纤维素降解菌的分离筛选及产酶条件优化

张慧; 赵恪; 林陈强; 林戎斌; 陈龙军; 陈济琛

doi:10.19303/j.issn.1008-0384.2023.09.014

纤维素降解菌的分离筛选及产酶条件优化

doi: 10.19303/j.issn.1008-0384.2023.09.014

1.
福建省农业科学院土壤肥料研究所，福建　福州　350013
2.
福建农林大学资源与环境学院，福建　福州　350002

基金项目: 福建省科技计划公益类专项（2023R1074）；福州市农业科技项目（2020-N-12）；福建省科技计划项目（2020I0045）；福建省农业科学院科技创新团队建设项目（CXTD2021030）

详细信息

作者简介:
张慧（1981 —），女，硕士，助理研究员，主要从事农业微生物研究，E-mail：zhanghui08@126.com

通讯作者:
陈济琛（1965 — ），男，研究员，主要从事微生物研究，E-mail：chenjichen2001@126.com

中图分类号: X705
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- 文章访问数: 402
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- 被引次数: 0
出版历程
- 收稿日期: 2022-05-23
- 修回日期: 2023-07-26
- 网络出版日期: 2023-10-25
- 刊出日期: 2023-09-28

Isolation and Enzymatic Activity of Cellulose-degrading Bacteria

1.
Soil and Fertilizer Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 　350013, China
2.
College of Resources and Environment, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

摘要

摘要: 目的为获得秸秆降解的应用型菌株，自制秸秆腐熟菌剂。方法从林下土壤和堆肥样品中，采用羧甲基纤维素钠和刚果红培养基进行分离筛选，并对菌株纤维素酶活性、滤纸酶活性和木聚糖酶活性进行测定。结果获得1株羧甲基纤维素酶活力最高的菌株，经形态学和分子生物学鉴定为地衣芽孢杆菌（Bacillus licheniformis）。该菌株对滤纸有崩解效果，滤纸酶活性14.21 U·mL⁻¹，木聚糖酶活性24.03 U·mL⁻¹，玉米秸秆降解率为21.2%。结论菌株TB1滤纸崩解试验效果较好，纤维素酶活性、滤纸酶活性和木聚糖酶活性均较高，具有秸秆降解开发应用价值。
- 纤维素降解菌 /
- 秸秆 /
- 酶活
Abstract: Objective Bacteria capable of effectively degrading spent straws were isolated, and conditions for the enzymatic reaction analyzed. Method On a medium containing sodium carboxymethyl cellulose and Congo red, potential strains capable of decomposing fibrous material were isolated from sampled forest soil and compost. Enzymatic activities of the isolates were examined, and conditions determined. Result Bacillus licheniformis was identified as the strain with the greatest carboxymethyl cellulase activity. It demonstrated a filter paper degrading activity of 14.21 U·mL⁻¹, a xylanase activity of 24.03 U·mL⁻¹, and an ability to decompose 21.2% of corn straws. On corn straws, the selected isolate was observed to show the maximum digestion with an overall activity up to 48.63 U·mL⁻¹ by using KNO₃ for nitrogen source at pH 7 and 30 ℃. Conclusion B. licheniformis was a cellulose-degrading bacterium that could be used to decompose spent corn straws for waste disposal.
- Cellulose-degrading bacteria /
- corn straw /
- enzymatic properties

HTML全文

图 1 菌株TB1在刚果红培养基上的水解效果

Figure 1. Hydrolysis on Congo red medium by TB 1 strain

下载: 全尺寸图片幻灯片

图 2 TB1菌株的滤纸崩解效果

左瓶为加TB1菌液处理，右瓶为对照。

Figure 2. Degradation of filter paper by TB1

Bottle on left contains filter paper treated with TB1 broth; that on right, blank control.

下载: 全尺寸图片幻灯片

图 3 纤维素降解菌株16S rRNA序列系统发育树

Figure 3. Phylogenetic tree of 16S rRNA sequence of cellulose-degrading bacteria

下载: 全尺寸图片幻灯片

图 4 不同碳源、氮源、温度和pH对菌株TB1纤维素酶活的影响

不同小写字母表示差异显著(P＜0.05)。

Figure 4. Effects of carbon sources, nitrogen sources, temperature, and pH on cellulase activity of TB1

Data with different lowercase letters indicate significant differences at P＜0.05.

下载: 全尺寸图片幻灯片

表 1 各菌株降解纤维素能力评价

Table 1. Cellulose degradation activities of isolated strains

菌株编号 Strain No.	水解圈直径 Hydrolysis spot diameter/mm	滤纸酶活 Filter paper enzyme activity/ （U·mL⁻¹）	木聚糖酶活 Xylanase activity/ （U·mL⁻¹）	CMC最高酶活 Highest CMCase/ （U·mL⁻¹）	最高酶活出现时间 Time of the highest CMCase/d
TB1	15.40±0.80	14.21±0.24	24.03±0.12	38.69±0.54	3
TB2	8.51±0.91	5.34±0.07	10.07±0.06	16.03±0.11	4
TB3	3.44±0.83	9.46±0.64	12.10±0.15	9.46±0.64	3
TB4	9.52±0.64	4.32±0.05	8.35±0.41	17.10±0.35	5
TB5	10.10±0.44	5.32±0.16	10.94±0.33	14.17±0.22	5
TB6	6.54±0.11	3.32±0.11	4.86±0.16	8.32±0.05	7

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