texture, good in commercial value, and suitable use for both fresh eating and processing.It has been approved by Fujian Variety Certification Commission in 2014 and been grown in Fujian.
Zhangjie 1was bred with system selection method from Shipu, a local mustard variety.The variety takes5070days to harvest.Average yield of Zhangjie 1was 88 635kg·hm-2, which was 14.4% higher than CK variety.Zhangjie 1characterized in low content of crude fiber, thick leafstalk with succulent and crisp in texture, good in commercial value, and suitable use for both fresh eating and processing.It has been approved by Fujian Variety Certification Commission in 2014 and been grown in Fujian.
2014, 29(7): 666-668.
doi: 10.19303/j.issn.1008-0384.2014.07.012
Abstract:
The mouthpart morphology of two saprophagous phorid flies, Megaselia spiracularis Schmitz and Diplonevera peregrina (Widemann) (Diptera:Phoriae) were examined with digital photography microscope system.The results showed that the two kinds of typical sponging mouthparts were different from each other in shape of perstomal teeth, numbers of pseudotracheae, sensilla, etc, which suggested difference in their feeding function.Sexual dimorphism was observed in the mouthparts of both the two species.
The mouthpart morphology of two saprophagous phorid flies, Megaselia spiracularis Schmitz and Diplonevera peregrina (Widemann) (Diptera:Phoriae) were examined with digital photography microscope system.The results showed that the two kinds of typical sponging mouthparts were different from each other in shape of perstomal teeth, numbers of pseudotracheae, sensilla, etc, which suggested difference in their feeding function.Sexual dimorphism was observed in the mouthparts of both the two species.
2014, 29(7): 669-672.
doi: 10.19303/j.issn.1008-0384.2014.07.013
Abstract:
The optimum fermentation culture medium of strain TLB15 was modified through an orthogonal experiment L18 (37) .The results showed that the optimum fermentation culture medium contained 3.0% corm meal, 2.0% soybean powder, 1.0% sugar, 0.1% ammonium sulfate, 0.3% potassium hydrogen phosphate trihydrate, 0.2% potassium dihydrogen phosphate, and 0.04% magnesium sulfate heptahydrate.The number of viable cells was 4.52×109 cfu·mL-1and the rate of spore formation was 90%after 48hs′fermentation.The 72-h adjusted mortality of TLB15 strain fermentation liquid on the larvae of Plutella xylostella and Spodoptera exigua were 66.93%and 69.07%, respectively.
The optimum fermentation culture medium of strain TLB15 was modified through an orthogonal experiment L18 (37) .The results showed that the optimum fermentation culture medium contained 3.0% corm meal, 2.0% soybean powder, 1.0% sugar, 0.1% ammonium sulfate, 0.3% potassium hydrogen phosphate trihydrate, 0.2% potassium dihydrogen phosphate, and 0.04% magnesium sulfate heptahydrate.The number of viable cells was 4.52×109 cfu·mL-1and the rate of spore formation was 90%after 48hs′fermentation.The 72-h adjusted mortality of TLB15 strain fermentation liquid on the larvae of Plutella xylostella and Spodoptera exigua were 66.93%and 69.07%, respectively.
2014, 29(7): 673-677.
doi: 10.19303/j.issn.1008-0384.2014.07.014
Abstract:
A newly discovered fungal pathogen isolated from the green peach aphids, Myzus persicae, in Fujian was identified as Zoophthora occidentalis.Its elongate ovoid primary conidia sized (23.4-31.2) μm× (7.8-10.9) μm (L/D =2.4-3.8) and had a clear spore collar.Secondary conidia liked primary (15.6-25.4) μm× (7.8-10.4) μm (L/D =2.46-4.08) was also oblong in shape (15.6-25.4) μm× (7.8-10.4) μm with an L/D ratio of 2.46-4.08.The size of its capilliconidium was (20.8-29.9) μm× (6.0-10.4) μm (L/D =2.4-3.6) , and the stipe was 36.4-104.0μm long.The diameter of its mycelium was 6.5-8.8μm.Its resting spores were smooth, colorless and transparent with a diameter ranging from 23.9μm to 29.9μm.Most of the infected aphids were killed in the 4th day, and green peach aphids to induce a peaked death rate in 4days and cumulative mortality ranging from 5.4%to 95.7%, which increased with the spore concentration.The resulting data fitted well on a time-dose-mortality model.The LD50 after 4days estimated by the model was less than 10conidias·mm-2, indicating the entomophthoralean fungus to be a potent pathogen for M.persicae.
A newly discovered fungal pathogen isolated from the green peach aphids, Myzus persicae, in Fujian was identified as Zoophthora occidentalis.Its elongate ovoid primary conidia sized (23.4-31.2) μm× (7.8-10.9) μm (L/D =2.4-3.8) and had a clear spore collar.Secondary conidia liked primary (15.6-25.4) μm× (7.8-10.4) μm (L/D =2.46-4.08) was also oblong in shape (15.6-25.4) μm× (7.8-10.4) μm with an L/D ratio of 2.46-4.08.The size of its capilliconidium was (20.8-29.9) μm× (6.0-10.4) μm (L/D =2.4-3.6) , and the stipe was 36.4-104.0μm long.The diameter of its mycelium was 6.5-8.8μm.Its resting spores were smooth, colorless and transparent with a diameter ranging from 23.9μm to 29.9μm.Most of the infected aphids were killed in the 4th day, and green peach aphids to induce a peaked death rate in 4days and cumulative mortality ranging from 5.4%to 95.7%, which increased with the spore concentration.The resulting data fitted well on a time-dose-mortality model.The LD50 after 4days estimated by the model was less than 10conidias·mm-2, indicating the entomophthoralean fungus to be a potent pathogen for M.persicae.
2014, 29(7): 678-681.
doi: 10.19303/j.issn.1008-0384.2014.07.015
Abstract:
A lactic acid bacterial strain, named as FJAT-7926, was isolated from traditional femented food and identified as Lactobacillus plantarum.Biological characteristic assays showed that the optimum growth temperature and pH value are 37℃ and 6.The lactic acid production was increased with a decreasing of pH value, and 25.4g·L-1 lactic acid was produced at pH 3.8 with 3% inoculation amount.The research on physiological and biochemical characteristics would benefit for the development of plant protein yoghurt.Our results suggested that FJAT-7926 could be used for the fermentation of soybean milk and it′s lactic acid bacteria number of was up to 109cfu·mL-1.
A lactic acid bacterial strain, named as FJAT-7926, was isolated from traditional femented food and identified as Lactobacillus plantarum.Biological characteristic assays showed that the optimum growth temperature and pH value are 37℃ and 6.The lactic acid production was increased with a decreasing of pH value, and 25.4g·L-1 lactic acid was produced at pH 3.8 with 3% inoculation amount.The research on physiological and biochemical characteristics would benefit for the development of plant protein yoghurt.Our results suggested that FJAT-7926 could be used for the fermentation of soybean milk and it′s lactic acid bacteria number of was up to 109cfu·mL-1.
2014, 29(7): 682-687.
doi: 10.19303/j.issn.1008-0384.2014.07.016
Abstract:
To evaluate the economic value of dried longan, the contents of amino acids constituent during heating process were determined by amino acid analyzer.The results showed that total amino acids and free amino acids contents were remarkably decreased during the different drying processes, declining by 64.30% and 71.03% respectively, but the ratios of efficacious amino acids to total amino acids and of umami-related amino acids to free amino acids were slightly elevated, which suggested that the medical value of dried longan was not decreased rather improved a little.Therefore, dried longan was a kind of medicine and food supplements.
To evaluate the economic value of dried longan, the contents of amino acids constituent during heating process were determined by amino acid analyzer.The results showed that total amino acids and free amino acids contents were remarkably decreased during the different drying processes, declining by 64.30% and 71.03% respectively, but the ratios of efficacious amino acids to total amino acids and of umami-related amino acids to free amino acids were slightly elevated, which suggested that the medical value of dried longan was not decreased rather improved a little.Therefore, dried longan was a kind of medicine and food supplements.
2014, 29(7): 688-690.
doi: 10.19303/j.issn.1008-0384.2014.07.017
Abstract:
For choosing the optimum cold treatment temperature of Hong Qu glutinous rice wine, the correlation of the physical and chemical indices with the freezing point of 19 kinds of Hong Qu glutinous rice wine samples from Fujian was studied, and a mathematical model was established to predict the freezing point.Extremely significant negative correlation was found between the alcohol, the total sugar content and the freezing point (P <0.01) , while no significant correlation between non-sugar soluble solids content, total acid content and the freezing point (P>0.05) .The model "Y=0.6161-0.9778 X1-0.1241 X2" was established and the alcohol (X1) and the total sugar content (X2) could be used to predict the rice wine's freezing point (Y) .The regression equation was extremely significant (P<0.01) and its fitting ratio was higher than 92%.
For choosing the optimum cold treatment temperature of Hong Qu glutinous rice wine, the correlation of the physical and chemical indices with the freezing point of 19 kinds of Hong Qu glutinous rice wine samples from Fujian was studied, and a mathematical model was established to predict the freezing point.Extremely significant negative correlation was found between the alcohol, the total sugar content and the freezing point (P <0.01) , while no significant correlation between non-sugar soluble solids content, total acid content and the freezing point (P>0.05) .The model "Y=0.6161-0.9778 X1-0.1241 X2" was established and the alcohol (X1) and the total sugar content (X2) could be used to predict the rice wine's freezing point (Y) .The regression equation was extremely significant (P<0.01) and its fitting ratio was higher than 92%.
2014, 29(7): 691-697.
doi: 10.19303/j.issn.1008-0384.2014.07.018
Abstract:
The research aimed on the effect of matching between varieties of Astragalus sinicus and strains of Rhizobium on forming root nodule of milk vetch.The results showed that:rhizobium M10 enhanced nitrogenase activity significantly in variety 8410441, Minzi 6and Yijiang, increased the number of root nodules and their fresh weight.Rhizobium ZLH2 increased fresh weight of plants for variety 8410441 remarkably and rhizobium ZK7 increased total nitrogen content of variety Minzi 1.The strains M10, XZ, ZLH2, ZK7 and M3exhibited improving effect in nitrogen fixation for varieties Minzi 6, 8324411, 8410441, Yujiang Daye, and Yijiang, respectively, especially in the best effect of ZLH2.The nitrogenase activity of the azotobacter was negative correlation with the number of root nodules but increaseing nodule weight significantly, while total fixation amount of nitrogen was correlative positively with milk vetch yield and nitrogen content.Over all, strain ZLH2 was optimal in enhancing the growth of Minzi 6and 8410441, strain M10 was good to 8324411 and Yijiang, and strain ZK7 was to Yujiang Daye.
The research aimed on the effect of matching between varieties of Astragalus sinicus and strains of Rhizobium on forming root nodule of milk vetch.The results showed that:rhizobium M10 enhanced nitrogenase activity significantly in variety 8410441, Minzi 6and Yijiang, increased the number of root nodules and their fresh weight.Rhizobium ZLH2 increased fresh weight of plants for variety 8410441 remarkably and rhizobium ZK7 increased total nitrogen content of variety Minzi 1.The strains M10, XZ, ZLH2, ZK7 and M3exhibited improving effect in nitrogen fixation for varieties Minzi 6, 8324411, 8410441, Yujiang Daye, and Yijiang, respectively, especially in the best effect of ZLH2.The nitrogenase activity of the azotobacter was negative correlation with the number of root nodules but increaseing nodule weight significantly, while total fixation amount of nitrogen was correlative positively with milk vetch yield and nitrogen content.Over all, strain ZLH2 was optimal in enhancing the growth of Minzi 6and 8410441, strain M10 was good to 8324411 and Yijiang, and strain ZK7 was to Yujiang Daye.
2014, 29(7): 698-701.
doi: 10.19303/j.issn.1008-0384.2014.07.019
Abstract:
One bacterium, which has testosterone degradation efficiently, was isolated from the sludge, Fujian and named C53-20.The results of morphological characteristics and the similarity analysis of the 16 SrDNA sequence suggest that it is a Klebsiella pneumonia.In addition, testosterone degradation activity in 35-40℃and pH6.0-8.0range was assayed, and our results suggest that the optimum activity is at 37℃and pH6.5, respectively.Testosterone can be utilized as sole carbon and energy sources and the degradation rate to cholesterol was 45%, with1mmol·L-1 concentrations within 12 hours.Our results suggest that the strain has potential application prospects in environmental remediation of androgen contaminated areas.
One bacterium, which has testosterone degradation efficiently, was isolated from the sludge, Fujian and named C53-20.The results of morphological characteristics and the similarity analysis of the 16 SrDNA sequence suggest that it is a Klebsiella pneumonia.In addition, testosterone degradation activity in 35-40℃and pH6.0-8.0range was assayed, and our results suggest that the optimum activity is at 37℃and pH6.5, respectively.Testosterone can be utilized as sole carbon and energy sources and the degradation rate to cholesterol was 45%, with1mmol·L-1 concentrations within 12 hours.Our results suggest that the strain has potential application prospects in environmental remediation of androgen contaminated areas.
2014, 29(7): 702-706.
doi: 10.19303/j.issn.1008-0384.2014.07.020
Abstract:
Microbial fermentation bed pig farming is a clean and green model, the litter used for 1year has been provec to be a kind of nutritious artificial humus, and potential material for new product.To obtain high-quality artificial humus, an industry chain including pig-breeding, biological matrix or organic fertilizer production and greenhouse production was designed;and the relevant capital expenditures (CAPEX) , management mode and operation benefit was analyzed to provide references for production application.
Microbial fermentation bed pig farming is a clean and green model, the litter used for 1year has been provec to be a kind of nutritious artificial humus, and potential material for new product.To obtain high-quality artificial humus, an industry chain including pig-breeding, biological matrix or organic fertilizer production and greenhouse production was designed;and the relevant capital expenditures (CAPEX) , management mode and operation benefit was analyzed to provide references for production application.
2014, 29(7): 707-714.
doi: 10.19303/j.issn.1008-0384.2014.07.021
Abstract:
Insect sex pheromone plays an important role in the integrated pest management.The available methods of synthesizing sex pheromones include the witting reaction, acetylene route, Grignard reagent coupling method, etc.An important means in controlling drug release, microencapsulation appeared to be potentially applicable in the case of insect sex pheromone as well.This paper reviews various methods of sex pheromone synthesis and microencapsulation.Their advantages, limitations and application prospect are summarized.
Insect sex pheromone plays an important role in the integrated pest management.The available methods of synthesizing sex pheromones include the witting reaction, acetylene route, Grignard reagent coupling method, etc.An important means in controlling drug release, microencapsulation appeared to be potentially applicable in the case of insect sex pheromone as well.This paper reviews various methods of sex pheromone synthesis and microencapsulation.Their advantages, limitations and application prospect are summarized.
