外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响

胡牮; 林雅楠; 吴楠; 蒋诗睿; 宋杨; 张建新; 郑玲霞; 陈祺月; 刘鹏

doi:10.19303/j.issn.1008-0384.2024.04.011

外源褪黑素对低温逆境下三角梅生理特性和细胞壁组分的影响

doi: 10.19303/j.issn.1008-0384.2024.04.011

胡牮^1,,
林雅楠²,
吴楠²,
蒋诗睿²,
宋杨²,
张建新¹,
郑玲霞²,
陈祺月²,
刘鹏^2, ,

1.
丽水职业技术学院，浙江丽水　323000
2.
浙江师范大学植物学实验室，浙江金华　321004

基金项目: 国家自然科学基金项目（32001224、30540056）；浙江省公益研究计划项目（LGN19C140009）；浙江大花园建设研究院2021年专项课题（DHYB1001）

详细信息

作者简介:
胡牮（1985 —），男，硕士，副教授，主要从事植物逆境生理、园林植物及其应用研究，E-mail：hudainiu@163.com

通讯作者:
刘鹏（1965−），男，博士，教授，主要从事植物逆境生理、环境污染与保护及植物生理生态研究，E-mail：sky79@zjnu.cn

中图分类号: S685.99
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出版历程
- 收稿日期: 2024-01-04
- 修回日期: 2024-03-07
- 网络出版日期: 2024-05-08
- 刊出日期: 2024-04-28

Effects of Melatonin Application on Physiology and Cell Wall of Bougainvillea Plant under Cold Stress

1.
Lishui Vocational and Technical College, Lishui, Zhejiang　323000, China
2.
Key Laboratory of Botany, Zhejiang Normal University, Jinhua, Zhejiang　321004, China

摘要

摘要: 目的研究褪黑素对低温逆境下三角梅生理生化及细胞壁组分的调控作用，为缓解低温对三角梅生长造成的不利影响提供理论依据。方法以不同耐冷性的3个三角梅品种安格斯、口红、变色龙为供试材料，在4 ℃低温下对三角梅植株叶面施加不同浓度（0、50、100、150 µmol·L^–1）的外源褪黑素，测定各处理组生长0、7、14、21 d的叶面积、叶绿素含量、叶绿素荧光参数[PSⅡ最大光化学量子产量（F_v/F_m）、表观电子传递速率（ETR）、光化学猝灭系数（qP）、非光化学猝灭系数（qN）]、抗氧化酶[超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）]活性、膜脂过氧化产物[超氧阴离子（O₂⁻）产生速率、过氧化氢（H₂O₂）含量]、渗透调节物质[可溶性糖（SS）、可溶性蛋白（SP）]含量、细胞壁组分[螯合型果胶（CSP）、碱溶性果胶（SSP）、半纤维素（HC）和纤维素（CE）]含量。结果低温逆境下三角梅的正常生长发育受阻，叶绿素荧光参数失衡，膜脂过氧化加剧，细胞壁组分受到影响。而施加100 µmol·L^–1的褪黑素能够有效地提升三角梅的生理活性，改善植株萎蔫程度，F_v/F_m与ETR降幅减小；抗氧化酶活性显著增加，强耐冷型品种安格斯较于0 d的SOD、POD、CAT最大可增加96.45%、104.35%、73.11%；H₂O₂含量、O₂⁻生成速率对比同周期MT₀最多下降21.07%、26.85%；细胞壁组分中CSP、SSP上升幅度增加，较于0 d最多可上涨22.55%、43.08%。结论明确了外源褪黑素在生理特性方面对不同品种三角梅冷害的调控作用，外源施加适宜浓度的褪黑素可以有效缓解低温对三角梅生长的抑制，减轻光系统损伤，增强抗氧化酶活性，减缓膜脂氧化，并调节细胞壁各组分含量以维持其结构整体稳定，从而降低低温伤害，为探索褪黑素对冷害下植物的调控作用提供新思路。
- 褪黑素 /
- 三角梅 /
- 低温逆境 /
- 生理响应 /
- 细胞壁组分
Abstract: Objective Effects of melatonin application on plant physiology and biochemistry as well as cell wall components of Bougainvillea plants under low temperature were studied. Method Bougainvillea leaves were sprayed with melatonin solutions in the concentrations of 0, 50, 100, and 150 µmol·L^–1 at 4 ℃. Leaf area, chlorophyll content, chlorophyll fluorescence parameters at the optimal/maximal quantum yield of PSⅡ (F_v/F_m), apparent electron transfer rate (ETR), photochemical quenching coefficient (qP), non-photochemical quenching coefficient (qN)], activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), membrane lipid peroxidation products at the production rate of superoxide anion (O₂⁻), hydrogen peroxide (H₂O₂) content, osmotic adjustment soluble sugar (SS) and soluble protein (SP) as well as cell wall components including chelate pectin (CSP), alkali-soluble pectin (SSP), hemicellulose (HC), and cellulose (CE) of the plants were monitored weekly for 3 consecutive weeks from the beginning of treatments (MT₀). Result At 4 ℃, the growth and development of Bougainvillea plants was hindered showing unbalanced chlorophyll fluorescence parameters, intensified membrane lipid peroxidation, and altered cell wall composition. The spray of 100 µmol·L⁻¹ melatonin significantly enhanced the physiological activity, reduced wilting, F_v/F_m and ETR, increased activities of SOD, POD, and CAT (e.g., by 96.45%, 104.35%, and 73.11%, respectively, in Elizabeth Angus), decreased H₂O₂ content by 21.07% and O₂⁻ production rate by 26.85%, and raised CSP by 22.55% and SSP by 43.08% in the cell wall over those at MT₀. Conclusion Melatonin regulated the physiology of Bougainvillea plants and effectively mitigated the growth inhibition by cold stress. The spray reduced the damage to the photosystem, enhance the antioxidant enzyme activity, retard the membrane lipid oxidation, and stabilize the cell wall of the plant suggesting a potential treatment for preventing serious harms by temperature decline in winter.
- Melatonin /
- Bougainvillea /
- cold stress /
- physiological response /
- cell wall composition

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图 1 MT处理对低温下三角梅表观性状的影响

Figure 1. Effects of MT at 4 ℃ on physiology of Bougainvillea plants

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图 2 MT处理对低温下三角梅叶绿素含量的影响

不同小写字母表示同一品种不同处理间差异显著（P＜0.05），下同。

Figure 2. Effect of MT at 4 ℃ on chlorophyll content of Bougainvillea plants

Data with different lowercase indicate significant differences on same Bougainvillea variety at P＜0.05. Same for below.

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图 3 MT处理对低温下三角梅叶绿素荧光参数的影响

Figure 3. Effect of MT at 4 ℃ on chlorophyll fluorescence parameters of Bougainvillea plants

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图 4 MT处理对低温下三角梅过氧化氢含量和超氧阴离子产生速率的影响

Figure 4. Effect of MT at 4 ℃ on hydrogen peroxide content and superoxide anion production rate of Bougainvillea plants

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图 5 MT处理对低温下三角梅抗氧化酶系统的影响

Figure 5. Effect of MT at 4 ℃ on antioxidant oxidases of Bougainvillea plants

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图 6 MT处理对低温下三角梅可溶性糖和可溶性蛋白含量的影响

Figure 6. Effect of MT at 4 ℃ on contents of soluble sugar and soluble protein in Bougainvillea plants

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图 7 MT处理对低温下三角梅细胞壁组分的影响

Figure 7. Effect of MT at 4 ℃ on cell wall components of Bougainvillea plants

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