Regulating Effect of Exogenous Melatonin on Aluminum Toxicity in Solanum lycopersicum L.
-
摘要:
目的 研究褪黑素对铝胁迫下番茄生理上的调控作用，为缓解土壤酸铝化对番茄生长造成的不利影响提供理论依据。 方法 以耐铝品种千禧番茄和铝敏感品种普罗旺斯番茄为供试植物，设置空白组、单铝组、褪黑素（MT）处理组和铝+褪黑素共同施加组，测定各处理组生长周期（10 d、20 d、30 d）过程中番茄叶片抗氧化酶[超氧化物歧化酶（SOD）、过氧化物酶（POD）和过氧化氢酶（CAT）]活性、丙二醛（MDA）、脯氨酸（Pro）、抗坏血酸（AsA）、谷胱甘肽（GSH）含量、叶绿素含量和叶绿素荧光参数，检测根部铝富集含量和根系活力，并对番茄的根、茎、叶进行DNA损伤检测。 结果 铝逆境下两品种番茄生长发育均受到严重抑制，各器官DNA均受损严重，且其根系DNA损伤最为严重。施用褪黑素能够明显缓解番茄的铝毒症状，两品种番茄株高、根长及叶面积等主要生长指标均有所增长；抗氧化酶活性得到显著提高，且随实验周期延长呈现先上升后下降的趋势；GSH和AsA含量最大增幅达91.14%、13.52%；MDA含量降幅最大可达38.39%，脯氨酸含量增幅可达144.81%，调节番茄体内渗透平衡；叶绿素含量明显升高，各项荧光参数得到改善，恢复其光合能力；千禧番茄和普罗旺斯番茄根系活力增幅分别为5.19%和43.03%，两者的根、茎、叶铝富集能力均降低。同时，施加褪黑素也能够有效减缓番茄各器官DNA拖尾现象，修复细胞DNA损伤。 结论 施加外源褪黑素能有效激活铝胁迫下番茄的各项生理响应，促进其生长发育，不仅能提高抗氧化酶系统活性，增强光合能力与根系活力，还可以减少植株体内铝含量，降低细胞DNA受损程度，进一步提升其抗铝能力，为探索MT对逆境下植物的调控作用提供新思路。 Abstract:Objective Regulating effect of melatonin application on the physiology of a tomato plant under aluminum stress was studied. Method Al-tolerant Millennial tomato and Al-sensitive Provence tomato plants were grown in blank control, Al-added, melatonin-added (MT), or Al-and-melatonin-added (AMT) potting soils. Activities of superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), contents of malondialdehyde (MDA), proline (Pro), ascorbic acid (AsA), glutathione (GSH), and chlorophyll as well as chlorophyll fluorescence parameters, Al-accumulation, and root activity in the plants were monitored. DNA damage occurred to the roots, stems, and leaves of the plants during the experimentation were recorded. Statistical analysis was performed on all collected data using the one-way ANOVA and Duncan test. Result Under Al-stress, the growth and development of the tomato plants of either variety were severely inhibited, and the DNA in the organs, especially the roots, seriously damaged. Whereas the presence of melatonin in the soil (MT and/or AMT ) significantly alleviated the symptoms of Al-toxicity on the plants. For instance, with the addition, the main growth indicators, such as plant height, root length, and leaf area, were improved; the activity of antioxidant enzymes significantly heightened with an increasing trend initially and followed by a decline as treatment duration prolonged; the contents of GSH and AsA raised by 91.14% and 13.52% respectively; the reduction on MDA maximized at 38.39%; proline, which regulates the osmotic balance in tomato, increased to 144.81% and chlorophyll significantly; the fluorescence parameters improved; the photosynthetic capacity much restored; the root activity of Millennial tomato plant increased by 5.19%, and that of Provence tomato by 43.03%; the Al-accumulation in the roots, stems, and leaves significantly reduced; the DNA tailing in organs slowed; and the repairs on damaged DNA in cells observed. Conclusion Applying exogenous melatonin in soil effectively activated various physiological responses, raised the activity of antioxidant enzymes, enhanced the plant photosynthesis, reduced damage on cellular DNA, and elevated the resistance to Al-toxicity of the tomato plants under the heavy metal stress. -
Key words:
- Melatonin /
- Solanum lycopersicum /
- aluminum stress /
- physiological response /
- DNA damage
-
图 1 各处理下番茄根系活力的变化
不同小写字母表示同一品种不同处理间差异显著（P＜0.05）。
Figure 1. Changes on tomato root activity under treatments
Datas with different lowercase letters indicate significant differences between treatments on same tomato variety at P＜0.05.
图 2 外源MT对铝胁迫下番茄DNA损伤的影响
Figure 2. Effect of MT on DNA damage on tomato plants under Al-stress
表 1 外源MT对铝胁迫下番茄生长特性的影响
Table 1. Effect of MT on growth of tomato plants under Al-stress
品种
Varieties处理组
Treatments株高
Plant height/cm叶面积
leaf area/cm2根长
Root length/cm千禧 Qianxi CK 64.25±1.35 b 49.16±2.60 a 9.75±0.76 a Al 59.32±1.44 c 33.09±2.24 b 4.33±2.35 b MT 69.16±2.07 a 60.76±3.14 a 11.59±1.35 a Al+MT 65.92±3.12 b 55.40±1.47 a 10.47±1.32 a 普罗旺斯 Puluowangsi CK 59.37±1.21 b 37.95±1.24 a 12.64±1.84 a Al 51.31±2.74 c 25.88±3.27 b 8.24±2.46 b MT 62.81±2.68 a 41.84±1.55 a 16.05±1.27 a Al+MT 60.44±1.67 ab 39.62±2.47 a 14.18±0.55 a 表内数据为平均值±标准误差，同项同列数据后相同字母表示无显著差异（P > 0.05），下同。
Datas are mean ± standard error; those with same letter on same line indicate no significant difference ( P>0.05). Same for below. 
下载: 导出CSV
表 2 外源MT对铝胁迫下番茄抗氧化酶的影响
Table 2. Effects of MT on antioxidant enzymes of tomato plants under Al-stress
测量指标
Measured indicators处理
Treatment千禧 Qianxi 普罗旺斯 Puluowangsi 10 d 20 d 30 d 10 d 20 d 30 d SOD/(U·g−1) CK 3902.26±334.93 b 950.64±36.19 c 675.79±15.47 a 4457.17±75.413 a 906.62±17.75 d 583.64±12.05 b AI 6536.58±237.21 a 1288.10±16.24 ab 529.74±5.01 b 5609.25±138.00 a 1139.10±25.28 b 472.19±7.09 c MT 4421.31±299.02 b 1142.93±41.86 bc 694.85±33.19 a 4712.67±180.63 a 1041.19±24.62 c 595.65±29.14 b AI+MT 8008.05±193.70 a 1407.24±34.62 a 629.48±11.85 a 6600.04±281.52 a 1314.23±32.45 a 813.36±28.87 a POD/
(△OD470·min−1·g−1)CK 58.25±2.72 a 75.33±2.21 b 163.72±7.11 c 110.56±12.98 bc 113.50±8.86 b 118.62±10.85 c AI 55.01±14.66 c 95.76±11.25 b 97.96±13.18 d 42.86±8.97 bc 138.95±9.53 ab 95.42±4.70 d MT 64.88±7.39 ab 80.43±11.95 b 192.96±3.54 b 93.16±6.10 bc 124.86±8.80 ab 171.23±6.18 b AI+MT 73.31±3.57 a 184.41±26.62 a 238.29±9.09 a 114.05±7.30 ab 152.73±5.39 a 193.36±10.77 a CAT/
(μmol·min−1·g−1)CK 5.89±1.53 c 18.36±3.04 b 58.60±1.42 b 9.06±0.99 d 18.50±3.72 d 73.77±1.31 b AI 24.54±3.74 b 32.18±2.51 b 53.00±0.98 c 69.52±2.06 c 60.60±0.92 c 52.53±1.32 c MT 12.03±3.40 c 21.47±4.46 b 29.31±1.43 b 63.31±1.43 b 73.77±0.49 b 83.63±1.89 a AI+MT 61.12±1.96 a 77.17±1.21 a 74.67±1.39 a 74.67±1.39 a 89.77±2.03 a 82.17±1.88 a
下载: 导出CSV
表 3 外源MT对铝胁迫下番茄内源性抗氧化物的影响
Table 3. Effect of MT on antioxidants in tomato plants under Al-stress
测量指标
Measured indicators处理
Treatment千禧 Qianxi 普罗旺斯 Puluowangsi 10 d 20 d 30 d 10 d 20 d 30 d GSH含量
GSH content/(μmol·g−1)CK 0.44±0.01 d 1.07±0.043 a 1.06±0.05 c 0.66±0.01 d 1.11±0.02 b 1.10±0.02 b AI 1.14±0.01 b 0.86±0.03 a 0.89±0.03 d 1.09±0.02 b 1.06±0.02 c 0.66±0.02 c MT 0.61±0.01 c 1.20±0.02 a 1.69±0.02 a 0.71±0.01 c 1.26±0.01 b 1.30±0.04 a AI+MT 1.20±0.02 a 1.65±0.09 a 1.49±0.05 b 1.13±0.02 a 1.57±0.01 a 1.39±0.02 a AsA含量
AsA content/(mg·g−1)CK 0.03±0.01 d 0.18±0.02 c 0.22±0.03 b 0.14±0.01 d 0.12±0.01 d 0.20±0.02 b AI 0.36±0.01 b 0.39±0.01 b 0.11±0.02 c 0.30±0.05 b 0.28±0.05 b 0.13±0.02 c MT 0.36±0.01 c 0.41±0.02 ab 0.37±0.02 a 0.16±0.03 c 0.16±0.04 c 0.22±0.02 b AI+MT 0.40±0.02 a 0.44±0.02 a 0.36±0.03 a 0.31±0.03 a 0.29±0.03 a 0.26±0.04 a
下载: 导出CSV
表 4 外源MT对铝胁迫下番茄脯氨酸和MDA含量的影响
Table 4. Effects of MT on Pro and MDA contents in tomato plants under Al-stress
测量指标
Measured indicators处理
Treatment千禧 Qianxi 普罗旺斯 Puluowangsi 10 d 20 d 30 d 10 d 20 d 30 d MDA含量
MDA content/(nmol·g−1)CK 8.08±0.71 b 6.98±0.40 b 6.19±0.49 c 6.41±0.24 b 6.27±0.72 ab 7.77±0.65 c AI 9.47±0.65 a 8.03±0.47 a 8.01±0.48 a 7.49±0.73 a 7.11±0.70 a 10.17±0.93 a MT 3.43±0.95 ab 4.12±1.15 b 5.83±0.68 c 2.36±0.46 c 5.49±0.52 b 6.95±0.80 c AI+MT 8.75±0.63 c 6.76±0.65 c 6.82±0.62 b 7.20±0.22 a 6.52±0.85 ab 8.72±0.38 b 脯氨酸含量
Proline content/(mg·g−1)CK 4.94±1.33 c 6.39±1.16 c 16.47±1.90 d 2.24±0.97 c 5.10±1.91 b 13.12±1.20 b AI 5.54±1.63 c 9.63±1.43 b 20.23±1.20 c 3.77±0.99 c 8.26±1.82 b 14.43±2.09 b MT 9.64±1.15 b 14.39±1.23 a 34.06±1.06 b 6.48±1.54 b 12.34±2.00 a 25.86±2.03 a AI+MT 12.64±1.20 a 15.34±2.21 a 45.53±2.08 a 13.25±2.86 a 13.89±3.00 a 28.16±3.06 a
下载: 导出CSV
表 5 外源MT对铝胁迫下番茄光合特性的影响
Table 5. Effects of MT on photosynthetic characteristics of tomato plants under Al-stress
测量指标
Measured indicators处理
Treatment千禧 Qianxi 普罗旺斯 Puluowangsi 10 d 20 d 30 d 10 d 20 d 30 d SPAD值
SPAD valueCK 28.17±0.37 a 28.60±0.12 b 35.30±0.32 b 31.37±0.62 b 35.87±0.47 b 35.00±0.38 b AI 24.97±0.32 b 25.67±0.26 c 27.30±0.32 c 28.93±0.77 b 29.67±0.23 c 30.27±0.43 c MT 28.23±0.38 a 30.43±0.32 a 37.03±0.08 a 36.10±0.98 a 40.37±0.27 a 38.73±0.68 a AI+MT 27.03±0.46 a 28.93±0.20 b 34.53±0.55 b 31.40±1.36 b 36.27±0.45 b 34.40±0.10 b 初始荧光
FoCK 0.10±0.03 a 0.11±0.04 b 0.11±0.02 c 0.10±0.03 b 0.10±0.07 d 0.11±0.06 c AI 0.09±0.01 a 0.13±0.04 a 0.13±0.07 a 0.12±0.04 a 0.16±0.01 a 0.18±0.05 a MT 0.06±0.03 c 0.07±0.06 d 0.09±0.05 d 0.09±0.06 c 0.11±0.07 c 0.11±0.04 c AI+MT 0.08±0.02 b 0.09±0.05 c 0.11±0.05 b 0.11±0.03 b 0.12±0.04 b 0.14±0.03 b PSII 最大光化学量子产量
Fv /F mCK 0.92±0.03 b 0.93±0.02 a 0.93±0.04 a 0.82±0.03 d 0.81±0.02 b 0.82±0.03 b AI 0.84±0.02 d 0.73±0.03 d 0.69±0.02 c 0.72±0.03 b 0.65±0.03 d 0.61±0.02 d MT 1.04±0.01 a 1.00±0.01 a 0.94±0.02 a 0.94±0.02 a 0.93±0.03 a 0.95±0.03 a AI+MT 0.88±0.01 c 0.78±0.03 c 0.75±0.01 b 0.79±0.01 c 0.71±0.01 c 0.70±0.02 c
下载: 导出CSV
表 6 外源MT对铝胁迫下番茄铝富集效能的影响
Table 6. Effect of MT on Al-accumulation of tomato plants under Al-stress
品种 处理 Al含量 Al content/(mg·kg-1) Al富集系数 Al enrichment factor 转运系数
Coefficient of transshipment根
Root茎
Stem叶
Leaf土壤
Soil根
Root茎
Stem叶
Leaf千禧 Qianxi CK 0.13±0.00 c 0.10±0.00 b 0.07±0.01 c 0.83±0.03 bc 0.16±0.01 b 0.12±0.01 b 0.08±0.01 ab 1.29±0.005 a Al 0.22±0.02 a 0.14±0.00 a 0.10±0.02 ab 1.02±0.01 a 0.21±0.02 a 0.14±0.01 a 0.10±0.01 a 1.16±0.003 b MT 0.12±0.02 c 0.07±0.01 b 0.06±0.01 b 0.86±0.02 c 0.15±0.02 b 0.09±0.01 c 0.07±0.02 b 1.09±0.002 c Al+MT 0.18±0.01 b 0.13±0.01 a 0.09±0.01 ab 1.11±0.03 ab 0.17±0.01 b 0.12±0.02 b 0.08±0.01 b 1.20±0.002 b 普罗旺斯 Puluo wangsi CK 0.11±0.01 b 0.09±0.01 b 0.05±0.01 b 0.82±0.06 c 0.13±0.01 a 0.11±0.02 ab 0.06±0.01 b 1.37±0.003 b Al 0.15±0.01 a 0.13±0.01 a 0.09±0.001 a 1.02±0.01 ab 0.15±0.03 a 0.13±0.01 a 0.09±0.01 a 1.47±0.004 a MT 0.10±0.01 b 0.08±0.01 b 0.05±0.006 b 0.85±0.03 bc 0.12±0.01 a 0.10±0.01 b 0.06±0.01 b 1.38±0.002 b Al+MT 0.14±0.01 a 0.12±0.01 a 0.08±0.002 a 1.10±0.01 a 0.14±0.01 a 0.11±0.01 b 0.07±0.01 ab 1.32±0.001 c
下载: 导出CSV
-
[1] KIMMEL K, FUREY G N, HOBBIE S E, et al. Diversity-dependent soil acidification under nitrogen enrichment constrains biomass productivity [J]. Global Change Biology, 2020, 26(11): 6594−6603. doi: 10.1111/gcb.15329 [2] RIAZ M, YAN L, WU X W, et al. Boron reduces aluminum-induced growth inhibition, oxidative damage and alterations in the cell wall components in the roots of trifoliate orange [J]. Ecotoxicology and Environmental Safety, 2018, 153: 107−115. doi: 10.1016/j.ecoenv.2018.02.002 [3] YANG Y, MA L, ZENG H, et al. iTRAQ-based proteomics screen for potential regulators of wheat (Triticum aestivum L. ) root cell wall component response to Al stress [J]. Gene, 2018, 675: 301−311. doi: 10.1016/j.gene.2018.07.008 [4] 黄凯, 张红宇, 张菡倩, 等. 植物应答铝毒的分子机制研究进展 [J]. 生物技术通报, 2021, 37(3):125−135.HUANG K, ZHANG H Y, ZHANG H Q, et al. Research progress on the molecular mechanism of plants response to aluminum toxicity [J]. Biotechnology Bulletin, 2021, 37(3): 125−135.(in Chinese) [5] GATSIOS A, NTATSI G, YFANTOPOULOS D, et al. Effects of different organic soil amendments on nitrogen nutrition and yield of organic greenhouse tomato crop [J]. Nitrogen, 2021, 2(3): 347−358. doi: 10.3390/nitrogen2030024 [6] YANG Q A, PENG Z P, MA W N, et al. Melatonin functions in priming of stomatal immunity in Panax notoginseng and Arabidopsis thaliana [J]. Plant Physiology, 2021, 187(4): 2837−2851. doi: 10.1093/plphys/kiab419 [7] ZAHEDI S M, HOSSEINI M S, FAHADI H N, et al. Exogenous melatonin mitigates salinity-induced damage in olive seedlings by modulating ion homeostasis, antioxidant defense, and phytohormone balance [J]. Physiologia Plantarum, 2021, 173(4): 1682−1694. doi: 10.1111/ppl.13589 [8] SAMI A, SHAH F A, ABDULLAH M, et al. Melatonin mitigates cadmium and aluminium toxicity through modulation of antioxidant potential in Brassica napus L [J]. Plant Biology, 2020, 22(4): 679−690. doi: 10.1111/plb.13093 [9] POSMYK M M, KURAN H N, MARCINIAK K, et al. Presowing seed treatment with melatonin protects red cabbage seedlings against toxic copper ion concentrations [J]. Journal of Pineal Research, 2008, 45(1): 24−31. doi: 10.1111/j.1600-079X.2007.00552.x [10] 赵海亮, 左璐, 马长恩, 等. 果实膨大期叶面喷施褪黑素对番茄品质的影响 [J]. 北方园艺, 2021(17):15−21.ZHAO H L, ZUO L, MA C E, et al. Effects of foliar spraying melatonin on fruit quality of tomato during fruit expansion [J]. Northern Horticulture, 2021(17): 15−21.(in Chinese) [11] 许秋怡, 陈兆晖, 季富宴, 等. 褪黑素增强盐害条件下水稻幼苗对稻瘟病的抗病能力 [J]. 安徽农业科学, 2018, 46(9):126−128.XU Q Y, CHEN Z H, JI F Y, et al. Melatonin enhancing resistance of rice seedlings to rice blast under salt stress [J]. Journal of Anhui Agricultural Sciences, 2018, 46(9): 126−128.(in Chinese) [12] ZHAO C F, YANG M, WU X, et al. Physiological and transcriptomic analyses of the effects of exogenous melatonin on drought tolerance in maize (Zea mays L. ) [J]. Plant Physiology and Biochemistry, 2021, 168: 128−142. doi: 10.1016/j.plaphy.2021.09.044 [13] 李阳, 陈静, 刘绍东, 等. 外源褪黑素对盐胁迫下棉花幼苗生长及光合特性的影响 [J]. 新疆农业科学, 2021, 58(8):1418−1426.LI Y, CHEN J, LIU S D, et al. Effects of exogenous melatonin on growth and photosynthetic characteristics of cotton seedlings under salt stress [J]. Xinjiang Agricultural Sciences, 2021, 58(8): 1418−1426.(in Chinese) [14] 李方一, 黄璜, 官春云. 作物叶面积测量的研究进展 [J]. 湖南农业大学学报(自然科学版), 2021, 47(3):274−282.LI F Y, HUANG H, GUAN C Y. Review on measurement of crop leaf area [J]. Journal of Hunan Agricultural University (Natural Sciences), 2021, 47(3): 274−282.(in Chinese) [15] SHIBAEVA T G, MAMAEV A V, SHERUDILO E G. Evaluation of a SPAD-502 plus chlorophyll meter to estimate chlorophyll content in leaves with interveinal chlorosis [J]. Russian Journal of Plant Physiology, 2020, 67(4): 690−696. doi: 10.1134/S1021443720040160 [16] 努尔凯麦尔·木拉提, 杨亚杰, 帕尔哈提·阿布都克日木, 等. 小麦叶绿素含量测定方法比较 [J]. 江苏农业科学, 2021, 49(9):156−159.NURKHEIMER M, YANG Y J, PARHATI A, et al. Comparative study on determination methods of chlorophyll content in wheat [J]. Jiangsu Agricultural Sciences, 2021, 49(9): 156−159.(in Chinese) [17] LIU Y, ZHANG J M. Lanthanum promotes bahiagrass (Paspalum notatum) roots growth by improving root activity, photosynthesis and respiration [J]. Plants, 2022, 11(3): 382. doi: 10.3390/plants11030382 [18] 王敏, 宁秋燕, 石元值. 茶树幼苗对不同浓度铝的生理响应差异研究 [J]. 茶叶科学, 2017, 37(4):356−362.WANG M, NING Q Y, SHI Y Z. Study on physiological response of tea plant(Camellia sinensis) seedlings to different aluminum concentrations [J]. Journal of Tea Science, 2017, 37(4): 356−362.(in Chinese) [19] ARNAO M B, HERNÁNDEZ-RUIZ J. Melatonin: A new plant hormone and/or a plant master regulator? [J]. Trends in Plant Science, 2019, 24(1): 38−48. doi: 10.1016/j.tplants.2018.10.010 [20] 李格, 孟小庆, 蔡敬, 等. 活性氧在植物非生物胁迫响应中功能的研究进展 [J]. 植物生理学报, 2018, 54(6):951−959.LI G, MENG X Q, CAI J, et al. Advances in the function of reactive oxygen species in plant responses to abiotic stresses [J]. Plant Physiology Journal, 2018, 54(6): 951−959.(in Chinese) [21] JIAO C J, LAN G P, SUN Y H, et al. Dopamine alleviates chilling stress in watermelon seedlings via modulation of proline content, antioxidant enzyme activity, and polyamine metabolism [J]. Journal of Plant Growth Regulation, 2021, 40(1): 277−292. doi: 10.1007/s00344-020-10096-2 [22] 蒋希瑶, 黄俊杰, 周英杰, 等. 不同浓度外源褪黑素对NaHCO3胁迫下番茄幼苗生长和生理指标的影响 [J]. 北方园艺, 2022(9):1−9.JIANG X Y, HUANG J J, ZHOU Y J, et al. Effects of exogenous melatonin on growth and physiological indexes of tomato seedlings under NaHCO3 stress [J]. Northern Horticulture, 2022(9): 1−9.(in Chinese) [23] 杜昕, 李博, 毛鲁枭, 等. 褪黑素对干旱胁迫下大豆产量及AsA-GSH循环的影响 [J]. 作物杂志, 2022(1):174−178.DU X, LI B, MAO L X, et al. Effects of melatonin on yield and AsA-GSH cycle in soybean under drought stress [J]. Crops, 2022(1): 174−178.(in Chinese) [24] 陈奋奇, 方鹏, 白明兴, 等. 外源脯氨酸缓解玉米幼苗盐胁迫的效应 [J]. 草业科学, 2022, 39(4):747−755.CHEN F Q, FANG P, BAI M X, et al. Mitigation of salt stress in maize seedlings by exogenous proline application [J]. Pratacultural Science, 2022, 39(4): 747−755.(in Chinese) [25] GUO M X, ZHANG X T, LIU J J, et al. OsProDH negatively regulates thermotolerance in rice by modulating proline metabolism and reactive oxygen species scavenging [J]. Rice, 2020, 13(1): 61. doi: 10.1186/s12284-020-00422-3 [26] 吕婷. 褪黑素对小麦铝毒的缓解作用及其机理研究[D]. 杭州: 浙江大学, 2018.LV T. The role of melatonin in alleviating aluminum toxicity in wheat(Triticum aestivum L. )genotypes differing in aluminum tolerance[D]. Hangzhou: Zhejiang University, 2018. (in Chinese) [27] HOU X L, HAN H, CAI L P, et al. Pb stress effects on leaf chlorophyll fluorescence, antioxidative enzyme activities, and organic acid contents of Pogonatherum crinitum seedlings [J]. Flora, 2018, 240: 82−88. doi: 10.1016/j.flora.2018.01.006 [28] 尹赜鹏, 王珍琪, 齐明芳, 等. 外施褪黑素对盐胁迫下番茄幼苗光合功能的影响 [J]. 生态学杂志, 2019, 38(2):467−475. doi: 10.13292/j.1000-4890.201902.023YIN Z P, WANG Z Q, QI M F, et al. Effects of melatonin application on photosynthetic function in tomato seedlings under salt stress [J]. Chinese Journal of Ecology, 2019, 38(2): 467−475.(in Chinese) doi: 10.13292/j.1000-4890.201902.023 [29] HOSSEIN P A, ÖZKAN G, BALPINAR N Ö, et al. Estimation of genomic instability and DNA methylation due to aluminum (Al) stress in wheat (Triticum aestivum L. ) using iPBS and CRED-iPBS analyses [J]. Turkish Journal of Botany, 2019, 43(1): 27−37. doi: 10.3906/bot-1804-23 [30] ACHARY V M M, PARINANDI N L, PANDA B B. Calcium channel blockers protect against aluminium-induced DNA damage and block adaptive response to genotoxic stress in plant cells [J]. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 2013, 751(2): 130−138. doi: 10.1016/j.mrgentox.2012.12.008 [31] HE S J, HU Y J, WANG H B, et al. Effects of indole-3-acetic acid on arsenic uptake and antioxidative enzymes in Pteris cretica var. nervosa and Pteris ensiformis [J]. International Journal of Phytoremediation, 2017, 19(3): 231−238. doi: 10.1080/15226514.2016.1207609 [32] NAZIR H, ASGHAR H N, AHMAD Z Z, et al. Judicious use of kinetin to improve growth and yield of rice in nickel contaminated soil [J]. International Journal of Phytoremediation, 2016, 18(7): 651−655. doi: 10.1080/15226514.2015.1094444 -
点击查看大图
图(2) / 表(6)
计量
- 文章访问数: 888
- HTML全文浏览量: 339
- PDF下载量: 23
- 被引次数: 0
