蚕豆芽苗菜高左旋多巴产量的LED光培养条件研究

李程勋; 徐晓俞; 李爱萍; 郑开斌

doi:10.19303/j.issn.1008-0384.2023.05.005

蚕豆芽苗菜高左旋多巴产量的LED光培养条件研究

doi: 10.19303/j.issn.1008-0384.2023.05.005

李程勋^1,,
徐晓俞¹,
李爱萍^1, ,,
郑开斌^2, ,

1.
福建省农业科学院作物研究所，福建　福州　350013
2.
福建省农业科学院农业生态研究所，福建　福州　350003

基金项目: 福建省公益类科研院所基本科研专项（2021R1031004）；福建省农业种质资源创新专项（2023）；福建省农业科学院科技创新团队（CXTD2021011-2）；福建省农业高质量发展超越“5511”协同创新工程项目（XTCXGC2021019）

详细信息

作者简介:
李程勋（1991−），男，硕士，助理研究员，研究方向：作物遗传品质育种与农产品天然产物提取（Email：1219513539@qq.com）

通讯作者:
李爱萍（1968−），女，研究员，研究方向：作物遗传品质育种及农产品天然产物提取（E-mail:apl909@163.com）

郑开斌（1966−），男，博士，研究员，研究方向：作物遗传品质育种及农产品天然产物提取（E-mail:k03163@163.com）

中图分类号: S 529
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- 被引次数: 0
出版历程
- 收稿日期: 2021-11-17
- 录用日期: 2022-08-03
- 修回日期: 2022-04-20
- 网络出版日期: 2023-03-06
- 刊出日期: 2023-05-28

LED-stimulated Levodopa Production in Broad Bean Sprouts

LI Chengxun^1
,,
XU Xiaoyu¹,
LI Aiping^{1
, ,},
ZHENG Kaibin^{2
, ,}

1.
Crop Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China
2.
Agricultural Ecology Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350003, China

摘要

摘要: 目的研究不同LED光培养条件对蚕豆芽苗菜功效成分左旋多巴产量的影响。方法通过单因素试验和L₉（3⁴）正交试验研究不同光强、光照时间和培养时间3个因素对蚕豆芽苗菜生长及其功效物质左旋多巴产量的影响。结果弱光促进蚕豆芽苗菜干物质的积累，强光促进蚕豆芽苗菜左旋多巴质量分数的积累，蚕豆芽苗菜高左旋多巴产量的最优培养条件为光强500 lx、光照时间9 h、培养时间6 d，在此培养条件下蚕豆芽苗菜左旋多巴产量为19.62 g·m⁻²。结论通过调节蚕豆芽苗菜的光培养条件可以提高其功效物质左旋多巴的产量，本研究为蚕豆芽苗菜的功效物质成分提取提供参考依据，有利于蚕豆芽苗菜的开发利用。
- 蚕豆芽苗菜 /
- 正交试验 /
- 光照强度 /
- 左旋多巴
Abstract: Objective Effect of LED photoculture on yield of levodopa in broad bean sprouts was studied. Method Broad bean sprouts were exposed to LED of varied intensity and cultured for varied time periods in single factor and L₉ (3⁴) orthogonal experiments. Levodopa generated in the sprouts under the conditions was determined for process optimization. Result Low LED light enhanced the accumulation of sprout dry matters. However, it was under high intensity the productivity of levodopa in the sprouts was significantly encouraged. The optimum conditions for a maximized levodopa production of 19.62 g·m⁻² were found to include 9 h of 500 lx LED exposure in 6 d of cultivation. Conclusion The photoculture of broad bean sprouts under intense LED lights could significantly promote the production of the functional amino acid, levodopa.
- Broad bean sprouts /
- orthogonal test /
- LED light intensity /
- levodopa

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图 1 培养时间对蚕豆芽苗菜的影响

Figure 1. Effect of culture time on broad bean sprouts

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图 2 光强对蚕豆芽苗菜的影响

Figure 2. Effect of LED light intensity on broad bean sprouts

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图 3 光照时间对蚕豆芽苗菜的影响

Figure 3. Effect of LED exposure time on broad bean sprouts

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表 1 因素水平

Table 1. Factors and levels of orthogonal experiment

水平 Level	因素 Factor
水平 Level	A培养时间 Culture time/d	B 光强 Light intensity/lx	C 光照时间 Light time/h
1	6	250	6
2	7	500	9
3	8	750	12

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表 2 蚕豆芽苗菜左旋多巴产量的正交试验结果与分析表

Table 2. Orthogonal test results and analysis on levodopa produced in broad bean sprouts

编号 Number	因素 Factor				左旋多巴产量 Levodopa yield/（g·m⁻²）
编号 Number	A培养时间 Culture time	B光强 Light intensity	C光照时间 Light time	空列 Empty column	左旋多巴产量 Levodopa yield/（g·m⁻²）
1	1	1	1	1	16.83
2	1	2	2	2	19.62
3	1	3	3	3	17.56
4	2	1	2	3	19.26
5	2	2	3	1	19.55
6	2	3	1	2	16.87
7	3	1	3	2	18.64
8	3	2	1	3	18.64
9	3	3	2	1	18.06
K₁	54.01	54.73	52.34	54.44
K₂	55.68	57.81	56.94	55.13
K₃	55.34	52.49	55.75	55.46
k₁	18.00	18.24	17.45	18.15
k₂	18.56	19.27	18.98	18.38
k₃	18.45	17.50	18.58	18.49
极差 Range	0.56	1.77	1.53	0.34
因素主次 Factor primary and secondary		B＞C＞A
优水平 Excellent level	A₂	B₂	C₂
优组合 Excellent combination	B₂C₂A₂

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表 3 方差分析

Table 3. Analysis of variance

因素 Factor	偏差平方和 Sum of squares of deviations	自由度 Degree of freedom	F值 F value	显著性 Significance
A	0.52	2	2.89	—
B	4.75	2	26.37	*
C	3.80	2	21.10	*
F_0.05（2，2）=19，当F＞19说明有显著性意义，^*P＜0.05。 F_0.05（2，2）=19， F ＞19 means significant difference (P＜0.05).

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