Transcriptome Analysis on Effect of Glutamic and Aspartic Acids on Growth of Sparassis latifolia
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摘要:
目的 通过转录组测序分析添加不同氮源培养后广叶绣球菌基因差异表达情况，旨在探究氨基酸调节基质降解的分子机理，为其高效生产提供理论依据。 方法 以硫酸铵、鱼粉蛋白胨、天冬氨酸和谷氨酸为氮源，测定菌丝生长速度并利用转录组测序技术对不同氮源诱导下广叶绣球菌进行基因差异表达分析。 结果 天冬氨酸和谷氨酸相对于硫酸铵、鱼粉蛋白胨可显著促进菌丝生长（P＜0.01）；GO功能富集分析显示差异基因主要富集在氧化还原酶活性、铁离子跨膜转运蛋白活性和铁离子的还原和转运同化等；KEGG通路富集分析显示，富集度较高的通路主要有色氨酸代谢、花生四烯酸代谢和氮代谢等。差异基因中溶血素蛋白ostreolysin A6、GroES类似蛋白和6-甲基水杨酸脱羧酶等基因在天冬氨酸或谷氨酸诱导下表达量变化倍数较大（P＜0.01）。 结论 天冬氨酸和谷氨酸可能通过促进绣球菌氧化还原酶活性及铁离子代谢过程，提高其基质利用能力。研究结果为进一步研究广叶绣球菌木质纤维素降解机制及栽培生理提供理论依据。 Abstract:Objective Effects of differential expression genes (DEGs) of Sparassis latifolia cultured with varied nitrogen sources on substrate degradation were analyzed for efficient cultivation of the mushroom. Method Cultured on substrates of varied nitrogen sources, such as ammonium sulfate (As), fish peptone (Pep), aspartic acid (Asp), and glutamic acid (Glu), S. latifolia mycelium growth was observed. Transcriptomes of DEGs were determined. Result Of the various nitrogen sources, Asp and Glu significantly promoted the mycelial growth (P＜0.01). According to a GO enrichment analysis, the DEGs in the mushrooms were mainly related to the activities of oxidoreductase, iron ion transmembrane transport, and iron assimilation by reduction and transport. And the KEGG showed their involvement in the metabolisms of tryptophan, arachidonic acid, and nitrogen. The expressions of the ostreolysin A6, GroES-like protein, and 6-methylsalicylic acid decarboxylase genes were significantly altered by the presence of Asp and Glu (P＜0.01). Conclusion The use of Asp and Glu as a nitrogen source promoted the substrate utilization through the heightened oxidoreductase activity and iron metabolism in S. latifolia. Hence, the addition would facilitate an efficient mushroom cultivation. -
Key words:
- Sparassis latifolia /
- transcriptome /
- amino acid /
- glutamic acid /
- aspartic acid
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图 1 不同氮源对广叶绣球菌生长的影响
Figure 1. Effects of different nitrogen sources on growth of S. latifolia
图 2 差异表达基因统计图（A）和韦恩图（B）
Figure 2. Statistical histogram (A) and Venn diagram (B) of DEGs
图 4 差异显著基因热图聚类分析
热图上数字为基因表达量FPKM值，下同。
Figure 4. Heat map clustering on significantly differentiated genes
Datas are FPKM values. Same for below.
图 6 纤维素和半纤维素降解相关基因热图聚类分析
Figure 6. Heat map clustering on cellulose- and hemicellulose-degrading genes
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