Analysis of Genetypes Related to Important Agronomic Traits of Purple Waxy Special Rice Variety Ziliangyou 737
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摘要:
目的 解析紫糯特种稻紫两优737及其亲本的产量、品质相关性状的基因型构成，为该品种及其双亲在育种和生产上的进一步应用提供分子依据。 方法 应用10个重要基因的13个分子标记，分析紫两优737及其亲本紫392S、福恢737在产量、品质等相关基因座位上的等位基因变化。 结果 在7个基因座位上，紫两优737及其亲本均携带相同等位基因型。在穗实粒数主效基因Gn1a座位上均携带高产等位基因Ha-Gn1a；在粒重和粒长主效基因GS3座位上均携带长粒型等位基因MH-GS3；在种皮基因Pb座位上均携带紫色果皮等位基因型；在糯稻蜡质基因wx座位上均携带低直链淀粉含量等位基因型；此外在淀粉分支酶基因SBE1和SBE3以及极限糊精酶基因PUL基因座位上亦均携带相同等位基因型。在3个基因座位上，双亲携带不同等位基因型。在Hd3a座位上，紫392S、福恢737和紫两优737分别携带Hd3aNip、hd3aKasa和Hd3aNip/hd3aKasa；在Hd1座位上，分别携带Hd1jap、Hd1ind和Hd1jap/Hd1ind；在焦磷酸化酶大亚基基因AGPlar座位上，分别为Ⅰ型，Ⅱ型及Ⅰ/Ⅱ杂合型。 结论 紫两优737重要农艺性状的遗传解析为其具有高产、优质、适应性好及种皮颜色等特点提供了分子证据，并为相关有利等位基因的进一步利用提供参考。 Abstract:Objective This study was conducted to analyze the genotypes of yield and quality-related traits of the purple waxy special rice Ziliangyou 737 and its parents, and to provide molecular basis for the further application of this variety and its parents in breeding and production. Method Using 13 molecular markers of 10 important genes, the alleles of Ziliangyou 737 and its parents Ziliang392s and Fuhui 737 were analyzed in relation to yield and quality. Result In 7 gene loci, both Zizliangyou 737 and its parents carry the same allele genotype. The high-yield allele Ha-Gn1a was carried in the seat of Gn1a, the major gene of panicles number. The long grain allele MH-GS3 was carried in the GS3 locus of the major gene for grain weight and grain length. The alleles of purple pericarp were carried in the Pb locus of seed coat gene. All alleles with low amylose content in waxy gene wx locus were carried in glutinous rice. In addition, the same allelic genotype was carried in the starch branch enzyme genes SBE1 and SBE3 and the limit dextrin enzyme gene PUL gene locus. In three loci, parents carry different alleles. In the Hd3a seat, the Zi 392S, Fuhui 737 and Ziliangyou 737 carry Hd3aNip, hd3aKasa and Hd3aNip/hd3aKasa respectively. On the Hd1 seat, carry Hd1jap, Hd1ind and Hd1jap/Hd1ind respectively. In the large subunit gene AGPlar locus of pyrophosphorylase, there were type I, type II and hybrid type I/II, respectively. Conclusion The genetic analysis of important agronomic traits of Ziliangyou 737 provided molecular evidence for its high yield, high quality, good adaptability and seed coat color, and provided reference for further utilization of related favorable alleles -
Key words:
- Purple waxy hybrid rice /
- molecular markers /
- genotyping /
- high yield /
- high quality
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图 1 水稻产量因子Gn1a、 GS3基因相关标记对紫两优737及其亲本的检测结果
A：Gn1a-M1。M：20bp DNA Marker；1：明恢63；2：宜优673；3：紫392S；4：福恢737；5：紫两优737。B： GS3-PstⅠ，原始扩增产物（左）和酶切产物（右）。M：100 bp DNA Marker； 1：珍汕97，2：明恢63，3：紫392S，4：福恢737；5：紫两优737。
Figure 1. Detection of Ziliangyou 737 and its parents with markers for rice yield components Gn1a and GS3
A: Gn1a-M1. M: 20bp DNA Marker; 1: Minghui 63; 2: Yiyou 673; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. B: GS3-PstⅠ, original amplified products(left) and enzyme digestion products (right). M: 100 bp DNA Marker; 1: Zhengshan 97; 2: Minghui 63; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737.
图 2 水稻Hd3a、 Hd1基因相关标记对紫两优737及其亲本的检测结果
A： hd3afnp。M：100 bp DNA Marker；1：日本晴；2：Kasalath；3：紫392S；4：福恢737；5：紫两优737。B：Si9337。M：20 bp DNA Marker；1：日本晴；2：9311；3：紫392S；4：福恢737；5：紫两优737。
Figure 2. Detection of Ziliangyou 737 and its parents with markers for Hd3a and Hd1
A: hd3afnp. M: 100 bp DNA Marker; 1: Nipponbare; 2: Kasalath; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. B: Si9337. M: 20 bp DNA Marker; 1: Nipponbare; 2: 9311; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737.
图 3 糯稻蜡质基因相关标记对紫两优737及其亲本的检测结果
A：We 2-2。M：20 bp DNA Marker；1：荆糯6；2：9311；3：紫392S；4：福恢737；5：紫两优737。B：Wx-t1。M：20 bp DNA Marker；1：荆糯6；2：9311；3：紫392S；4：福恢737；5：紫两优737。C：484/W2R，原始扩增产物（左）和酶切产物（右）；M：100 bp DNA Marker；1：9311；2：明恢63；3：紫392S；4：福恢737；5：紫两优737。D：PCR-AccⅠ，原始扩增产物（左）和酶切产物（右）；M：100 bp DNA Marker；1：9311；2：明恢63；3：紫392S；4：福恢737；5：紫两优737。
Figure 3. Detection of Ziliangyou 737 and its parents with markers for wx
A: We 2-2. M: 20 bp DNA Marker; 1: Jingnuo 6; 2: 9311; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. B: Wx-t1. M: 20 bp DNA Marker; 1: Jingnuo 6; 2: 9311; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. C: 484/W2R, original amplified products (left) and enzyme digestion products (right); M: 100 bp DNA Marker; 1: 9311; 2: Minghui 63; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. D: PCR-AccⅠ, original amplified products (left) and enzyme digestion products (right); M: 100 bp DNA Marker; 1: 9311; 2: Minghui 63; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737.
图 4 水稻淀粉合成相关基因、紫色种皮基因Pb相关标记对紫两优737及其亲本的检测结果
A：Sbe1。M：100 bp DNA Marker。1：9311；2：明恢63；3：紫392S；4：福恢737；5：紫两优737。B：AGPlar M1。M：20 bp DNA Marker；1：珍汕97B；2：9311；3：紫392S；4：福恢737；5：紫两优737。C：PUL M2。M：20 bp DNA Marker；1：9311；2：宜优673；3：紫392S；4：福恢737；5：紫两优737。D：RS/SpeⅠ，原始扩增产物（左）和酶切产物（右）。M：100 bp DNA Marker；1：密阳23；2：宜优673；3：紫392S；4：福恢737；5：紫两优737。E：CAPSPb，原始扩增产物（左）和酶切产物（右）。M：100 bp DNA Marker；1：日本晴； 2：9311；3：紫392S；4：福恢737；5：紫两优737。
Figure 4. Detection of Ziliangyou 737 and its parents with markers for starch synthesis-related genes and Pb gene
A: Sbe1. M: 100 bp DNA Marker. 1: 9311; 2: Minghui 63; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. B: AGPlar M1. M: 20 bp DNA Marker; 1: Zhenshan 97B; 2: 9311; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. C: PUL M2. M: 20 bp DNA Marker; 1: 9311; 2: Yiyou 673; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. D: RS/SpeⅠ, original amplified products (left) and enzyme digestion products (right). M: 100 bp DNA Marker; 1: Miyang 23; 2: Yiyou 673; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737. E: CAPSPb, original amplified products (left) and enzyme digestion products (right). M: 100 bp DNA Marker; 1: Nipponbare; 2: 9311; 3: Zi 392S; 4: Fuhui 737; 5: Ziliangyou 737.
表 1 检测10个基因的相关标记信息
Table 1. Markers’ information for 10 genes
基因
Gene标记
Marker引物序列
Primer sequence (5′-3′)退火温度
Annealing temperature/℃等位基因参照
Allele referenceGn1a Gn1a-M1[21] CTCTTGCTTCATTATCAATC 55 明恢63 Minghui 63 AAACTACACAAGAATCTGCT GS3 GS3-PstⅠ[21]
（限制性内切酶 Restriction enzyme：PstⅠ）TATTTATTGGCTTGATTTCCTGTG 55 珍汕97 Zhenshan 97，明恢63 Minghui 63 GCTGGTTTTTTACTTTCATTTGCC Hd3a hd3afnp inner[22] AGCGGCAGGAGaGTCTACAA 62 日本晴 Nipponbare， Kasalath TCaGGATCATCGTTAGCTAGGG hd3afnp outer AAtCGAGGGGAGTATATTGCTAGT GCTaCATGAGAGACCTTAGCCTT Hd1 Si9337[23] AGATGTCCCTTCACTTCAGC 60 9311，日本晴 Nipponbare CGAAACGGCCCTTGATCC wx We 2-2[24] CACTACAAGACACACTTGCAC 55 荆糯6 Jingnuo 6, 9311 GTCATCTAGCCCACCACCTT Wx-t1[25] ATGTCGGCTCTCACCACG 55 荆糯6 Jingnuo 6, 9311 ACCGACCGCTGCTGCTTG 484/W2R[26]
（限制性内切酶 Restriction enzyme：AccⅠ）CTTTGTCTATCTCAAGACAC 55 9311，明恢63 Minghui 63 TTTCCAGCCCAACACCTTAC PCR- AccⅠ [27]
（限制性内切酶 Restriction enzyme：AccⅠ）GCTTCACTTCTCTGCTTGTG 55 日本晴Nipponbare, 明恢63 Minghui 63 ATGATTTAACGAGAGTTGAA Sbe1 Sbe1[28] GAGTTGAGTTGCGTCAGATC 57 9311，日本晴 Nipponbare AATGAGGTTGCTTGCTGCTG sbe3-rs RS/SpeⅠ[29]
（限制性内切酶 Restriction enzyme：SpeⅠ）ATGTGATGTGCTGGATTTGG 55 密阳 23 Miyang 23，宜优673 Yiyou 673 TGTGGTTTTCATACCGTTCTTA AGPlar AGPlar M1[30] CGTTCAGGTTCAGGCAATCA 58 珍汕97 Zhenshan 97, 9311 GGAAGGGTGGTGATGTGGAG PUL PUL M2[30] GACAACCGTCCGCTTTAGTTTC 58 9311, 宜优673 Yiyou 673 GCATTTGAGAGGGTTTGGATTC Pb CAPSPb [31]
（限制性内切酶 Restriction enzyme：BamHⅠ）AAATCAGTTGTCCCGTCCA 58 9311，日本晴 Nipponbare TTAGGGAGTTGGTGATGGG 
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表 2 紫两优737在云南、福建和安徽区域试验产量表现[1,20]
Table 2. Yield performance of Ziliangyou 737 in regional trails in Yunnan, Fujian and Anhui
试验类型
Type of test品种
Varieties平均产量
Average Yield/（kg·hm−2）比对照增减产
Ratio compared with CK/%云南区试 Reginal trial in Yunnan 紫两优737 Ziliangyou 737 7566.90 152.1.0 癸能紫米 Guinengzimi（CK） 3002.10 福建区试 Reginal trial in Fujian 紫两优737 Ziliangyou 737 7505.48 −3.82 宜优673 Yiyou 673（CK） 7804.07 安徽区试 Reginal trial in Anhui 紫两优737 Ziliangyou 737 8441.63 −0.16 Ⅱ优838（CK） 8456.55
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表 3 紫两优737在云南、福建区域试验稻米品质[1]
Table 3. Rice quality of Ziliangyou 737 in regional trails in Yunnan and Fujian
类别 Types 糙米率
Brown rice percentage/%精米率
Head rice percentage/%整精米率
Head rice percentage/%粒长
Grain length/mm长宽比 Ratio of grain length to width 直链淀粉 Amylase content/% 碱消值 Alkali value 胶稠度
Gel consistency/mm云南区试Regional trial in Yunnan 78.4 69.4 55.3 6.3 2.7 2.6 7.0 90 福建区试 Reginal trial of in Fujian 79.3 68.5 65.1 6.4 2.9 2.1 7.0 97
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[1] 黄庭旭, 郑建华, 游晴如, 等. 两系特种稻新品种紫两优737的选育与应用 [J]. 福建农业学报, 2020, 35(11):1171−1178. doi: 10.19303/j.issn.1008-0384.2020.11.001HUANG T X, ZHENG J H, YOU Q R, et al. Breeding and application of new two-line special rice variety ziliangyou 737 [J]. Fujian Journal of Agricultural Sciences, 2020, 35(11): 1171−1178.(in Chinese) doi: 10.19303/j.issn.1008-0384.2020.11.001 [2] 杨加珍, 曾亚文, 杜娟, 等. 紫黑米种质功能成分综合研究与利用 [J]. 生物技术进展, 2015, 5(1):47−53. doi: 10.3969/j.issn.2095-2341.2015.01.07YANG J Z, ZENG Y W, DU J, et al. Comprehensive utilization and research of functional components of purple black rice germplasm [J]. Current Biotechnology, 2015, 5(1): 47−53.(in Chinese) doi: 10.3969/j.issn.2095-2341.2015.01.07 [3] 常徽, 糜漫天, 凌文华. 黑米花色苷及联合化疗药物对不同肿瘤细胞增殖的影响 [J]. 第三军医大学学报, 2007, 29(20):1943−1946. doi: 10.3321/j.issn:1000-5404.2007.20.006CHANG H, MI M T, LING W H. Effects of anthocyanin-rich extract from black rice alone or combined with chemotherapeutic agents on proliferation of different cancer cells [J]. Acta Academiae Medicinae Militaris Tertiae, 2007, 29(20): 1943−1946.(in Chinese) doi: 10.3321/j.issn:1000-5404.2007.20.006 [4] CHANG H, YU B, YU X P, et al. Anticancer activities of an anthocyanin-rich extract from black rice against breast cancer cells in vitro and in vivo [J]. Nutrition and Cancer, 2010, 62(8): 1128−1136. doi: 10.1080/01635581.2010.494821 [5] 路宏朝, 王杨科, 李丽霞, 等. 黑米花青苷复方胶囊对实验性肝损伤的保护作用 [J]. 食品科学, 2013, 34(3):261−263.LU H Z, WANG Y K, LI L X, et al. Protective effect of black rice anthocyanin compound capsule against experimental hepatic injury [J]. Food Science, 2013, 34(3): 261−263.(in Chinese) [6] 贾艳梅, 郝旭, 赵龙. 黑米花青素对丝绸的染色及其抗紫外线性能 [J]. 现代纺织技术, 2022, 30(3):174−178,185. doi: 10.19398/j.att.202105035JIA Y M, HAO X, ZHAO L. Dyeing behavior and anti-ultraviolet property of silk fabric dyed with black rice anthocyanin [J]. Advanced Textile Technology, 2022, 30(3): 174−178,185.(in Chinese) doi: 10.19398/j.att.202105035 [7] 赵腾芳. 黑糯稻品种资源的考察 [J]. 云南农业科技, 1985(1):29−31,27.ZHAO T F. Investigation on black glutinous rice variety resources [J]. Yunnan Agricultural Science and Technology, 1985(1): 29−31,27.(in Chinese) [8] 韩龙植, 南钟浩, 全东兴, 等. 特种稻种质创新与营养特性评价 [J]. 植物遗传资源学报, 2003, 4(3):207−213. doi: 10.3969/j.issn.1672-1810.2003.03.006HAN L Z, NAN Z H, QUAN D X, et al. Prebreeding and nutritive characteristic evaluation of special rice [J]. Journal of Plant Genetic Resources, 2003, 4(3): 207−213.(in Chinese) doi: 10.3969/j.issn.1672-1810.2003.03.006 [9] 王延春, 夏志辉, 刘迪, 等. 特种稻新品种中科黑糯1号的选育 [J]. 种子, 2013, 32(10):105−106. doi: 10.3969/j.issn.1001-4705.2013.10.032WANG Y C, XIA Z H, LIU D, et al. Breeding of a new special rice variety zhongke Heinuo No. 1 [J]. Seed, 2013, 32(10): 105−106.(in Chinese) doi: 10.3969/j.issn.1001-4705.2013.10.032 [10] 黎用朝, 闵军, 刘三雄, 等. 特种稻新品种晚籼紫宝的选育与应用 [J]. 中国稻米, 2015, 21(3):75−76. doi: 10.3969/j.issn.1006-8082.2015.03.020LI Y C, MIN J, LIU S X, et al. Breeding and application of new indica rice variety wanxianzibao [J]. China Rice, 2015, 21(3): 75−76.(in Chinese) doi: 10.3969/j.issn.1006-8082.2015.03.020 [11] 闵军, 黎用朝, 刘三雄, 等. 紫米型新品种丽人紫的选育 [J]. 中国稻米, 2015, 21(6):107−108. doi: 10.3969/j.issn.1006-8082.2015.06.027MIN J, LI Y C, LIU S X, et al. Breeding of new indica rice variety lirenzi with brown rice [J]. China Rice, 2015, 21(6): 107−108.(in Chinese) doi: 10.3969/j.issn.1006-8082.2015.06.027 [12] 台德卫, 蒋光月, 姚自鸣, 等. 功能性水稻新品种品黑一号的选育及营养安全研究初报 [J]. 安徽农学通报, 2016, 22(5):20−21,115. doi: 10.3969/j.issn.1007-7731.2016.05.009TAI D W, JIANG G Y, YAO Z M, et al. Preliminary report of the breeding and nutritional evaluation of a newly bred black rice variety pinhei 1 [J]. Anhui Agricultural Science Bulletin, 2016, 22(5): 20−21,115.(in Chinese) doi: 10.3969/j.issn.1007-7731.2016.05.009 [13] 唐清杰, 王惠艰, 严小微. 特种稻品种海丰黑糯2号的选育及栽培技术要点 [J]. 中国稻米, 2018, 24(1):111−112. doi: 10.3969/j.issn.1006-8082.2018.01.029TANG Q J, WANG H J, YAN X W. Breeding and application of new special rice Haifengheinuo 2 [J]. China Rice, 2018, 24(1): 111−112.(in Chinese) doi: 10.3969/j.issn.1006-8082.2018.01.029 [14] 黄春毓, 张志英, 李伟荣, 等. 紫色特种稻坤两优1号的选育及应用 [J]. 种子, 2019, 38(12):128−131,141. doi: 10.16590/j.cnki.1001-4705.2019.12.128HUANG C Y, ZHANG Z Y, LI W R, et al. Breeding and application of purple special rice kunliangyou No. 1 [J]. Seed, 2019, 38(12): 128−131,141.(in Chinese) doi: 10.16590/j.cnki.1001-4705.2019.12.128 [15] 张志英, 陈春莲, 李伟荣, 等. 紫黑色特种两系杂交水稻新组合坤两优22 [J]. 杂交水稻, 2020, 35(4):96−98. doi: 10.16267/j.cnki.1005-3956.20190902.220ZHANG Z Y, CHEN C L, LI W R, et al. Kunliangyou 22, a new purple-black special two-line hybrid rice combination [J]. Hybrid Rice, 2020, 35(4): 96−98.(in Chinese) doi: 10.16267/j.cnki.1005-3956.20190902.220 [16] 唐清杰, 韩义胜, 严小微. 多抗基因聚合的特种稻海丰黑糯2号抗病虫鉴定和分子标记检测 [J]. 分子植物育种, 2018, 16(2):466−471. doi: 10.13271/j.mpb.016.000466TANG Q J, HAN Y S, YAN X W. Identification of resistance to diseases and pests and molecular market detection of a special rice variety Haifengheinuo 2 with multiple gene polymerization [J]. Molecular Plant Breeding, 2018, 16(2): 466−471.(in Chinese) doi: 10.13271/j.mpb.016.000466 [17] 王军, 杨金欢, 杨杰, 等. 优质紫香糯龙晴4号的紫色和香味的基因型分析 [J]. 分子植物育种, 2011, 9(6):688−691. doi: 10.3969/mpb.009.000688WANG J, YANG J H, YANG J, et al. Genotypic identification of the fragrance and purple in the high quality sticky rice Longqing4 [J]. Molecular Plant Breeding, 2011, 9(6): 688−691.(in Chinese) doi: 10.3969/mpb.009.000688 [18] 许峰, 宋学堂, 陈露, 等. 香血稻515的选育及栽培技术 [J]. 中国稻米, 2021, 27(1):109−110,113.XU F, SONG X T, CHEN L, et al. Breeding and cultivation techniques of Xiangxuedao 515 [J]. China Rice, 2021, 27(1): 109−110,113.(in Chinese) [19] 朱丽, 钱前. 突破复杂性状多基因转化技术壁垒, 首创胚乳花青素高积累的水稻新种质 [J]. 植物学报, 2017, 52(5):539−542. doi: 10.11983/CBB17126ZHU L, QIAN Q. Development of “purple endosperm rice” by engineering anthocyanin biosynthesis in endosperm: Significant breakthrough in transgene stacking system, new progress in rice biofortification [J]. Chinese Bulletin of Botany, 2017, 52(5): 539−542.(in Chinese) doi: 10.11983/CBB17126 [20] 郑建华, 王洪飞, 周鹏, 等. 籼型紫黑糯稻光温敏核不育系紫392S选育与应用 [J]. 杂交水稻, 2021, 36(6):13−17. doi: 10.16267/j.cnki.1005-3956.20200928.306ZHENG J H, WANG H F, ZHOU P, et al. Breeding and application of PTGMS line zi 392S of indica-type glutinous rice with purple-black grains [J]. Hybrid Rice, 2021, 36(6): 13−17.(in Chinese) doi: 10.16267/j.cnki.1005-3956.20200928.306 [21] YAN C J, YAN S, YANG Y C, et al. Development of gene-tagged markers for quantitative trait loci underlying rice yield components [J]. Euphytica, 2009, 169(2): 215−226. doi: 10.1007/s10681-009-9937-0 [22] 常远, 吴苏亭, 杨毅荣, 等. 4引物分子标记鉴定水稻高光效基因型hd3aKasa [J]. 杂交水稻, 2020, 35(4):75−80.CHANG Y, WU S T, YANG Y R, et al. Identification of the genotype of hd3aKasa with a high photosynthetic efficiency in rice by a Tetra-primer molecular marker [J]. Hybrid Rice, 2020, 35(4): 75−80.(in Chinese) [23] 陈俊宇, 王凯, 龚俊义, 等. RFT1与Hd1所在区间对水稻抽穗期、株高和千粒重的作用 [J]. 中国水稻科学, 2013, 27(2):117−121. doi: 10.3969/j.issn.1001-7216.2013.02.002CHEN J Y, WANG K, GONG J Y, et al. Effects of the RFT1 region and Hd1 region on heading date, plant height and thousand-grain weight in rice(Oryza sativa) [J]. Chinese Journal of Rice Science, 2013, 27(2): 117−121.(in Chinese) doi: 10.3969/j.issn.1001-7216.2013.02.002 [24] 孙华钦, 田洁, 郑家奎, 等. 糯稻和非糯稻蜡质基因的新STS分子标记 [J]. 应用与环境生物学报, 2006, 12(4):461−463. doi: 10.3321/j.issn:1006-687X.2006.04.004SUN H Q, TIAN J, ZHENG J K, et al. New STS molecular markers for waxy gene of glutinous and nonglutinous rices [J]. Chinese Journal of Applied & Environmental Biology, 2006, 12(4): 461−463.(in Chinese) doi: 10.3321/j.issn:1006-687X.2006.04.004 [25] 谢会兰. 水稻淀粉合成相关基因分子标记的建立及其遗传网络初步探析[D]. 扬州: 扬州大学, 2007XIE H L. Establishment of molecular markers related to rice starch synthesis and preliminary analysis of its genetic network[D]. Yangzhou: Yangzhou University, 2007. (in Chinese) [26] 侯立恒, 夏明元, 戚华雄, 等. 利用Wx基因分子标记辅助选择技术培育中等直链淀粉含量的水稻恢复系 [J]. 中国农学通报, 2009, 25(14):32−36.HOU L H, XIA M Y, QI H X, et al. Developing restorer lines with intermediate amylose content by molecular marker-assisted selection in rice [J]. Chinese Agricultural Science Bulletin, 2009, 25(14): 32−36.(in Chinese) [27] 蔡秀玲, 刘巧泉, 汤述翥, 等. 用于筛选直链淀粉含量为中等的籼稻品种的分子标记 [J]. 植物生理与分子生物学学报, 2002, 28(2):137−144.CAI X L, LIU Q Q, TANG S Z, et al. Development of a molecular marker for screening the rice cultivars with intermediate amylose content in Oryza sativa subsp. indica [J]. Acta Photophysiologica Sinica, 2002, 28(2): 137−144.(in Chinese) [28] 严长杰, 田舜, 张正球, 等. 水稻栽培品种淀粉合成相关基因来源及其对品质的影响 [J]. 中国农业科学, 2006, 39(5):865−871. doi: 10.3321/j.issn:0578-1752.2006.05.001YAN C J, TIAN S, ZHANG Z Q, et al. The source of genes related to rice grain starch synthesis among cultivated varieties and its contribution to quality [J]. Scientia Agricultura Sinica, 2006, 39(5): 865−871.(in Chinese) doi: 10.3321/j.issn:0578-1752.2006.05.001 [29] 杨瑞芳, 白建江, 方军, 等. 分子标记辅助选择选育高抗性淀粉水稻新品种 [J]. 核农学报, 2015, 29(12):2259−2267. doi: 10.11869/j.issn.100-8551.2015.12.2259YANG R F, BAI J J, FANG J, et al. Establishment of marker-assisted selection system for breeding rice varieties with high resistant starch content [J]. Journal of Nuclear Agricultural Sciences, 2015, 29(12): 2259−2267.(in Chinese) doi: 10.11869/j.issn.100-8551.2015.12.2259 [30] 田志喜, 严长杰, 钱前, 等. 水稻淀粉合成相关基因分子标记的建立 [J]. 科学通报, 2010, 55(26):2591−2601. doi: 10.1360/csb2010-55-26-2591TIAN Z X, YAN C J, QIAN Q, et al. Establishment of molecular markers for genes related to starch synthesis in rice [J]. Chinese Science Bulletin, 2010, 55(26): 2591−2601.(in Chinese) doi: 10.1360/csb2010-55-26-2591 [31] 王芳权, 杨杰, 范方军, 等. 水稻紫色果皮的延迟遗传及Pb基因功能标记开发 [J]. 中国水稻科学, 2014, 28(6):605−611. doi: 10.3969/j.issn.1001-7216.2014.06.006WANG F Q, YANG J, FAN F J, et al. Delayed inheritance of purple pericarp in rice and development of functional marker for Pb gene [J]. Chinese Journal of Rice Science, 2014, 28(6): 605−611.(in Chinese) doi: 10.3969/j.issn.1001-7216.2014.06.006 [32] 连玲, 潘丽燕, 朱永生, 等. 杂交水稻骨干亲本Wx基因第一内含子+1位碱基多态性分析 [J]. 福建农业学报, 2019, 34(12):1355−1363.LIAN L, PAN L Y, ZHU Y S, et al. Polymorphisms on first base of wx gene intron 1 in parents of hybrid rice [J]. Fujian Journal of Agricultural Sciences, 2019, 34(12): 1355−1363.(in Chinese) [33] 康翠芳, 向珣朝, 龙小林, 等. 水稻淀粉合成相关基因SSⅠ、SSⅢ-1和PUL对稻米品质的影响 [J]. 农业生物技术学报, 2015, 23(3):311−319. doi: 10.3969/j.issn.1674-7968.2015.03.004KANG C F, XIANG X C, LONG X L, et al. Effects of the starch-synthesizing genes SSⅠ, SSⅢ-1 and PUL on rice(Oryza sativa L. ) quality [J]. Journal of Agricultural Biotechnology, 2015, 23(3): 311−319.(in Chinese) doi: 10.3969/j.issn.1674-7968.2015.03.004 [34] 王洪飞, 陈建云, 郑建华, 等. 两系特种稻紫两优737在云南高原种植表现及丰产栽培技术 [J]. 福建稻麦科技, 2020, 38(2):21−24. doi: 10.3969/j.issn.1008-9799.2020.02.010WANG H F, CHEN J Y, ZHENG J H, et al. Planting performance and high-yielding cultivation techniques of two-line special rice ziliangyou 737 in Yunnan Plateau [J]. Fujian Science and Technology of Rice and Wheat, 2020, 38(2): 21−24.(in Chinese) doi: 10.3969/j.issn.1008-9799.2020.02.010 [35] 张启发. 保障粮食安全, 促进营养健康: 黑米主食化未来可期 [J]. 华中农业大学学报, 2021, 40(3):1−2.ZHANG Q F. Ensuring food security and promoting nutrition and health: Making black rice staple food for the future [J]. Journal of Huazhong Agricultural University, 2021, 40(3): 1−2.(in Chinese) [36] 韩月澎, 徐明良, 严长杰, 等. 水稻糯性突变对淀粉理化特性的影响 [J]. 中国水稻科学, 2004, 18(2):125−129. doi: 10.3321/j.issn:1001-7216.2004.02.008HAN Y P, XU M L, YAN C J, et al. Effect of wx mutation on starch physical-chemical properties in rice [J]. Chinese Journal of Rice Science, 2004, 18(2): 125−129.(in Chinese) doi: 10.3321/j.issn:1001-7216.2004.02.008 [37] 孙业盈, 吕彦, 董春林, 等. 水稻Wx基因与稻米AC、GC和GT的遗传关系 [J]. 作物学报, 2005, 31(5):535−539. doi: 10.3321/j.issn:0496-3490.2005.05.001SUN Y Y, LYU Y, DONG C L, et al. Genetic relationship among wx gene, AC, GC and GT of rice [J]. Acta Agronomica Sinica, 2005, 31(5): 535−539.(in Chinese) doi: 10.3321/j.issn:0496-3490.2005.05.001 [38] HU B, WANG W, OU S J, et al. Variation in NRT1.1B contributes to nitrate-use divergence between rice subspecies [J]. Nature Genetics, 2015, 47(7): 834−838. doi: 10.1038/ng.3337 -
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