Breeding and Properties of Hybrid Rice Yexiangyou 699
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摘要:
目的 选育优质、抗病的杂交稻品种在生产上推广和应用,满足市场需求,提高种粮效益。 方法 通过水稻优异基因单倍型背景分析,以产量高、抗病性好、恢复力强、配合力好的恢复系福恢699为父本,与香型、优质、抗病的三系不育系野香A杂交配组,杂种F1代经多年多点稻瘟病圃抗性鉴定,多生态区适应性筛选和稻米品质分析等相结合,选育符合育种目标性状的优良杂交稻品种。 结果 2017—2018年参加福建省中稻区域试验,表现群体整齐,株型适中,全生育期两年平均140.4 d,比对照II优3301早熟2.8 d。有效穗数208.5万穗·hm−2,平均株高135.4 cm,结实率86.89%,千粒重26.3 g。产量平均9404.3 kg·hm−2,比对照增产1.50%,不显著;野香优699聚合了来自双亲的7个稻瘟病抗性基因和2个水稻病毒病抗性基因,两年区试田间和室内稻瘟病抗性鉴定综合评价均抗稻瘟病,生产上也表现较强的抗病性。 结论 野香优699(闽审稻20200012)糙米率80.1%,整精米率68.5%,垩白度0.8%,透明度1级,碱消值5.5,胶稠度86 mm,直链淀粉含量14.0%,米质达部颁三等优质食用稻品种品质标准,田间表现产量高、抗病性强,在生产上可大面积推广应用。 Abstract:Objective A disease-resistant, high-quality hybrid rice was bred to meet the market demand and improve the profitability for the farmers. Method Based on the desired gene haplotypes, the high-yield, disease-resistant, strong-restoring and combining Fuhui 699 restorer line was selected as the male parent to cross with the female parents, the 3 sterile Yexiang A lines that are known for their desirable fragrance, high quality, and strong disease-resistance. The resulting F1 hybrid was examined for the target traits at multi-year and multi-point rice blast resistance tests as well as multi-ecological adaptability and rice quality analyses. Result From 2017 to 2018, the choice hybrid, Yexiangyou 699, was submitted to the regional trial on mid-season rice in Fujian. It displayed an evenly distributed population of acceptable plant type and delivered a two-year averaged growth period of 140.4 d, which was 2.8 d shorter than the reference, II You 3301. The effective panicle number of the cultivar averaged 2.085 million panicles·hm−2, the plant height 135.4 cm, the seed setting rate 86.89%, and the 1000-grain weight 26.3 g. The average crop yield was 9 404.3 kg·hm−2, which was 1.50% higher than that of control. The hybrid inherited 7 blast-resistance and two virus-resistance genes from the parents rendering it blast-free in the field and laboratory for 2 years. Conclusion The rates of brown and milled rice of Yexiangyou 699 (now officially identified as Min Approved Rice No. 20200012) were 80.1% and 68.5%, respectively. The grain quality indicators of the hybrid included 0.8% on chalkiness, Grade 1 on transparency, 5.5 on alkali elimination, 86 mm on gelatinized consistency index, 14.0% on amylopectin content, 3rd grade on the high-quality edible rice set by the Ministry, and high yield and strong disease-resistance in the field. Yexiangyou 699 was recommended as a new breed for cultivation. -
Key words:
- Rice blast resistant /
- hybrid rice /
- Yexiangyou 699 /
- high quality
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图 3 野香优699的父、母本及其杂种F1的背景、籼粳片段分析
A、B、C为基因组背景纯度,D、E、F为籼粳片段分析;FA-1为野香A,FA-3为福恢699,FA-5为野香优699。
Figure 3. Background and Indica japonica fragments of parents and hybrid F1 of Yexiangyou 699
A, B, and C: genomic background purity; D, E, and F: I. japonica fragments; FA-1: Yexiang A; FA-3: Fuhui 699; FA-5: Yexiangyou 699.
表 1 2017—2019年野香优699在福建省中稻区域试验及生产试验中的产量
Table 1. Yield of Yexiangyou 699 in regional and production trials of mid-season rice in Fujian from 2017 to 2019
试验组别
Type of trial年份
Year品种
Varieties生育期
Growth period/d生育期比CK±
Compared with CK/d产量
Yield/(kg·hm−2)增产率
Ratio compared with CK/%区域试验
Regional trial2017 野香优699
Yexiangyou 699141.3 −2.0 9723.3 a −0.20 II优3301
II you 3301(CK)143.3 0 9742.8 a 0 2018 野香优699
Yexiangyou 699139.5 -3.6 9085.4 b 3.20 II优3301
II you 3301(CK)143.1 0 8803.6 a 0 生产试验
Producing trial2019 野香优699
Yexiangyou 699140.4 −2.8 8574.0 10.11 II优3301
II you 3301(CK)143.2 0 7786.8 0 同列数据后不同小写字母表示同一年份不同处理间差异显著(P<0.05)。
Data with different lowercase letters on the same column indicate significant differences between different treatments in the same year (P<0.05).表 2 野香优699稻瘟病抗性鉴定结果
Table 2. Identification on rice blast disease resistance of Yexiangyou 699
年份
Year地点
location田间鉴定
Identification in field室内鉴定
Identification in
greenhouse抗性综评
Comprehensive
evaluation of
resistance叶瘟
Leaf blast穗颈瘟
Panicle blast综评
Comprehensive
evaluation发病率
Incidence
rate%病情指数
Disease
index最高级
Top
grade抗感表现
Resistance
evaluation发病率
Incidence
rate%发病率
病级
Grade of
Incidence
rate病情指数
Disease
index最高级
Top
grade抗感表现
Resistance
evaluation抗菌株率
Resistance to
strain rate/
%抗感表现
Resistance
evaluation2017 将乐
Jiangle1.5 0.22 2 R 14.00 5 1.56 1 R R 94.45 R R 宁化
Ninghua2.00 0.22 1 R 0.00 1 0.00 0 R R 上杭
Shanghang6.50 1.83 3 R 4.50 1 1.39 5 R R 建阳
Jianyang0.00 0.00 0 R 0.00 1 0.00 5 R R 综合
Average2.25 0.54 3 R 4.63 1 0.74 5 R R 2018 将乐
Jiangle38.50 7.33 2 MR 19.00 5 3.00 3 R MR 93.75 R R 宁化
Ninghua3.00 0.39 2 R 2.50 1 0.94 5 R R 上杭
Shanghang5.50 1.56 3 R 5.50 3 1.50 5 R R 建阳
Jianyang0.00 0.00 0 R 21.00 5 5.67 3 MR MR 综合
Average11.75 2.32 3 R 12.00 5 2.78 5 R R 表 3 野香优699的父、母本及其杂种F1的籼粳片段统计分析
Table 3. Statistical analysis on I. Japonica fragments of parents and hybrid F1 of Yexiangyou 699
品种
Varieties区段总数
Total fragments粳稻区段数
Japonica fragments粳稻比例
Japonica proportion/%籼稻区段数
Indica fragments籼稻比例
Indica proportion/%不能判断区段数
Undetermine fragments野香A
Yexiang A750 76 10.13 523 69.73 151 福恢699
Fuhui 699750 78 10.40 513 68.40 159 野香优699
Yexiangyou 699750 77 10.27 565 75.33 108 表 4 野香优699及其亲本的抗病基因检测
Table 4. Detection of disease resistance genes in Yexiangyou 699 and parents
序号
No基因名称
Gene探针类型
Probe类型
Type染色体
Chr对照品种
Standard variety基因功能
Gene function鉴定品种
Varieties野香A
Yexiang A福恢699
Fuhui 699野香优699
Yexiangyou 6991 Rymv1 SNP 抗生物逆境 4 Nipponbare 抗黄色斑驳病毒病 √ √ √ 2 STV11 SNP 抗生物逆境 11 Kasalath 抗水稻条叶枯病毒 √ √ √ 3 Pi1 单倍型 抗生物逆境 11 10SNP 稻瘟病抗性 √ H 4 Pi5 单倍型 抗生物逆境 9 33SNP 稻瘟病抗性 √ H 5 Pi9 单倍型 抗生物逆境 6 80SNP 稻瘟病抗性 √ H 6 Pia 单倍型 抗生物逆境 11 13SNP 稻瘟病抗性 √ H 7 Pid2 单倍型 抗生物逆境 6 66SNP 稻瘟病抗性 √ √ √ 8 Pid3 单倍型 抗生物逆境 6 64SNP 稻瘟病抗性 √ √ √ 9 Pita 单倍型 抗生物逆境 12 32SNP 稻瘟病抗性 √ H “√”表示所检测品种的基因型与对照品种基因型一致;“H”表示品种中该基因为杂合型。
'√' indicates genotype of tested variety to be consistent with that of control;'H' indicates heterozygous gene. -
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