Mechanism of Red or Blue Light in Regulating Root Development of Tobacco Seedlings
-
摘要:
目的 探究红蓝光质调控烟苗根系发育的机理。 方法 以烤烟品种翠碧1号为试材，采用水培试验，研究红蓝光对烟草幼苗根系生长、内源气体信号分子硫化氢（H2S）合成和积累的影响。 结果 处理7 d时，与对照白光处理相比，结果表明：（1）红光处理烟苗地上部和根系生物量显著增加，增幅分别为74.62%和15.64%，蓝光根系生物量降幅为7.00%；（2）红光处理烟苗根系H2S含量显著增加，增幅为61.72%，而蓝光处理显著降低；（3）红光处理烟苗根系脱巯基酶（DES）、半胱氨酸合成酶（CS）活性分别升高24.28%、25.61%；蓝光处理则分别降低32.10%、18.30%；（4）红光处理的H2S生物合成关键基因NtDES表达量为白光处理的8.8倍，蓝光处理仅为白光处理的13.82%。 结论 红光处理可通过增强H2S生物合成关键酶编码基因的表达提高烟苗根系H2S的含量，进而促进侧根的发生，而蓝光效应与红光相反。 Abstract:Objective Mechanism of red or blue light in regulating the root development of flue-cured tobacco seedlings was studied. Method Effects of red or blue LED light exposure on the root growth and the synthesis and accumulation of endogenous gas signal molecule hydrogen sulfide (H2S) in CB-1 tobacco seedlings were monitored. Results After 7 days of the exposure to red or blue LED in comparison to white light, (1) the biomasses of aboveground parts and roots of the seedlings treated by red light increased by 74.62% and 15.64%, respectively, while the roots of the seedlings exposed to the blue LED decreased by 7.00%; (2) the H2S content on the red light-treated plants increased by 61.72%, but reduced on the blue light-treated counterparts; (3) the red LED increased the activities of DES and CS by 24.28% and 25.61%, respectively, but the blue LED decreased them by 32.10% and 18.30%, respectively; (4) the NtDES expression in the red light-treated seedlings were 8.8-fold of that in the plants treated by white light, whereas the blue light resulted in merely 13.82% of what white light did. Conclusion In contrast to blue LED, the exposure of the tobacco seedlings to red LED rose H2S in the plants through increased expression of the key enzyme coding genes in the biosynthesis inducing a thriving generation of lateral roots. -
Key words:
- Red or blue LED light exposure /
- tobacco /
- lateral root /
- hydrogen sulfide
-
图 2 不同光质对烟苗生长的影响
不同小写字母表示处理间差异有统计学意义（P＜0.05），下同。
Figure 2. Effects of different LEDs on growth of tobacco seedlings
Data with different lowercase letters indicate significant differences at P＜0.05. Same for below.
图 3 不同光质处理对烟苗根系中H2S含量的影响
Figure 3. Effects of different LEDs on H2S content in tobacco seedlings
图 4 不同光质处理对烟苗体内H2S合成关键酶活性的影响
Figure 4. Effects of different LEDs on activities of key enzymes in H2S synthesis of tobacco seedlings
图 5 不同光质处理对NtDES表达量的影响
Figure 5. Effects of different LEDs on NtDES expression in seedlings
图 6 H2S供体和清除剂对烟苗侧根发生的影响
Figure 6. Effects of H2S donor and scavenger on lateral root formation of tobacco seedlings
表 1 NtDES和NtL25引物序列
Table 1. Primers of NtDES and NtL25 for qRT-PCR
基因名称
Gene引物序列
Primer sequenceNtDES F: 5'-AAATAACTGGCAATGGGGTT-3'
R: 5'-TGGTCTGTTGGCGAGTCTAA-3'NtL25 F: 5'-AGTCTTAGGTCGGTGGA-3'
R: 5'-AGCTGGTAGATTTCTCGT-3'
下载: 导出CSV
-
[1] 时向东, 王林枝, 满晓丽, 等. 不同光质对烤烟漂浮育苗中烟苗生长发育及光合特性的影响 [J]. 中国烟草学报, 2013, 19(1):43−46. doi: 10.3969/j.issn.1004-5708.2013.01.009SHI X D, WANG L Z, MAN X L, et al. Effects of different light qualities on growth and development and photosynthetic physiological characteristics of flue-cured tobacco seedlings in floating system [J]. Acta Tabacaria Sinica, 2013, 19(1): 43−46.(in Chinese) doi: 10.3969/j.issn.1004-5708.2013.01.009 [2] LEE H J, PARK Y J, HA J H, et al. Multiple routes of light signaling during root photomorphogenesis [J]. Trends in Plant Science, 2017, 22(9): 803−812. doi: 10.1016/j.tplants.2017.06.009 [3] YANG Y, LIU H T. Coordinated shoot and root responses to light signaling in Arabidopsis [J]. Plant Communications, 2020, 1(2): 100026. doi: 10.1016/j.xplc.2020.100026 [4] 孟霖, 徐宜民, 宋文静, 等. 红蓝单色光对水培烟草幼苗生长发育及生理特性的影响 [J]. 中国烟草学报, 2015, 21(5):55−61. doi: 10.16472/j.chinatobacco.2015.001MENG L, XU Y M, SONG W J, et al. Effects of red and blue monochromatic lights on growth, development and physiological characters of hydroponic tobacco seedlings [J]. Acta Tabacaria Sinica, 2015, 21(5): 55−61.(in Chinese) doi: 10.16472/j.chinatobacco.2015.001 [5] VOJTOVIČ D, LUHOVÁ L, PETŘIVALSKÝ M. Something smells bad to plant pathogens: Production of hydrogen sulfide in plants and its role in plant defence responses [J]. Journal of Advanced Research, 2021, 27: 199−209. doi: 10.1016/j.jare.2020.09.005 [6] 李顺, 景举伟, 严金平, 等. 气体信号分子H2S在植物中的研究进展 [J]. 植物生理学报, 2015, 51(5):579−585.LI S, JING J W, YAN J P, et al. Research progress in the gasotransmitter hydrogen sulfide in plants [J]. Plant Physiology Journal, 2015, 51(5): 579−585.(in Chinese) [7] 许智宏, 李家洋. 中国植物激素研究: 过去、现在和未来 [J]. 植物学通报, 2006, 41(5):433−442.XU Z H, LI J Y. Plant hormones research in China: Past, present and future [J]. Chinese Bulletin of Botany, 2006, 41(5): 433−442.(in Chinese) [8] CORREA-ARAGUNDE N, GRAZIANO M, CHEVALIER C, et al. Nitric oxide modulates the expression of cell cycle regulatory genes during lateral root formation in tomato [J]. Journal of Experimental Botany, 2006, 57(3): 581−588. doi: 10.1093/jxb/erj045 [9] SONG W J, XUE R, SONG Y, et al. Differential response of first-order lateral root elongation to low potassium involves nitric oxide in two tobacco cultivars [J]. Journal of Plant Growth Regulation, 2018, 37(1): 114−127. doi: 10.1007/s00344-017-9711-9 [10] LI H, GHOTO K, WEI M Y, et al. Unraveling hydrogen sulfide-promoted lateral root development and growth in mangrove plant Kandelia obovata: Insight into regulatory mechanism by TMT-based quantitative proteomic approaches [J]. Tree Physiology, 2021, 41(9): 1749−1766. doi: 10.1093/treephys/tpab025 [11] ZHANG X, ZHANG Y, ZHANG L Y, et al. Hydrogen sulphide improves iron homeostasis in wheat under iron-deficiency [J]. Journal of Plant Sciences, 2017, 5: 170. [12] WU X L, DU A Q, ZHANG S H, et al. Regulation of growth in peach roots by exogenous hydrogen sulfide based on RNA-Seq [J]. Plant Physiology and Biochemistry, 2021, 159: 179−192. doi: 10.1016/j.plaphy.2020.12.018 [13] 周超凡, 吴春涛, 李丹丹, 等. 外源H2S通过减轻低温光抑制增强黄瓜幼苗耐冷性 [J]. 植物生理学报, 2018, 54(3):411−420.ZHOU C F, WU C T, LI D D, et al. Hydrogen sulfide promotes chilling tolerance of cucumber seedlings by alleviating low-temperature photoinhibition [J]. Plant Physiology Journal, 2018, 54(3): 411−420.(in Chinese) [14] 周超凡, 吴帼秀, 李婷, 等. 外源H2S对低温下日光温室黄瓜光合作用及抗氧化系统的影响 [J]. 园艺学报, 2016, 43(3):462−472.ZHOU C F, WU G X, LI T, et al. Effect of exogenous hydrogen sulfide on photosynthesis and antioxidant system of cucumber leaves under low temperature in solar-greenhouse [J]. Acta Horticulturae Sinica, 2016, 43(3): 462−472.(in Chinese) [15] 裴雁曦, 贺烽, 解梦洁, 等. 气体信号分子H2S和NO对冷胁迫下大白菜幼苗光合作用的影响 [J]. 山西大学学报(自然科学版), 2017, 40(3):596−601.PEI Y X, HE F, XIE M J, et al. Effects of gasotransmitters NO and H2S on photosynthesis of Chinese cabbage seedlings under chilling stress [J]. Journal of Shanxi University (Natural Science Edition), 2017, 40(3): 596−601.(in Chinese) [16] DA SILVA C J, BATISTA FONTES E P, MODOLO L V. Salinity-induced accumulation of endogenous H2S and NO is associated with modulation of the antioxidant and redox defense systems in Nicotiana tabacum L. cv. Havana [J]. Plant Science, 2017, 256: 148−159. doi: 10.1016/j.plantsci.2016.12.011 [17] LI Z G. Analysis of some enzymes activities of hydrogen sulfide metabolism in plants [J]. Methods in Enzymology, 2015, 555: 253−269. [18] 曹刚, 张国斌, 郁继华, 等. 不同光质LED光源对黄瓜苗期生长及叶绿素荧光参数的影响 [J]. 中国农业科学, 2013, 46(6):1297−1304.CAO G, ZHANG G B, YU J H, et al. Effects of different LED light qualities on cucumber seedling growth and chlorophyll fluorescence parameters [J]. Scientia Agricultura Sinica, 2013, 46(6): 1297−1304.(in Chinese) [19] XUAN L J, LI J A, WANG X Y, et al. Crosstalk between hydrogen sulfide and other signal molecules regulates plant growth and development [J]. International Journal of Molecular Sciences, 2020, 21(13): 4593. doi: 10.3390/ijms21134593 [20] ARIF Y, HAYAT S, YUSUF M, et al. Hydrogen sulfide: A versatile gaseous molecule in plants [J]. Plant Physiology and Biochemistry, 2021, 158: 372−384. doi: 10.1016/j.plaphy.2020.11.045 [21] XUE Y F, ZHANG M, QI Z Q, et al. Cinnamaldehyde promotes root branching by regulating endogenous hydrogen sulfide [J]. Journal of the Science of Food and Agriculture, 2016, 96(3): 909−914. doi: 10.1002/jsfa.7164 [22] JIA H L, HU Y F, FAN T T, et al. Hydrogen sulfide modulates actin-dependent auxin transport via regulating ABPs results in changing of root development in Arabidopsis [J]. Scientific Reports, 2015, 5: 8251. doi: 10.1038/srep08251 -
点击查看大图
图(6) / 表(1)
计量
- 文章访问数: 459
- HTML全文浏览量: 173
- PDF下载量: 10
- 被引次数: 0
