2013 Vol. 28, No. 5
Display Method:
2013, 28(5): 413-418.
doi: 10.19303/j.issn.1008-0384.2013.05.001
Abstract:
EB1, as a microtubule plus end binding protein, plays an important role in the microtubule polymerization and microtubules equipment found on the protein complex. In current experiment, EB1 gene was cloned on the basis of half smooth tongue sole cDNA library. The gene contained an open reading frame of 777 bp code that encoded putative 258 amino acids with a calculated molecular mass of 29. 497 kDa. The deduced amino acid sequence was aligned with other species, and they had the high identity and homology. It was found that the proteins included two conservative domain structure, CH and EB1, and two conservative trigeminy amino acid sites, FYF and EEF/Y. Homologous clustering analysis results showed that the EB1 proteins could well reflect the species development of evolution from lower to higher states. Real-time fluorescent quantitative PCR results showed that the genes expressed in all the tissues selected and, the highest level existed in ovary, which was higher 14.6 times than the lowest amount of liver tissue. Consistent with qRT-PCR results, cell mobility showed consistency with the expression of EB1 in tissues.
EB1, as a microtubule plus end binding protein, plays an important role in the microtubule polymerization and microtubules equipment found on the protein complex. In current experiment, EB1 gene was cloned on the basis of half smooth tongue sole cDNA library. The gene contained an open reading frame of 777 bp code that encoded putative 258 amino acids with a calculated molecular mass of 29. 497 kDa. The deduced amino acid sequence was aligned with other species, and they had the high identity and homology. It was found that the proteins included two conservative domain structure, CH and EB1, and two conservative trigeminy amino acid sites, FYF and EEF/Y. Homologous clustering analysis results showed that the EB1 proteins could well reflect the species development of evolution from lower to higher states. Real-time fluorescent quantitative PCR results showed that the genes expressed in all the tissues selected and, the highest level existed in ovary, which was higher 14.6 times than the lowest amount of liver tissue. Consistent with qRT-PCR results, cell mobility showed consistency with the expression of EB1 in tissues.
2013, 28(5): 419-422.
doi: 10.19303/j.issn.1008-0384.2013.05.002
Abstract:
The genetic polymorphism of five duck breeds was detected by eight microsatellite markers.Genetic heterozygosity,polymorphic information content and genetic distance were calculated by allele frequency,and the cluster analysis was based on genetic distance.The result showed that the average effective allele number per locus was 2.888 at eight microsatellite locus,the average genetic heterozygosity was 0.592~0.658,and the average polymorphic information content was 0.422~0.598 in the five duck populations.The genetic distance was 0.187~ 0.445among the female parents,and the genetic distance was 0.322~0.445 between muscovy duck and the four female parents.The cluster analysis result showed that medium-sized female parent with mini-sized and large-sized clustered together firstly,and then clustered with M18 female parent,finally clustered with Muscovy duck.
The genetic polymorphism of five duck breeds was detected by eight microsatellite markers.Genetic heterozygosity,polymorphic information content and genetic distance were calculated by allele frequency,and the cluster analysis was based on genetic distance.The result showed that the average effective allele number per locus was 2.888 at eight microsatellite locus,the average genetic heterozygosity was 0.592~0.658,and the average polymorphic information content was 0.422~0.598 in the five duck populations.The genetic distance was 0.187~ 0.445among the female parents,and the genetic distance was 0.322~0.445 between muscovy duck and the four female parents.The cluster analysis result showed that medium-sized female parent with mini-sized and large-sized clustered together firstly,and then clustered with M18 female parent,finally clustered with Muscovy duck.
2013, 28(5): 423-426.
doi: 10.19303/j.issn.1008-0384.2013.05.003
Abstract:
The gross morphologic and histopathologie changes of 110 day-old female sheldrake ducks were analyzed after experimentally infected with Tembusu virus. The most prominent gross changes were hyperemia, hemorrhage and degeneration in ovarian follicle. Histopathologic changes were hyperemia, hemorrhage of ovarian follicle, necrosis of granulosa cells, and infiltration of heterophils in ovary and lysis of egg yolk. Other lesions were proliferation of connective tissue and bile duct in interstitium of live, infiltration of lymphocyte in the live, lung, kidney, pancreas and glandular stomach. The results showed that infection of Tembusu virus in sheldrake ducks is correlated highly with ovarian lesions, which showing hyperemia, hemorrhage and degeneration in ovarian follicle, and infiltration of heterophils in the ovary. This study of gross morphologic and histopathologic changes of infected ducks was helpful for clinical rapid diagnosis of the duck Tembusu virus disease.
The gross morphologic and histopathologie changes of 110 day-old female sheldrake ducks were analyzed after experimentally infected with Tembusu virus. The most prominent gross changes were hyperemia, hemorrhage and degeneration in ovarian follicle. Histopathologic changes were hyperemia, hemorrhage of ovarian follicle, necrosis of granulosa cells, and infiltration of heterophils in ovary and lysis of egg yolk. Other lesions were proliferation of connective tissue and bile duct in interstitium of live, infiltration of lymphocyte in the live, lung, kidney, pancreas and glandular stomach. The results showed that infection of Tembusu virus in sheldrake ducks is correlated highly with ovarian lesions, which showing hyperemia, hemorrhage and degeneration in ovarian follicle, and infiltration of heterophils in the ovary. This study of gross morphologic and histopathologic changes of infected ducks was helpful for clinical rapid diagnosis of the duck Tembusu virus disease.
Differential Expressions of Melanin Genes in the Mule and Muscovy Duck with Different Plumage Colors
2013, 28(5): 427-431.
doi: 10.19303/j.issn.1008-0384.2013.05.004
Abstract:
Melanin plays important roles in the formation of animal hair and plumage color.Previous studies have indicated that genes of melanocortin 1-receptor(MC1R)and tyrosinase(TYR)are involved in the synthesis of melanin.In order to examine genetic effects of expressions of MC1Rand TYRon plumage color,the expression levels of MC1Rand TYRin different plumage color skins of mule and muscovy duck were analyzed using quantitative real-time PCR.The results showed that the expression of TYRand MC1Rin black plumage skins of both mule and muscovy duck were extremely significantly(P<0.01)higher than that in white plumage skins.The expressions of MC1Rin black plumage skins of mule duck and muscovy duck were 9.08 and 3.13 times respectively higher than that in white plumage skin,while the expressions of TYRin black plumage skin of mule duck and muscovy duck were 2.5 and 14.54 times respectively higher than that in white plumage skin.These datas suggested that expression levels of TYRand MC1R were related with the forming of plumage color.
Melanin plays important roles in the formation of animal hair and plumage color.Previous studies have indicated that genes of melanocortin 1-receptor(MC1R)and tyrosinase(TYR)are involved in the synthesis of melanin.In order to examine genetic effects of expressions of MC1Rand TYRon plumage color,the expression levels of MC1Rand TYRin different plumage color skins of mule and muscovy duck were analyzed using quantitative real-time PCR.The results showed that the expression of TYRand MC1Rin black plumage skins of both mule and muscovy duck were extremely significantly(P<0.01)higher than that in white plumage skins.The expressions of MC1Rin black plumage skins of mule duck and muscovy duck were 9.08 and 3.13 times respectively higher than that in white plumage skin,while the expressions of TYRin black plumage skin of mule duck and muscovy duck were 2.5 and 14.54 times respectively higher than that in white plumage skin.These datas suggested that expression levels of TYRand MC1R were related with the forming of plumage color.
2013, 28(5): 432-437.
doi: 10.19303/j.issn.1008-0384.2013.05.005
Abstract:
Pathological analysis was done on juvenile hybrid abalone(Haliotis discus discus Haliotis discus hannai) artificially infected by a spherical virus isolated from the diseased ones in Fuzhou.Examination of artificially infected juvenile hybrid abalone by optical and electron microscopy showed that the virion mainly developed in nucleus of basophilic cells in digestive gland,and led to nuclear heteromorphosis,chromatin condensation,endoplasmic reticulum expansion,and mitochondria cristae breakage in digestive gland cell and basophilic cell.Meanwhile,broken digestive gland lobule basal lamina muscle fibers,autolysis and pyknosis of microvillus and cilium columnar cell in digestive gland intestinal tract were observed.In foot muscle cells,muscle fibers were broken and vesicles emerged,together with chromatin condensation and margination.These results suggested that the digestive gland basophilic cells maybe the special target cell of this virus,the infection could cause lesion in digestive gland and digestive organs such as stomach,intestines.Atrophy due to long-term malnutrition and systemic infection would finally led to death.
Pathological analysis was done on juvenile hybrid abalone(Haliotis discus discus Haliotis discus hannai) artificially infected by a spherical virus isolated from the diseased ones in Fuzhou.Examination of artificially infected juvenile hybrid abalone by optical and electron microscopy showed that the virion mainly developed in nucleus of basophilic cells in digestive gland,and led to nuclear heteromorphosis,chromatin condensation,endoplasmic reticulum expansion,and mitochondria cristae breakage in digestive gland cell and basophilic cell.Meanwhile,broken digestive gland lobule basal lamina muscle fibers,autolysis and pyknosis of microvillus and cilium columnar cell in digestive gland intestinal tract were observed.In foot muscle cells,muscle fibers were broken and vesicles emerged,together with chromatin condensation and margination.These results suggested that the digestive gland basophilic cells maybe the special target cell of this virus,the infection could cause lesion in digestive gland and digestive organs such as stomach,intestines.Atrophy due to long-term malnutrition and systemic infection would finally led to death.
2013, 28(5): 438-442.
doi: 10.19303/j.issn.1008-0384.2013.05.006
Abstract:
Transduction of the gall midge resistant gene,Gm6,for rice using the new germplasm resource of Sanming dominant genic male sterile(SMDGMS)rice was studied.Kanwenqingzhan as the donor and SMDGMS rice as the vector,Gm6 was introduced into the rice three-line maintainer line,Yuanfeng B,by successive backcrosses.The result indicated that the pure lines of BSC9246 with Gm6 gene similar to Yuanfeng B in fingerprint and agronomic traits had been selected from the fertile plants of BC5F1.By using Yuanfeng A as the cytoplasmic donor,the three male sterile lines,BSC9247,were bred through successive backcrosses with the heterosis basically identical to Yuanfeng A.This appeared to be an efficient facile method that could not only eliminate the need of the removal of male plants,but also ensure the purity of each generation of the hybrid seeds.
Transduction of the gall midge resistant gene,Gm6,for rice using the new germplasm resource of Sanming dominant genic male sterile(SMDGMS)rice was studied.Kanwenqingzhan as the donor and SMDGMS rice as the vector,Gm6 was introduced into the rice three-line maintainer line,Yuanfeng B,by successive backcrosses.The result indicated that the pure lines of BSC9246 with Gm6 gene similar to Yuanfeng B in fingerprint and agronomic traits had been selected from the fertile plants of BC5F1.By using Yuanfeng A as the cytoplasmic donor,the three male sterile lines,BSC9247,were bred through successive backcrosses with the heterosis basically identical to Yuanfeng A.This appeared to be an efficient facile method that could not only eliminate the need of the removal of male plants,but also ensure the purity of each generation of the hybrid seeds.
2013, 28(5): 443-447.
doi: 10.19303/j.issn.1008-0384.2013.05.007
Abstract:
Effect of N fertilization(i.e.,0,120,180and 240kg·hm-2)and the transplanting density(i.e.,14.1 ×104,18.4×104 and 21.5×104 holes.km-2)on the yield and yield components for super hybrid rice,Yi You 673,were studied in the field experiments conducted in 2012.N fertilizer was found to affect more significantly on the panicle number,grain number per panicle and seed-setting rate than the 1000-grain weight.The effect of transplanting density on the panicle number was higher than those on the grain number per panicle,seed-setting rate and 1000-grain weight.When 180kg·hm-2 N and a transplanting density about 21.5×104 holes.km-2 in the medium-fertility field were applied for the cultivation of Yi You 673m,its yield could be maximized.
Effect of N fertilization(i.e.,0,120,180and 240kg·hm-2)and the transplanting density(i.e.,14.1 ×104,18.4×104 and 21.5×104 holes.km-2)on the yield and yield components for super hybrid rice,Yi You 673,were studied in the field experiments conducted in 2012.N fertilizer was found to affect more significantly on the panicle number,grain number per panicle and seed-setting rate than the 1000-grain weight.The effect of transplanting density on the panicle number was higher than those on the grain number per panicle,seed-setting rate and 1000-grain weight.When 180kg·hm-2 N and a transplanting density about 21.5×104 holes.km-2 in the medium-fertility field were applied for the cultivation of Yi You 673m,its yield could be maximized.
2013, 28(5): 448-451.
doi: 10.19303/j.issn.1008-0384.2013.05.008
Abstract:
Quanshu No.10 was a sweet potato bred from the female parent,Quanshu 267,by open-pollination.The variety was tested and certified by the Crop Variety Approval Committee of Fujian in 2010,as well as the National Crop Variety Identification Committee in 2013.It was chosen as a recommended variety in the Southern Regional Potential Yield Test in 2012.Its annual average fresh root yield was between 37.87and 43.40t·hm-2,which was 9.11%-27.62% higher than that of the reference variety,Jinshan 57 or Guangshu 87.Its annual average dry root yield was 8.91-11.13t·hm-2,which was 1.93%-10.03% higher than those of the reference varieties.The dry matter content of its fresh roots was about 24.9%,which was 1.5% lower than that of Jinshan 57,and 4.5% lower than that of Guangshu 87.The contents of β-carotene and vitamin C were 10.92and 17.45mg·hg-1,respectively,which were 7.6and 0.52mg·hg-1,respectively,higher than those of Jinshan 57.This newly bred variety also exhibited a high resistance to fusarium wilt,bacterial wilt I and bacterial wiltⅡ.
Quanshu No.10 was a sweet potato bred from the female parent,Quanshu 267,by open-pollination.The variety was tested and certified by the Crop Variety Approval Committee of Fujian in 2010,as well as the National Crop Variety Identification Committee in 2013.It was chosen as a recommended variety in the Southern Regional Potential Yield Test in 2012.Its annual average fresh root yield was between 37.87and 43.40t·hm-2,which was 9.11%-27.62% higher than that of the reference variety,Jinshan 57 or Guangshu 87.Its annual average dry root yield was 8.91-11.13t·hm-2,which was 1.93%-10.03% higher than those of the reference varieties.The dry matter content of its fresh roots was about 24.9%,which was 1.5% lower than that of Jinshan 57,and 4.5% lower than that of Guangshu 87.The contents of β-carotene and vitamin C were 10.92and 17.45mg·hg-1,respectively,which were 7.6and 0.52mg·hg-1,respectively,higher than those of Jinshan 57.This newly bred variety also exhibited a high resistance to fusarium wilt,bacterial wilt I and bacterial wiltⅡ.
2013, 28(5): 452-456.
doi: 10.19303/j.issn.1008-0384.2013.05.009
Abstract:
Four humidity levels were applied to air-curing of burley tobaccoto for comparing the effect of curing environment on organic acid content in burley tobacco.The results showed that the highest content of total 20 organic acids was in the lowest humidity level,while with exception of citric acid,the highest content of rest organic acids was found in the middle level of humidity.The content of semi-volatile organic acids and aliphatic acids were decreased following the humidity level increased from low,middle and high to control as the highest humidity applied in air-curing.Some volatile organic acid were undetectable concomitant with the humidity increasing.The content of nonvolatile organic acids was detected the highest in middle humidity of air-curing but the content of citric acid was the lowest.In addition,the highest of malic acid content was found in low humidity curing.
Four humidity levels were applied to air-curing of burley tobaccoto for comparing the effect of curing environment on organic acid content in burley tobacco.The results showed that the highest content of total 20 organic acids was in the lowest humidity level,while with exception of citric acid,the highest content of rest organic acids was found in the middle level of humidity.The content of semi-volatile organic acids and aliphatic acids were decreased following the humidity level increased from low,middle and high to control as the highest humidity applied in air-curing.Some volatile organic acid were undetectable concomitant with the humidity increasing.The content of nonvolatile organic acids was detected the highest in middle humidity of air-curing but the content of citric acid was the lowest.In addition,the highest of malic acid content was found in low humidity curing.
2013, 28(5): 457-462.
doi: 10.19303/j.issn.1008-0384.2013.05.010
Abstract:
In our experiment,15% and 25% polyethylene glycol(PEG 6000)were used to simulate the natural drought conditions.Under the artificial stress,the responses of 60 different varieties of jute(Corchorus L)in seed germination stage were studied.The results showed that the drought-resistance coefficients of the root length,shoot length and germination drought tolerance were reduced significantly as PEG concentration increased.By a cluster analysis,the jutes were,then,categorized into A,B,C,D and E groups each consisting of 18,8,7,3 and 24 varieties,respectively.The jutes in the C and D groups were highly drought tolerant,while those in the E group poorly.Those in the A and the B groups were markedly different in their resistances to drought.In all,16.7% of the varieties tested showed a strong,40% medium and 43.3% weak drought resistance.It appeared that the PEG stress test could be used to evaluate the drought tolerance potential of jute in seed germination stage.
In our experiment,15% and 25% polyethylene glycol(PEG 6000)were used to simulate the natural drought conditions.Under the artificial stress,the responses of 60 different varieties of jute(Corchorus L)in seed germination stage were studied.The results showed that the drought-resistance coefficients of the root length,shoot length and germination drought tolerance were reduced significantly as PEG concentration increased.By a cluster analysis,the jutes were,then,categorized into A,B,C,D and E groups each consisting of 18,8,7,3 and 24 varieties,respectively.The jutes in the C and D groups were highly drought tolerant,while those in the E group poorly.Those in the A and the B groups were markedly different in their resistances to drought.In all,16.7% of the varieties tested showed a strong,40% medium and 43.3% weak drought resistance.It appeared that the PEG stress test could be used to evaluate the drought tolerance potential of jute in seed germination stage.
2013, 28(5): 463-467.
doi: 10.19303/j.issn.1008-0384.2013.05.011
Abstract:
From a cDNA fragment of the suppression subtractive hybridization of Narcissus tazetta var.chinensis induced by paclobutrazol,the gene of the light-harvesting chlorophyll a/b-binding protein,Ntcab1,was isolated and cloned by RACE and RT-PCR.The full length of Ntcab1cDNA was determined to be 1 012bp with a 798bp ORF,which encoded a protein of 265 amino acids with a predicted molecular weight of 28.155kD and a theoretical isoelectric point of 5.48.The phylogenetic analysis showed that Ntcab1could be clustered together with LHCb1.1 from Arabidopsis thaliana,and had a close relationship with those in Ophiopogon japonicus(ACD64978.1)and Lycoris radiata(ADC35579.1).The semi-quantitative RT-PCR revealed that the expression of Ntcab1was higher during sprouting and early flowering after it was sprayed with paclobutrazol than water.
From a cDNA fragment of the suppression subtractive hybridization of Narcissus tazetta var.chinensis induced by paclobutrazol,the gene of the light-harvesting chlorophyll a/b-binding protein,Ntcab1,was isolated and cloned by RACE and RT-PCR.The full length of Ntcab1cDNA was determined to be 1 012bp with a 798bp ORF,which encoded a protein of 265 amino acids with a predicted molecular weight of 28.155kD and a theoretical isoelectric point of 5.48.The phylogenetic analysis showed that Ntcab1could be clustered together with LHCb1.1 from Arabidopsis thaliana,and had a close relationship with those in Ophiopogon japonicus(ACD64978.1)and Lycoris radiata(ADC35579.1).The semi-quantitative RT-PCR revealed that the expression of Ntcab1was higher during sprouting and early flowering after it was sprayed with paclobutrazol than water.
2013, 28(5): 468-471.
doi: 10.19303/j.issn.1008-0384.2013.05.012
Abstract:
Through optimization of the concentrations of Taq DNA polymerase,dNTP,primers and DNA template,SRAP-PCR of Abelmoschus esculentus Linn.was established.The results indicated that,in a 20μL reaction mixture,the optimal concentration of genomic DNA template was 75ng;that of the primers,0.4mmol·L-1;that of dNTP,0.75mmol·L-1;that of Taq DNA,polymerase,1.5U;and that of MgCl2,2.0mmol·L-1.
Through optimization of the concentrations of Taq DNA polymerase,dNTP,primers and DNA template,SRAP-PCR of Abelmoschus esculentus Linn.was established.The results indicated that,in a 20μL reaction mixture,the optimal concentration of genomic DNA template was 75ng;that of the primers,0.4mmol·L-1;that of dNTP,0.75mmol·L-1;that of Taq DNA,polymerase,1.5U;and that of MgCl2,2.0mmol·L-1.
2013, 28(5): 472-477.
doi: 10.19303/j.issn.1008-0384.2013.05.013
Abstract:
The compositions and contents of sugars and amino acids in fruits of Chinese olive from 5different cultivars(strains)were analyzed.The results showed that the contents of total soluble sugar,sucrose and reducing sugar in the fruits of Chinese olive were between 2.43% ~4.47%,1.08% ~2.90% and 1.15% ~1.48%,respectively.All fruits of Chinese olive from 5cultivars(strains)contained 17 kinds of amino acids,including seven human body essential amino acids such as phenylalanine,threonine,lysine and so on(tryptophan not detected),two children essential amino acids(histidine and arginine),two semi-essential amino acids(cystine and tyrosine)and 6 kinds of non-essential amino acids.The total content of amino acids in the fruits of Chinese olive from 5different cultivars(strains)ranged from 688.26 to 1 109.52mg·hg-1.The ratios of essential amino acids,taste amino acids and medicinal amino acids to total amino acids were between 24.13%~25.80%,90.41%~91.47% and 67.98%~ 72.97%,respectively.There was correlation between the contents of soluble sugars and amino acids in fruits of Chinese olive.The content of total soluble sugar was highly significantly positive correlated with the content of sucrose,and significant positive correlated with the contents of sweet amino acids such as serine,alanine and histidine.The content of reducing sugar was significant positive correlated with the content of aspartic acid.
The compositions and contents of sugars and amino acids in fruits of Chinese olive from 5different cultivars(strains)were analyzed.The results showed that the contents of total soluble sugar,sucrose and reducing sugar in the fruits of Chinese olive were between 2.43% ~4.47%,1.08% ~2.90% and 1.15% ~1.48%,respectively.All fruits of Chinese olive from 5cultivars(strains)contained 17 kinds of amino acids,including seven human body essential amino acids such as phenylalanine,threonine,lysine and so on(tryptophan not detected),two children essential amino acids(histidine and arginine),two semi-essential amino acids(cystine and tyrosine)and 6 kinds of non-essential amino acids.The total content of amino acids in the fruits of Chinese olive from 5different cultivars(strains)ranged from 688.26 to 1 109.52mg·hg-1.The ratios of essential amino acids,taste amino acids and medicinal amino acids to total amino acids were between 24.13%~25.80%,90.41%~91.47% and 67.98%~ 72.97%,respectively.There was correlation between the contents of soluble sugars and amino acids in fruits of Chinese olive.The content of total soluble sugar was highly significantly positive correlated with the content of sucrose,and significant positive correlated with the contents of sweet amino acids such as serine,alanine and histidine.The content of reducing sugar was significant positive correlated with the content of aspartic acid.
2013, 28(5): 478-482.
doi: 10.19303/j.issn.1008-0384.2013.05.014
Abstract:
With Huangdan as the control species(CK),the national regional test of a new tea variety-Dangui was conducted for 6 years in Guangdong,Guangxi and Hu′nan provinces.The characteristics of Dangui in the regional test were detected including the rate of survival,growth potential,phenological phase,yield of fresh leaves,quality of made tea and its environmental resistances.The results showed that Dangui grew well,and had the high rate of survival,early phenological phase and strong environmental resistance,as well as high yield of fresh leaves and good quality of made tea.It could be an early-maturing,excellent-quality and high-yielding tea variety,which had good adaptability in plantation,popularization and application in Guangdong,Guangxi and Hunan provinces and other Oolong tea regions.
With Huangdan as the control species(CK),the national regional test of a new tea variety-Dangui was conducted for 6 years in Guangdong,Guangxi and Hu′nan provinces.The characteristics of Dangui in the regional test were detected including the rate of survival,growth potential,phenological phase,yield of fresh leaves,quality of made tea and its environmental resistances.The results showed that Dangui grew well,and had the high rate of survival,early phenological phase and strong environmental resistance,as well as high yield of fresh leaves and good quality of made tea.It could be an early-maturing,excellent-quality and high-yielding tea variety,which had good adaptability in plantation,popularization and application in Guangdong,Guangxi and Hunan provinces and other Oolong tea regions.
2013, 28(5): 483-485.
doi: 10.19303/j.issn.1008-0384.2013.05.015
Abstract:
Fumei 1was a new strawberry variety derived from crossing Toyonoka and Fualandi.It had a strong growth potential and high resistance to the powdery mildew.Its conical fruits were red in color,delicate in texture,sweet and juicy in taste,and fragrant in aroma.The seeds were slightly submerged under the surface of the flesh.The fruit′s soluble solid content was 8.5%;the total soluble sugars,6.6%;the titratable acid,0.75%;and,vitamin C,564.00 mg·kg-1.The single fruit weight was 22g on average,and the fruit productivity was approximately 40 500kg·hm-2.
Fumei 1was a new strawberry variety derived from crossing Toyonoka and Fualandi.It had a strong growth potential and high resistance to the powdery mildew.Its conical fruits were red in color,delicate in texture,sweet and juicy in taste,and fragrant in aroma.The seeds were slightly submerged under the surface of the flesh.The fruit′s soluble solid content was 8.5%;the total soluble sugars,6.6%;the titratable acid,0.75%;and,vitamin C,564.00 mg·kg-1.The single fruit weight was 22g on average,and the fruit productivity was approximately 40 500kg·hm-2.
2013, 28(5): 486-489.
doi: 10.19303/j.issn.1008-0384.2013.05.016
Abstract:
The shoot tip of Spathiphyllum 'Vicki' was used as explant to study its conditions for rapid propagation, including disinfection time, bud proliferation and rooting culture, and so on. The result showed the best disinfection time of 0.1%HgClz was 8 rain. The best shoot induction medium was MS+BA 1.5 mg· L-1 and the induce rate was up to 86%. The best bud proliferation medium was MS+BA2.0+KT0.2+NAA0. 3, and the multiplication coefficient was 3.6. The best rooting medium was 2/3MS+IBA1.5+AC 0.15% +sucrose 3%, and the rooting rate was 95 ~. The peat soil. and pearlite matrix had a ratio of 10 : 1, which was suitable for transplanting.
The shoot tip of Spathiphyllum 'Vicki' was used as explant to study its conditions for rapid propagation, including disinfection time, bud proliferation and rooting culture, and so on. The result showed the best disinfection time of 0.1%HgClz was 8 rain. The best shoot induction medium was MS+BA 1.5 mg· L-1 and the induce rate was up to 86%. The best bud proliferation medium was MS+BA2.0+KT0.2+NAA0. 3, and the multiplication coefficient was 3.6. The best rooting medium was 2/3MS+IBA1.5+AC 0.15% +sucrose 3%, and the rooting rate was 95 ~. The peat soil. and pearlite matrix had a ratio of 10 : 1, which was suitable for transplanting.
2013, 28(5): 490-493.
doi: 10.19303/j.issn.1008-0384.2013.05.017
Abstract:
The histological observation for the sex-pheromone gland of female Conopomorpha sinensis was conducted with scanning electron(SEM)and transmission electron microscopy(TEM).From images,it was clearly observed that the gland was a ventral fold cystiform situated in the dorsal segmental membrane between the eighth and ninth segments,its surface distributed with many conical objects.There was distinct intercellular conjugation with a tubular channel connected to the endepidermis.Microvilli were distributed on the basal fold of membrane.In the cytoplasm,there were obviously with smooth endoplasmic reticulum,mitochondria,ribosome,and glycogen granule.
The histological observation for the sex-pheromone gland of female Conopomorpha sinensis was conducted with scanning electron(SEM)and transmission electron microscopy(TEM).From images,it was clearly observed that the gland was a ventral fold cystiform situated in the dorsal segmental membrane between the eighth and ninth segments,its surface distributed with many conical objects.There was distinct intercellular conjugation with a tubular channel connected to the endepidermis.Microvilli were distributed on the basal fold of membrane.In the cytoplasm,there were obviously with smooth endoplasmic reticulum,mitochondria,ribosome,and glycogen granule.
2013, 28(5): 494-498.
doi: 10.19303/j.issn.1008-0384.2013.05.018
Abstract:
To investigate the final residues and dynamic degradation of deltamethrin sprayed on Fuyun 6,Jinguanyin,Tieguanyin and Shuixian(Camellia sinensis),a security analysis of residual deltamethrin in different tea plantation(Fu′an,Anxi and Wuyishan)was carried out after 500-3 000 times of 2.5% deltamethrin was air-dried or 1,3,5,7,10,15 days after spraying.The results showed that the reduce residues has a linear relation with diluted times of the pesticides.On the one hand,the residual amounts treated with the same concentrations were higher in spring tea than those of summer tea or autumn tea,and more residual contents would be found in green tea varieties(Fuyun 6) than those of oolong tea varieties(Jinguanyin).The half-life of the degradation was prolonged with the increased concentrations but with no significant differences.On the other hand,oolong teas had a long half-life of the degradation than those of green teas at the same concentrations,which respectively reached a significant level in spring.And there were significant difference of half-life among oolong tea varieties in every season sprayed with the same concentration of pesticides in each region.The usages of 500-3 000 times of 2.5% deltamethrin in the tea garden were relative safety concentrations.The status quo requirement of 5 days as the safety interval was appropriate according to the proposal of GB/T 8321.1-2000,Guideline for safety application of pesticides(Ⅰ),but 7days was recommended for non-polluted tea garden and export base to the European Union member countries.
To investigate the final residues and dynamic degradation of deltamethrin sprayed on Fuyun 6,Jinguanyin,Tieguanyin and Shuixian(Camellia sinensis),a security analysis of residual deltamethrin in different tea plantation(Fu′an,Anxi and Wuyishan)was carried out after 500-3 000 times of 2.5% deltamethrin was air-dried or 1,3,5,7,10,15 days after spraying.The results showed that the reduce residues has a linear relation with diluted times of the pesticides.On the one hand,the residual amounts treated with the same concentrations were higher in spring tea than those of summer tea or autumn tea,and more residual contents would be found in green tea varieties(Fuyun 6) than those of oolong tea varieties(Jinguanyin).The half-life of the degradation was prolonged with the increased concentrations but with no significant differences.On the other hand,oolong teas had a long half-life of the degradation than those of green teas at the same concentrations,which respectively reached a significant level in spring.And there were significant difference of half-life among oolong tea varieties in every season sprayed with the same concentration of pesticides in each region.The usages of 500-3 000 times of 2.5% deltamethrin in the tea garden were relative safety concentrations.The status quo requirement of 5 days as the safety interval was appropriate according to the proposal of GB/T 8321.1-2000,Guideline for safety application of pesticides(Ⅰ),but 7days was recommended for non-polluted tea garden and export base to the European Union member countries.
2013, 28(5): 499-503.
doi: 10.19303/j.issn.1008-0384.2013.05.019
Abstract:
To determine the role and effect of the financial aid for the agricultural modernization of Jiangsu,relevant achievements and existing problems were examined.The study indicated that the aid had strengthened and enhanced the agricultural industrialization,large-scale production,science and technology,labor force quality,infrastructure,mechanization,information technology and service system in Jiangsu.Nonetheless,further improvements and refinements of the operations on capital investment,structure,efficiency,mechanism design and management system could be implemented.This evaluation not only presents the recommendations for future development and policy formulation for the province,but also summarizes the experience that could benefit the agricultural modernization of the nation.
To determine the role and effect of the financial aid for the agricultural modernization of Jiangsu,relevant achievements and existing problems were examined.The study indicated that the aid had strengthened and enhanced the agricultural industrialization,large-scale production,science and technology,labor force quality,infrastructure,mechanization,information technology and service system in Jiangsu.Nonetheless,further improvements and refinements of the operations on capital investment,structure,efficiency,mechanism design and management system could be implemented.This evaluation not only presents the recommendations for future development and policy formulation for the province,but also summarizes the experience that could benefit the agricultural modernization of the nation.
2013, 28(5): 504-508.
doi: 10.19303/j.issn.1008-0384.2013.05.020
Abstract:
According to the good inheritance and development situation of panyong congou industry,the eight major science and technology innovation of panyong congou in the production were analyzed.① Tea resources and innovation and application of the high-aroma pattern varieties;②Integration and innovation of the low-carbon,efficient and ecological cultivation technology;③Innovation of processing technology on the flower-fruit aroma black tea;④The development of standard and innovation of processing machinery;⑤Originality and innovation of packaging and culture;⑥Innovation of selling idea and network platform;⑦Skill innovation of drinking and brewing process;⑧Model innovation of brand construction.Then the creativeness ideas of trade name,packaging,culture,journey on panyong congou were investigate.Finally the industry development countermeasure were put forwarded,including stabilizing area of tea garden,taking advantage of variety,development characteristic product,supporting system authentication,implementation of GAP management,establishing tracing back system,guiding transformation of production mode,developing low-carbon,high profit and product differentiation road,attempting tea-manor management.The purpose was to promote transformation and upgrading of panyong congou black tea industry.
According to the good inheritance and development situation of panyong congou industry,the eight major science and technology innovation of panyong congou in the production were analyzed.① Tea resources and innovation and application of the high-aroma pattern varieties;②Integration and innovation of the low-carbon,efficient and ecological cultivation technology;③Innovation of processing technology on the flower-fruit aroma black tea;④The development of standard and innovation of processing machinery;⑤Originality and innovation of packaging and culture;⑥Innovation of selling idea and network platform;⑦Skill innovation of drinking and brewing process;⑧Model innovation of brand construction.Then the creativeness ideas of trade name,packaging,culture,journey on panyong congou were investigate.Finally the industry development countermeasure were put forwarded,including stabilizing area of tea garden,taking advantage of variety,development characteristic product,supporting system authentication,implementation of GAP management,establishing tracing back system,guiding transformation of production mode,developing low-carbon,high profit and product differentiation road,attempting tea-manor management.The purpose was to promote transformation and upgrading of panyong congou black tea industry.
2013, 28(5): 509-512.
doi: 10.19303/j.issn.1008-0384.2013.05.021
Abstract:
Southern corn rust,caused by Puccinia polysora Unedrw.,is an important maize disease in corn producing areas.The disease results in reducing the yield and grain quality by damaging the corn leaves,stalks,bract and tassel organization.This paper aimed to review the research progresses for southern corn rust worldwide on occurrence and damage analysis,symptom observation and pathogenic characteristics,genetic analysis of the resistance genus,comprehansive prevention,as well as to discuss further research in resolving of the disease.
Southern corn rust,caused by Puccinia polysora Unedrw.,is an important maize disease in corn producing areas.The disease results in reducing the yield and grain quality by damaging the corn leaves,stalks,bract and tassel organization.This paper aimed to review the research progresses for southern corn rust worldwide on occurrence and damage analysis,symptom observation and pathogenic characteristics,genetic analysis of the resistance genus,comprehansive prevention,as well as to discuss further research in resolving of the disease.
2013, 28(5): 513-515.
doi: 10.19303/j.issn.1008-0384.2013.05.022
Abstract:
An L9(33)orthogonal experiment was used to optimize the conditions for the sticking of Syzygium samarangense.The results showed that the highest root survival rate could be reached if the growth regulator,IBA,was applied at 400 mg·L-1for 10 min;the greatest number of roots was found when the application was 600 mg·L-1for 10min;and,the longest average root length was achieved when the application was 400mg·L-1 for 30min.It was concluded that the most desirable overall effect,i.e.,a root survival rate of 90%,16.6 roots per plant,and 7.43cm in root length on average,could be obtained with a pre-soaking of S.samarangense cutting in a 400 mg·L-1IBA solution for 10min prior to the sticking.
An L9(33)orthogonal experiment was used to optimize the conditions for the sticking of Syzygium samarangense.The results showed that the highest root survival rate could be reached if the growth regulator,IBA,was applied at 400 mg·L-1for 10 min;the greatest number of roots was found when the application was 600 mg·L-1for 10min;and,the longest average root length was achieved when the application was 400mg·L-1 for 30min.It was concluded that the most desirable overall effect,i.e.,a root survival rate of 90%,16.6 roots per plant,and 7.43cm in root length on average,could be obtained with a pre-soaking of S.samarangense cutting in a 400 mg·L-1IBA solution for 10min prior to the sticking.
