多效唑和乙烯利对西克刺桐花芽分化及成花基因表达的影响

刘贝宁; 陈发兴

doi:10.19303/j.issn.1008-0384.2024.04.007

多效唑和乙烯利对西克刺桐花芽分化及成花基因表达的影响

doi: 10.19303/j.issn.1008-0384.2024.04.007

刘贝宁^1,,
陈发兴^2, ,

1.
福建农林大学戴尔豪西大学联合学院（国际学院），福建　福州　350002
2.
福建农林大学园艺学院，福建　福州　350002

基金项目: 福建省科技计划项目（2022N5016）

详细信息

作者简介:
刘贝宁（2003 —），女，主要从事园林植物研究，E-mail：2075431492@qq.com

通讯作者:
陈发兴（1967 —），男，博士，教授，主要从事园艺植物领域研究，E-mail：fxchen@fafu.edu.cn

中图分类号: S685
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- 被引次数: 0
出版历程
- 收稿日期: 2024-03-05
- 修回日期: 2024-04-04
- 网络出版日期: 2024-06-26
- 刊出日期: 2024-04-28

Effects of Paclobutrazol and Ethephon on the Differentiation of Flower Buds and Expression of Flowering Genes in Erythrina sykesii

LIU Beining^1
,,
CHEN Faxing^{2
, ,}

1.
FAFU-Dal Joint College（International College）, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

摘要

摘要: 目的探讨多效唑（paclobutrazol, PAC）和乙烯利（ethephon, ETH）对西克刺桐（Erythrina sykesii）碳氮代谢、内源激素及成花相关基因表达的影响，为刺桐属植物花期调控提供理论依据。方法以8 年生的西克刺桐为材料，在花芽生理分化期进行多效唑（PAC）600 mg·L⁻¹、乙烯利（ETH）50 mg·L⁻¹以及二者共同喷施处理，以清水为对照（CK），每个处理均喷施3次。检测顶芽不同花芽生理分化期的碳氮代谢物质含量、内源激素水平和成花相关基因表达量，并调查统计盛花期西克刺桐花序大小、数量和枝条成花率。结果西克刺桐叶面喷施多效唑和乙烯利后，随着花芽生理分化进程推进，顶芽的可溶性糖和全碳含量逐渐上升，而可溶性蛋白和全氮含量逐渐下降，导致碳氮比（C/N）升高；其中，PAC+ETH处理与PAC和ETH处理均存在显著差异，在生理分化末期PAC+ETH处理的C/N比值达到最大值。顶芽的内源激素含量也随着生理分化进程而变化，玉米素核苷（zeatin riboside, ZR）和脱落酸（abscisic acid, ABA）含量逐渐上升，而赤霉素（gibberellic acid, GA₃）和吲哚乙酸（indole-3-acetic acid, IAA）含量逐渐下降；引起ABA/IAA、ABA/GA₃、ZR/IAA、ZR/GA₃比值逐渐升高；PAC+ETH处理的激素比值与PAC和ETH处理间均存在显著差异，在生理分化末期PAC+ETH处理的ABA/IAA、ABA/GA₃、ZR/IAA、ZR/GA₃比值均达到最大值，分别比对照提高317.49%、185.34%、310.58%、180.62%。成花促进基因FT在生理分化中期开始表达且表达量逐渐上调，SOC1、AP1、SVP和LFY基因在生理分化末期才明显表达；成花抑制基因TFL1在生理分化前期就开始表达且表达量逐渐下调。多效唑和乙烯利处理均能促进西克刺桐花芽分化进程和成花诱导，其中600 mg·L⁻¹ PAC+50 mg·L⁻¹ ETH处理的植株开花期提前12 d，枝条成花率达36.46%，植株总花期达55 d。结论西克刺桐花芽生理分化期喷施多效唑和乙烯利有利于提高碳氮代谢物质含量，调节内源激素水平和成花相关基因表达，有效促进西克刺桐花芽分化。
- 西克刺桐 /
- 花芽诱导 /
- 植物生长调节剂 /
- 成花基因 /
- 内源激素
Abstract: Objective To explore the effects of paclobutrazol (PAC) and ethephon (ETH) on carbon and nitrogen metabolism, endogenous hormone levels, and flower-related gene expression in the flowering plant of Erythrina sykesii, and provide theoretical basis for regulating the flowering period of E. sykesii. Methods Eight-year-old E. sykesii. were treated with three sprays of PAC (600 mg·L⁻¹) and ETH (50 mg·L⁻¹) during the bud physiological differentiation stage, with distilled water as the control (CK). The contents of carbon and nitrogen metabolites, endogenous hormone levels, and flower-related gene expression in the top buds were detected during different bud physiological differentiation periods, and the inflorescence size and number, and branch flowering rate were investigated and statistically analyzed during the peak flowering period. Results After spraying PAC and ETH on the leaves of E. sykesii., the soluble sugar and total carbon (C) content in the top buds gradually increased with the progress of physiological differentiation, while the soluble protein and total nitrogen (N) content gradually decreased, resulting in an increase in the C/N ratio. Among them, there were significant differences between the PAC+ETH treatment and the PAC and ETH treatments, with the PAC+ETH treatment reaching the maximum C/N ratio at the end of physiological differentiation. The endogenous hormone content in the top buds also changed with the physiological differentiation process, with zeatin riboside (ZR) and abscisic acid (ABA) content gradually increasing, while gibberellic acid (GA₃) and indole-3-acetic acid (IAA) content gradually decreasing; resulting in a gradual increase in the ratios of ABA/IAA, ABA/GA₃, ZR/IAA, and ZR/GA₃. There were significant differences between the PAC+ETH treatment and the PAC and ETH treatments, with the PAC+ETH treatment reaching the maximum ABA/IAA, ABA/GA₃, ZR/IAA, and ZR/GA₃ ratios at the end of physiological differentiation, which were 317.49%, 185.34%, 310.58%, and 180.62% higher than the control, respectively. The flowering-promoting gene Flowering Locus T (FT) began to express and the gene amount gradually increased during the middle stage of physiological differentiation, while SUPPRESSOR OF OVEREXPRESSION OF CONSTANS1 (SOC1), APETALA 1 (AP1), SHORT VEGETATIVE PHASE (SVP), and LEAFY (LFY) genes were significantly expressed at the end of physiological differentiation. The flowering-inhibiting gene TERMINAL FLOWER 1 (TFL1) began to express and the gene amount gradually decreased during the early stage of physiological differentiation. Both paclobutrazol and ethephon treatments promoted the flowering bud differentiation process and flowering induction of E. sykesii. The plant treated with PAC+ETH had an advanced flowering period of 12 days, a flowering rate of 36.46%, and a total flowering period of 55 days. Conclusion During the physiological differentiation stage of the flower buds of E. sykesii., spraying paclobutrazol and ethenol is conducive to enhancing the content of carbon and nitrogen metabolites, modulating endogenous hormone levels, stimulating the expression of flowering-related genes, and effectively facilitating floral bud differentiation in E. sykesii.
- Erythrina sykesii /
- Flower bud induction /
- Plant growth regulator /
- Floral genes /
- Endogenous hormones

HTML全文

图 1 多效唑和乙烯利对西克刺桐碳氮代谢的影响

图中不同小写字母表示处理间存在显著性差异 (P＜0.05)。下同。

Figure 1. Effects of paclobutrazol and ethephon on carbon and nitrogen substances in E. sykesii

Different lowercase letters indicate significant difference at 0.05 level among treatments. Same for below.

下载: 全尺寸图片幻灯片

图 2 多效唑和乙烯利对西克刺桐内源激素含量的影响

Figure 2. Effects of paclobutrazol and ethephon on endogenous hormone content in E. sykesii

下载: 全尺寸图片幻灯片

图 3 多效唑和乙烯利对西克刺桐内源激素平衡的影响

Figure 3. Effects of paclobutrazol and ethephon on the ratio of endogenous hormones in E. sykesii

下载: 全尺寸图片幻灯片

图 4 多效唑和乙烯利对西克刺桐成花相关基因表达的影响

Figure 4. Effects of paclobutrazol and ethephon on the expression of flowering genes in E. sykesii

下载: 全尺寸图片幻灯片

表 1 表１西克刺桐成花相关基因定量PCR引物序列

Table 1. Primers designed for flower related genes in E. sykesii

基因 Gene	前引物/后引物 Forward/ Reverse primers	序列（5'-3'） Sequence（5'-3'）
TIP41	TIP41-F	GGGTTGGATGGAAGAAGAAGCAC
TIP41	TIP41-R	CACTTGCTGTGACGGTTTACTTC
TFL1	TFL1-F	GGCAGAGGAGAGGACAGGTA
TFL1	TFL1-R	AAGAGAGTGTCAGTCAGCGG
LFY	LFY-F	AGAGGGAGCATCCGTTTATCGT
LFY	LFY-R	CGTACCTGAATACTTGGTTCGTCAC
AP1	AP1-F	TGAACATGGGTGGCAATTAC
AP1	AP1-R	TGTCAAATGCCATACCAAAG
SVP	SVP-F	GATTGAGCAAGGAAGTTGCC
SVP	SVP-R	TCTTCTCTCCCTTCTTTTCTATTAT
FT	FT-F	AGTCCTAGCAACCCTCACCTCC
FT	FT-R	GTCTTCTTCCTCCGCAGCCACT
SOC1	SOC1-F	TTGTGATGCTGAAGTTGCTCTCAT
SOC1	SOC1-R	ACTCCTGTTATGCCTGCGGTAG

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表 2 多效唑和乙烯对西克刺桐成花的影响

Table 2. Effects of paclobutrazol on the flowering of E. sykesii

处理 Treatment	花序长 Inflorescence length/cm	花序宽 Inflorescence width/cm	花序小花数 Small flowers number	枝条成花率 Flower formation rate/%	始花日期 Early flowering date	较对照提前天数 Earlier than the control/d	总花期 Total flowering time/d
CK	18.3±1.6 a	12.4±1.2 a	66.7±3.9 a	13.02±1.31 c	2023-02-06	0	46
PAC	15.1±4.5 b	12.5±2.4 a	65.1±5.2 a	21.82±1.74 b	2023-02-01	5	54
ETH	18.6±1.4 a	12.7±1.6 a	68.8±6.1 a	26.75±2.91 b	2023-01-30	7	52
PAC+ETH	16.2±0.8 b	12.9±5.8 a	67.3±4.1 a	36.46±4.85 a	2023-01-25	12	55
同列数据后不同小写字母表示不同处理间差异达到显著水平（P＜0.05）。 Different lowercase letters in the same column indicate significant differences among different treatments at 0.05 level.

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