双孢蘑菇9个杂交菌株的蛋白质营养评价与分析

郭仲杰; 郑惠清; 蔡志欣; 卢园萍; 曾志恒; 廖剑华; 陈美元

doi:10.19303/j.issn.1008-0384.2023.11.004

双孢蘑菇9个杂交菌株的蛋白质营养评价与分析

doi: 10.19303/j.issn.1008-0384.2023.11.004

福建省农业科学院食用菌研究所/特色食用菌繁育与栽培国家地方联合工程研究中心，福建　福州　350012

基金项目: 福建省科技计划公益类专项（2021R1035006、2022R1035006）；国家现代农业产业技术体系建设专项（CARS-20）；福建省种业创新与产业化工程项目（zycxny2021011）

详细信息

作者简介:
郭仲杰（1974 —），男，硕士，主要从事食用菌育种研究，E-mail：550410857@qq.com

郑惠清（1993 —），女，硕士，主要从事食用菌育种研究，E-mail：1132055710@qq.com

通讯作者:
陈美元（1972 —），男，博士，教授级高级工程师，主要从事食用菌遗传育种研究，E-mail: 401061326@qq.com

中图分类号: S646.11
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出版历程
- 收稿日期: 2023-07-03
- 修回日期: 2023-08-23
- 网络出版日期: 2023-11-20
- 刊出日期: 2023-11-28

Protein Nutritional Value of Nine Agaricus bisporus Hybrids

Edible Fungi Institute, Fujian Academy of Agricultural Sciences/National and Local Joint Engineering Research Center for Breeding & Cultivation of Featured Edible Fungi, Fuzhou, Fujian　350012, China

摘要

摘要: 目的分析评价双孢蘑菇野生种质创新杂交菌株的蛋白质和氨基酸营养成分，为利用野生种质选育营养价值更高的双孢蘑菇新菌株提供参考。方法以3个主栽品种为对照，测定分析了6个杂交新菌株的蛋白质与氨基酸含量，并参照WHO（World Health Organization，世界卫生组织）/FAO（Food and Agriculture Organization of the United Nations，联合国粮食及农业组织）/UNU（United Nations University，联合国大学）、IOM（Institute of Medicine，美国医学研究所）、全鸡蛋蛋白3种氨基酸平衡模式谱，采用IOM模式评分、氨基酸评分、氨基酸比值系数、氨基酸比值系数分、化学评分等多种指标进行综合评价。结果野生种质创新杂交白色菌株福蘑63每百克蛋白质的必需氨基酸含量46.76 g、必需氨基酸指数94.45、必需氨基酸比例41.72%、消化率校正氨基酸得分86.73，在9个菌株中都是最高的。而野生种质创新杂交棕色菌株的蛋白质、总氨基酸、必需氨基酸、药用氨基酸、甜味氨基酸、碱性氨基酸、支链氨基酸以及多种氨基酸的含量均显著高于主栽品种。结论现有双孢蘑菇白色主栽品种的蛋白质、氨基酸等营养成分仍有提升的空间，可以引入营养价值相对较高的野生种质资源，基于营养成分评价开展营养品质定向杂交育种。
- 双孢蘑菇 /
- 野生种质创新 /
- 杂交新菌株 /
- 营养评价
Abstract: Objective Proteins in 6 newly bred hybrids of wild Agaricus bisporus were analyzed for nutritional value to aid breeding selection. Method Along with 3 major A. bisporus varieties currently cultivated in Fujian as reference, 6 hybrids of wild germplasms were analyzed to determine their nutritional values based on protein and AA compositions. Scores of IOM, AAs, and chemistry, as well as AA ratio coefficient of the samples were evaluated with respect to the balanced AA spectra of WHO/FAO/UNU, IOM, and egg protein. Result Among the various species of mushrooms, Fumo 63 was found to have the highest essential AA content of 46.76 g·100 g⁻¹protein, essential AA index of 94.45, essential AAs proportion of 41.72%, and adjusted AA digestibility of 86.73. All 6 hybrids contained significantly more protein and total, essential, medicinal, sweet, alkaline, and branched-chain AAs than did their brown wild A. bisporus parents. Conclusion There appeared to be ample rooms for nutritional improvements by hybridizing the wild A. bisporus through upgrading the protein quality of the mushroom.
- Agaricus bisporus /
- wild germplasms /
- new hybrid strains /
- nutritional evaluation

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表 1 样品信息

Table 1. Relevant information on specimens

菌株编号 Code	菌株名称 Name	亲本 Parents
1	As2796	02×8213
2	W192	02×As2796
3	A15	U1(Somycel9.2×Somycel53)
4	福蘑48 Fumo 48	As2796×A15
5	福蘑52 Fumo 52	W192×A15
6	福蘑63 Fumo 63	(ARP159×AGQG841)×(02×As2796)
7	福蘑B07 Fumo B07	Ag781314×As2796
8	福蘑B08 Fumo B08	ARP088×As2796
9	福蘑B15 Fumo B15	(ARP159×AGQL8133)×As2796

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表 2 9份双孢蘑菇蛋白质营养成分

Table 2. Nutritional compositions on proteins of 9 A. bisporus strains

营养成分 Nutrient content	含量 Content/%
营养成分 Nutrient content	1**	2	3	4	5	6	7	8	9
蛋白质 Protein	36.70	33.50	32.10	35.90	35.10	35.50	37.90	46.30	40.00
天冬氨酸 Asp	2.58	2.38	2.17	2.41	2.51	2.62	2.49	3.30	2.74
苏氨酸 Thr*	1.33	1.22	1.17	1.33	1.31	1.42	1.37	1.60	1.61
丝氨酸 Ser	1.27	1.22	1.15	1.24	1.28	1.38	1.32	1.58	1.52
谷氨酸 Glu	6.15	4.80	5.30	6.39	5.76	4.29	6.18	7.38	6.20
甘氨酸 Gly	1.34	1.26	1.17	1.34	1.35	1.39	1.29	1.60	1.54
丙氨酸 Ala	2.65	2.53	2.43	2.51	2.41	2.53	2.49	3.22	2.91
缬氨酸 Val*	1.45	1.31	1.25	1.44	1.44	1.52	1.48	1.74	1.75
甲硫氨酸 Met*	2.79	2.34	2.61	2.88	2.80	2.68	2.94	2.77	3.00
异亮氨酸 Ile*	1.20	1.10	1.06	1.20	1.23	1.26	1.20	1.44	1.37
亮氨酸 Leu*	1.91	1.73	1.65	1.88	1.92	1.96	1.85	2.11	2.11
酪氨酸 Tyr	0.79	0.80	0.67	0.74	0.74	0.81	0.79	0.86	0.91
苯丙氨酸 Phe *	1.17	1.07	1.03	1.16	1.18	1.26	1.18	1.34	1.38
赖氨酸 Lys *	1.46	1.37	1.02	1.44	1.48	1.56	1.51	1.78	1.86
组氨酸 His	0.56	0.53	0.49	0.55	0.57	0.60	0.58	0.62	0.70
精氨酸 Arg	1.38	1.36	1.20	1.34	1.45	1.45	1.37	1.57	1.48
脯氨酸 Pro	1.22	1.58	1.49	1.17	1.33	1.22	1.05	2.87	1.21
氨基酸总量 TAA	29.25	26.60	25.86	29.02	28.76	27.95	29.09	35.78	32.29
必需氨基酸总量 EAA	11.31	10.14	9.79	11.33	11.36	11.66	11.53	12.78	13.08
非必需氨基酸总量 NEAA	17.94	16.46	16.07	17.69	17.40	16.29	17.56	23.00	19.21
EAA/TAA/%	38.67	38.12	37.86	39.04	39.50	41.72	39.64	35.72	40.51
EAA/NEAA/%	63.04	61.60	60.92	64.05	65.29	71.58	65.66	55.57	68.09
药用氨基酸总量 MAA	19.57	17.11	16.82	19.58	19.19	18.02	19.60	22.71	21.22
MAA/TAA /%	66.91	64.32	65.04	67.47	66.72	64.47	67.38	63.47	65.72
鲜味氨基酸总量 Total tasty amino acid	8.73	7.18	7.47	8.80	8.27	6.91	8.67	10.68	8.94
甜味氨基酸总量 Total sweet amino acid	6.59	6.23	5.92	6.42	6.35	6.72	6.47	8.00	7.58
支链氨基酸总量 Total branched chain amino acid	4.56	4.14	3.96	4.52	4.59	4.74	4.53	5.29	5.23
碱性氨基酸总量 Total basic amino acid	3.40	3.26	2.71	3.33	3.50	3.61	3.46	3.97	4.04
为必需氨基酸，表3同。1~9为菌株编号，表4~8同。 : Essential amino acid，The same as the Table 3. **：1-9 are strain codes， The same as the Table4-8.

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表 3 9份双孢蘑菇氨基酸含量统计参数

Table 3. Statistical parameters on AAs of 9 A. bisporus strains

氨基酸 Amino acid	平均值 Mean/%	变幅 Range/%	变异系数 CV/%
Asp	2.58±0.30	2.17~3.30	11.53
Thr*	1.37±0.14	1.17~1.61	10.36
Ser	1.33±0.13	1.15~1.58	10.03
Glu	5.83±0.87	4.29~7.38	14.91
Gly	1.36±0.13	1.17~1.60	9.23
Ala	2.63±0.25	2.41~3.22	9.56
Val*	1.49±0.16	1.25~1.75	10.70
Met*	2.76±0.19	2.34~3.00	6.76
Ile*	1.23±0.11	1.06~1.44	9.12
Leu*	1.90±0.14	1.65~2.11	7.57
Tyr	0.79±0.07	0.67~0.91	8.35
Phe*	1.20±0.11	1.03~1.38	9.01
Lys*	1.50±0.23	1.02~1.86	15.17
His	0.58±0.06	0.49~0.70	9.71
Arg	1.40±0.10	1.20~1.57	7.01
Pro	1.46±0.52	1.05~2.87	35.74

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表 4 9份双孢蘑菇蛋白质的必需氨基酸含量

Table 4. Essential AA contents in proteins of 9 A. bisporus strains 　　　　　　　　　　　　　　（单位：%）

编号 Code	His	Thr	Lys	Leu	Ile	Met	Phe+Tyr	Val	合计 Total
1	1.91	4.55	4.99	6.53	4.10	9.54	6.70	4.96	43.28
2	1.99	4.59	5.15	6.50	4.14	8.80	7.03	4.92	43.12
3	1.89	4.52	3.94	6.38	4.10	10.09	6.57	4.83	42.34
4	1.90	4.58	4.96	6.48	4.14	9.92	6.55	4.96	43.49
5	1.98	4.55	5.15	6.68	4.28	9.74	6.68	5.01	44.05
6	2.15	5.08	5.58	7.01	4.51	9.59	7.41	5.44	46.76
7	1.99	4.71	5.19	6.36	4.13	10.11	6.77	5.09	44.35
8	1.73	4.47	4.97	5.90	4.02	7.74	6.15	4.86	39.85
9	2.17	4.99	5.76	6.53	4.24	9.29	7.09	5.42	45.49
A	1.50	2.30	4.50	5.90	3.00	2.20	3.80	3.90	27.10
B	1.80	2.70	5.10	5.50	2.50	2.50	4.70	3.20	28.00
C	2.20	4.70	7.00	8.60	5.40	5.70	9.30	6.60	49.50
A为WHO/FAO/UNU于2007提出的氨基酸平衡模式谱；B为IOM于2005提出的氨基酸平衡模式谱；C为全鸡蛋蛋白模式谱。 A: Spectrum of balanced AA of WHO/FAO/UNU model, 2007; B: 2005 IOM model; C: spectrum of egg white protein.

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表 5 9份双孢蘑菇蛋白质的IOM模式评分

Table 5. IOM scores on 9 A. bisporus strains

编号 Code	His	Thr	Lys	Leu	Ile	Met	Phe+Tyr	Val
1	106.11	168.52	97.84	118.73	164.00	381.60	142.55	155.00
2	110.56	170.00	100.98	118.18	165.60	352.00	149.57	153.75
3	105.00	167.41	77.25	116.00	164.00	403.60	139.79	150.94
4	105.56	169.63	97.25	117.82	165.60	396.80	139.36	155.00
5	110.00	168.52	100.98	121.45	171.20	389.60	142.13	156.56
6	119.44	188.15	109.41	127.45	180.40	383.60	157.66	170.00
7	110.56	174.44	101.76	115.64	165.20	404.40	144.04	159.06
8	96.11	165.56	97.45	107.27	160.80	309.60	130.85	151.88
9	120.56	184.81	112.94	118.73	169.60	371.60	150.85	169.38
平均值 Mean	109.32	173.00	99.54	117.92	167.38	376.98	144.09	157.95

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表 6 双孢蘑菇蛋白质的氨基酸评分

Table 6. AA scores on protein of A. bisporus

Code	His	Thr	Lys	Leu	Ile	Met	Phe+Tyr	Val	PDCAAS
1	127.33	197.83	110.89	110.68	136.67	433.64	176.32	127.18	80.80
2	132.67	199.57	114.44	110.17	138.00	400.00	185.00	126.15	80.42
3	126.00	196.52	87.56	108.14	136.67	458.64	172.89	123.85	63.92
4	126.67	199.13	110.22	109.83	138.00	450.91	172.37	127.18	80.18
5	132.00	197.83	114.44	113.22	142.67	442.73	175.79	128.46	82.65
6	143.33	220.87	124.00	118.81	150.33	435.91	195.00	139.49	86.73
7	132.67	204.78	115.33	107.80	137.67	459.55	178.16	130.51	78.69
8	115.33	194.35	110.44	100.00	134.00	351.82	161.84	124.62	73.00
9	144.67	216.96	128.00	110.68	141.33	422.27	186.58	138.97	80.80
平均值 Mean	131.19	203.09	112.81	109.92	139.48	428.38	178.22	129.60	78.58

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表 7 9份双孢蘑菇蛋白质的氨基酸比值系数和氨基酸比值系数分

Table 7. AA ratio coefficients of 9 A. bisporus strains

项目 Item	必需氨基酸 EAA	1	2	3	4	5	6	7	8	9	平均值 Mean
氨基酸比值系数RC	His	0.72	0.75	0.72	0.71	0.73	0.75	0.72	0.71	0.78	0.73
	Thr	1.11	1.13	1.12	1.11	1.09	1.16	1.12	1.20	1.17	1.13
	Lys	0.62	0.65	0.50	0.62	0.63	0.65	0.63	0.68	0.69	0.63
	Leu	0.62	0.63	0.61	0.61	0.63	0.62	0.59	0.62	0.60	0.61
	Ile	0.77	0.78	0.78	0.77	0.79	0.79	0.75	0.83	0.76	0.78
	Met	2.44	2.27	2.61	2.52	2.45	2.28	2.51	2.17	2.27	2.39
	Phe+Tyr	0.99	1.05	0.98	0.96	0.97	1.02	0.97	1.00	1.00	0.99
	Val	0.71	0.72	0.70	0.71	0.71	0.73	0.71	0.77	0.75	0.72
比值系数分SRC		43.25	48.89	36.63	40.42	43.23	48.58	40.59	52.22	49.09	44.77

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表 8 9份双孢蘑菇蛋白质的化学评分

Table 8. Chemical scores on 9 A. bisporus strains

必需氨基酸 EAA	化学评分 (CS)
必需氨基酸 EAA	1	2	3	4	5	6	7	8	9
His	86.82	90.45	85.91	86.36	90.00	97.73	90.45	78.64	98.64
Thr	96.81	97.66	96.17	97.45	96.81	108.09	100.21	95.11	106.17
Lys	71.29	73.57	56.29	70.86	73.57	79.71	74.14	71.00	82.29
Leu	75.93	75.58	74.19	75.35	77.67	81.51	73.95	68.60	75.93
Ile	75.93	76.67	75.93	76.67	79.26	83.52	76.48	74.44	78.52
Met	167.37	154.39	177.02	174.04	170.88	168.25	177.37	135.79	162.98
Phe+Tyr	72.04	75.59	70.65	70.43	71.83	79.68	72.80	66.13	76.24
Val	75.15	74.55	73.18	75.15	75.91	82.42	77.12	73.64	82.12
EAAI	86.49	86.98	83.65	86.63	88.24	94.45	88.56	80.67	92.24

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表 9 不同双孢蘑菇菌株蛋白质营养成分含量比较

Table 9. Nutritional compositions of different strains of A. bisporus　　　　　　　　　　　　　（单位：%）

营养成分 Nutrient content	主栽品种均值 Main cultivar mean	杂交白色菌株均值 White strain mean	杂交棕色菌株均值 Brown strain mean
蛋白质 Protein	34.10±1.93 a	35.50±4.61 a	41.40±3.57 b
Asp	2.38±0.17	2.51±8.61	2.84±0.34
Thr*	1.24±0.07 a	1.35±5.61 ab	1.53±0.11 b
Ser	1.21±0.05 a	1.30±4.61 ab	1.47±0.11 b
Glu	5.42±0.56	5.48±5.61	6.59±0.56
Gly	1.26±0.07 a	1.36±4.61 ab	1.48±0.13 b
Ala	2.54±0.09	2.48±8.61	2.87±0.30
Val*	1.34±0.08 a	1.47±6.61 ab	1.66±0.12 b
Met*	2.58±0.18 a	2.79±9.61 ab	2.90±0.10 b
Ile*	1.12±0.06 a	1.23±3.61 ab	1.34±0.10 b
Leu*	1.76±0.11 a	1.92±0.61 ab	2.02±0.12 b
Tyr	0.75±0.06	0.76±8.61	0.85±0.05
Phe^*	1.09±0.06 a	1.20±3.61 ab	1.30±0.09 b
Lys^*	1.28±0.19 a	1.49±7.61 ab	1.72±0.15 b
His	0.53±0.03 a	0.57±6.61 ab	0.63±0.05 b
Arg	1.31±0.08	1.41±4.61	1.47±0.08
Pro	1.43±0.15	1.24±7.61	1.71±0.82
TAA	27.24±1.46 a	28.58±3.61 ab	32.39±2.73 b
EAA	10.41±0.65 a	11.45±4.61 ab	12.46±0.67 b
NEAA	16.82±0.81	17.13±9.61	19.92±2.28
MAA	17.83±1.23 a	18.93±1.61 ab	21.18±1.27 b
EAA/TAA /%)	38.22±0.34	40.09±6.61	38.62±2.08
EAA/NEAA /%)	61.85±0.88	66.97±1.61	63.11±5.42
MAA/TAA /%)	65.42±1.09	66.22±5.61	65.52±1.60
鲜味氨基酸总量 Total tasty amino acid	7.79±0.67	7.99±4.61	9.43±0.89
甜味氨基酸总量 Total sweet amino acid	6.25±0.27 a	6.50±3.61 ab	7.35±0.65 b
支链氨基酸总量 Total branched chain amino acid	4.22±0.25 a	4.62±0.61 ab	5.02±0.34 b
碱性氨基酸总量 Total basic amino acid	3.12±0.30 a	3.48±8.61 ab	3.82±0.26 b
不同小写字母表示不同菌种类别之间差异显著（P＜0.05）。 a and b indicate significant differences at P＜0.05.

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参考文献(29)

[1]	王贺祥, 刘庆洪. 食用菌栽培手册[M]. 北京: 中国农业大学出版社, 2015.
[2]	吴素玲, 孙晓明, 王波, 等. 双孢蘑菇子实体营养成分分析 [J]. 中国野生植物资源, 2006, 25(2):47−48,52. WU S L, SUN X M, WANG B, et al. Analysis of main nutrition components in Agaricus brunnescens fruitbodies [J]. Chinese Wild Plant Resources, 2006, 25(2): 47−48,52.(in Chinese)
[3]	廖剑华, 陈美元, 郭仲杰, 等. 双孢蘑菇种质创新与新品种W192等的选育及应用 [J]. 中国科技成果, 2021, 22(9):6−7. LIAO J H, CHEN M Y, GUO Z J, et al. Germplasm innovation of Agaricus bisporus and breeding and application of new variety W192 [J]. China Science and Technology Achievements, 2021, 22(9): 6−7.(in Chinese)
[4]	王泽生, 廖剑华, 陈美元, 等. 双孢蘑菇遗传育种和产业发展 [J]. 食用菌学报, 2012, 19(3):1−14. WANG Z S, LIAO J H, CHEN M Y, et al. Breeding and industrial development of Agaricus bisporus [J]. Acta Edulis Fungi, 2012, 19(3): 1−14.(in Chinese)
[5]	ROKNI N, ALI AKBAR SADATI S, SAFAIE N, et al. Assessment of genetic relatedness among commercial and wild strains of Agaricus bisporus using repetitive extragenic palindromic sequences and polymerase chain reaction [J]. Mycoscience, 2019, 60(5): 293−297. doi: 10.1016/j.myc.2019.06.002
[6]	李洪荣, 廖剑华, 陈美元, 等. 双孢蘑菇种质资源生物学特性分析[G]//中国菌物学会2014年学术年会论文摘要集. 2014-11-6.
[7]	郭仲杰, 蔡志欣, 曾志恒, 等. 棕色蘑菇杂交新品种的选育 [J]. 福建轻纺, 2021(5):11−15. GUO Z J, CAI Z X, ZENG Z H, et al. Breeding of new hybrid varieties of brown mushroom [J]. The Light & Textile Industries of Fujian, 2021(5): 11−15.(in Chinese)
[8]	蔡志欣, 陈美元, 廖剑华, 等. 部分中国野生双孢蘑菇的DNA鉴定和遗传分析 [J]. 福建农业学报, 2011, 26(2):248−253. CAI Z X, CHEN M Y, LIAO J H, et al. DNA identification and genetic analysis on wild Agaricus bisporus strains in China [J]. Fujian Journal of Agricultural Sciences, 2011, 26(2): 248−253.(in Chinese)
[9]	廖剑华. 双孢蘑菇野生种质杂交育种研究Ⅰ [J]. 中国农学通报, 2013, 29(7):93−98. LIAO J H. Study on the crossbreeding using wild strains of Agaricus bisporus Ⅰ [J]. Chinese Agricultural Science Bulletin, 2013, 29(7): 93−98.(in Chinese)
[10]	WANG Z C, LI M R, FAN J D, et al. Cultivation and nutritional evaluation of Agaricus bisporus with tea residue as culture medium [J]. Foods, 2023, 12(13): 2440. doi: 10.3390/foods12132440
[11]	BĄKOWSKI J, KOSSON R. Nutritional value and amino acids composition of the mushroom (Agaricus bisporus) at different stages of its development [J]. Acta Agrobotanica, 2013, 38(2): 103−113. doi: 10.5586/aa.1985.009
[12]	BĄKOWSKI J, KOSSON R, HORBOWICZ M. Nutritional values of different strains of mushrooms (Agaricus bisporus) [J]. Acta Agrobotanica, 2013, 39(1): 111−121. doi: 10.5586/aa.1986.010
[13]	IDREES M, YAQOOB S, LEO S F, et al. A comparison of the physical, chemical, and structural properties of wild and commercial strains of button mushroom, Agaricus bisporus (agaricomycetes) [J]. International Journal of Medicinal Mushrooms, 2019, 21(6): 611−625. doi: 10.1615/IntJMedMushrooms.2019030681
[14]	PAULAUSKIENĖ A, TARASEVIČIENĖ Ž, ŠILEIKIENĖ D, et al. The quality of ecologically and conventionally grown white and brown Agaricus bisporus mushrooms [J]. Sustainability, 2020, 12(15): 6187. doi: 10.3390/su12156187
[15]	郑惠清, 郭仲杰, 蔡志欣, 等. 双孢蘑菇野生种质资源营养成分分析与评价 [J]. 生物技术通报, 2021, 37(11):109−118. ZHENG H Q, GUO Z J, CAI Z X, et al. Analysis and evaluation of nutrient components in Agaricus bisporus wild germplasm resource [J]. Biotechnology Bulletin, 2021, 37(11): 109−118.(in Chinese)
[16]	国家卫生和计划生育委员会, 国家食品药品监督管理总局. 食品安全国家标准食品中蛋白质的测定: GB 5009.5—2016[S]. 北京: 中国标准出版社, 2017.
[17]	国家卫生和计划生育委员会, 国家食品药品监督管理总局. 食品安全国家标准食品中氨基酸的测定: GB 5009.124—2016[S]. 北京: 中国标准出版社, 2017.
[18]	陈洪雨, 鲍大鹏, 康前进, 等. 5种市售工厂化栽培杏鲍菇的氨基酸组成及蛋白质营养分析 [J]. 上海农业学报, 2019, 35(4):9−15. CHEN H Y, BAO D P, KANG Q J, et al. Amino acid composition and protein nutrition analysis of 5 commercial industrial cultivated Pleurotus eryngii samples [J]. Acta Agriculturae Shanghai, 2019, 35(4): 9−15.(in Chinese)
[19]	WHO/FAO/UNU. Protein and amino acid requirements in human nutrition report of a joint WHO/FAO/UNU expert consultation[M]. Geneva: World Health Organization, 2007.
[20]	IOM. Dietary reference intakes for energy, carbohydrate, fiber, fat, fatty acids, cholesterol, protein, and amino acids[M]. Washington, D. C. : National Academies Press, 2005.
[21]	SELIGSON F H, MACKEY L N. Variable predictions of protein quality by chemical score due to amino acid analysis and reference pattern [J]. The Journal of Nutrition, 1984, 114(4): 682−691. doi: 10.1093/jn/114.4.682
[22]	BOYE J, WIJESINHA-BETTONI R, BURLINGAME B. Protein quality evaluation twenty years after the introduction of the protein digestibility corrected amino acid score method [J]. The British Journal of Nutrition, 2012, 108(Suppl 2): S183−S211.
[23]	朱圣陶, 吴坤. 蛋白质营养价值评价: 氨基酸比值系数法 [J]. 营养学报, 1988, 10(2):187−190. ZHU S T, WU K. Nutritional evaluation of protein-ratio coefficient of amino acid [J]. Acta Nutrimenta Sinica, 1988, 10(2): 187−190.(in Chinese)
[24]	OSER B L. Method for integrating essential amino acid content in the nutritional evaluation of protein [J]. Journal of the American Dietetic Association, 1951, 27(5): 396−402. doi: 10.1016/S0002-8223(21)30758-1
[25]	张晓煜, 刘静, 袁海燕, 等. 不同地域环境对枸杞蛋白质和药用氨基酸含量的影响 [J]. 干旱地区农业研究, 2004, 22(3):100−104. ZHANG X Y, LIU J, YUAN H Y, et al. Effect of surrounding conditions on protein and pharmacological amino acid of Lycium barbarum L [J]. Agricultural Research in the Arid Areas, 2004, 22(3): 100−104.(in Chinese)
[26]	陈宏, 章骞, 洪水河, 等. 双孢蘑菇预煮液成分分析及其降血压效果 [J]. 集美大学学报(自然科学版), 2018, 23(3):171−177. CHEN H, ZHANG Q, HONG S H, et al. Analysis of the components of pre-boiled liquid of Agaricus bisporus and its effect on antihypertension [J]. Journal of Jimei University (Natural Science), 2018, 23(3): 171−177.(in Chinese)
[27]	贺洪, 刘慧敏. 碱性氨基酸对大强度运动后机体的保护作用 [J]. 湘潭师范学院学报(自然科学版), 2008, 30(4):30−32. HE H, LIU H M. Protective effect of basic amino acids on body after intensive exercise [J]. Journal of Xiangtan Normal University (Natural Science Edition), 2008, 30(4): 30−32.(in Chinese)
[28]	刘宗奇, 武晨剑, 袁学文, 等. 白色和黄色金针菇子实体营养成分与挥发性物质比较 [J]. 食用菌学报, 2021, 28(3):102−111. LIU Z Q, WU C J, YUAN X W, et al. Comparison of nutrients and volatile compounds between fruiting bodies of white and yellow Flammulina filiformis [J]. Acta Edulis Fungi, 2021, 28(3): 102−111.(in Chinese)
[29]	吴莹莹, 鲍大鹏, 李燕, 等. 蟹味菇和白玉菇的蛋白质及氨基酸营养评价与分析 [J]. 上海农业学报, 2021, 37(1):13−21. WU Y Y, BAO D P, LI Y, et al. Evaluation and analysis of protein and amino acid nutrition of buna-shimeji and bunapi-shimeji [J]. Acta Agriculturae Shanghai, 2021, 37(1): 13−21.(in Chinese)