Breeding of High-quality, Wide-adaptability Super-sweet Corn, Minshuangse No. 6
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摘要:
目的 选育品质优、产量高、抗倒性强、抗病性较强、耐热性好、适应性广的超甜玉米新品种。 方法 2015年冬,应用 “温带种质×热带种质”的甜玉米杂种优势模式,利用福建省农业科学院作物研究所最新育成的热带种质黄色超甜玉米自交系闽甜系T146与温带种质白色超甜玉米自交系闽甜系AS67测交配组,培育黄白粒超甜玉米新品种闽双色6号。2018–2021年闽双色6号分别通过了国家东南区、西南区鲜食玉米科企联合体区域试验。并于2021和2022年在广东惠州和福建建瓯开展千亩示范。 结果 国家区试结果:产量方面,闽双色6号在2018-2019年东南区试中,两年平均鲜穗产量13587.8 kg·hm−2,比对照品种粤甜16号增产7.6%,增产点率80.9%。在西南区试中,两年平均鲜穗产量13505.3 kg·hm−2,比对照品种粤甜16号增产3.6%,增产点率70%。抗逆性方面,鲜食玉米品种闽双色6号在田间自然发病的情况下,中抗小斑病,中抗/高抗纹枯病,中抗/高抗瘤黑粉病,中抗茎腐病,中抗/感大斑病和南方锈病。接种鉴定结果表明,该品种感丝黑穗病,感/中抗小斑病,感瘤黑粉病,高感纹枯病,感/中抗南方锈病。品质方面,经专家鉴定,国家东南区试验综合评分89.2分,国家西南区试验综合评分86.8分,均优于对照品种粤甜16号(85.0分);经扬州大学农学院理化品质检测,闽双色6号国家东南、西南两大区试平均皮渣率为11.8%,可溶性总糖含量为26.7%,还原糖含量为11.25%,优于对照品种粤甜16号的理化品质。 分别于2020年和2022年通过国家农作物品种审定委员会审定(国审玉20200523、国审玉20220577 )。惠州市马安镇千亩示范结果显示闽双色6号每公顷产量17982 kg,比对照增产5.9%,每公顷可增效7014元,品质品尝90.8分;建瓯市东游镇示范片每公顷产量18889.5 kg,比对照增产9.44%,每公顷可增效7173.6元,品质品尝为89.7分,均优于对照,且建议扩大该品种的推广和应用。 结论 闽双色6号很好地协调了品质与产量、抗性的关系,具有品质优、产量较高、抗倒性强、抗病性较强、耐热性较好、适应性广等优点,满足了生产上对优质高产新品种的需求,适宜在我国南方鲜食玉米种植区种植;闽双色6号的选育成功,再次证明“温带种质×热带种质”的杂种优势模式是甜玉米新品种选育的有效杂种优势利用模式。 Abstract:Objective A new variety of super-sweet corn characterized with the desirable properties of high-quality, high-yield, wide-adaptability, and strong resistance to lodging, diseases, and hot climate was bred. Methods A tropical yellow-kernel, super-sweet inbred Mintian T146 line of corn germplasm was crossbred with Mintian AS67, a temperate white-kernel, super-sweet corn inbred line newly developed by the Crop Research Institute at Fujian Academy of Agricultural Sciences. The new variety of corn was submitted to regional trials followed by test production held in two regions in the nation. Results Super-sweet Minshuangse No. 6 bearing both yellow and white kernels on a cob was successfully bred in the winter of 2015. From 2018 to 2021, it was certified at the southeast and southwest regional trails for fresh consumption corn cultivars by the National Science and Enterprise Consortium. At the southeast regional trial from 2018 to 2019, Minshuangse No. 6 delivered an average fresh ear yield of 13,587.8 kg·hm−2, which was 7.6% higher than that of the reference Yuetian No. 16 with a yield point rate of 80.9%. A similar yield of 13 505.3 kg·hm−2 was shown at the southwest regional trial that was 3.6% higher than that of Yuetian 16 with a yield point rate of 70%. On the resistance to diseases under natural infectious conditions in the field, Minshuangse No. 6 was judged to be moderately resistant to the small spot disease, moderately to highly resistant to the grain wilt, moderately to highly resistant to the nodulation smuddery, moderately resistant to the stem rot, and moderately resistant to susceptible to the large spot disease and the southern rust. By artificial inoculation, it was identified to be susceptible to the head smut, susceptible to moderately resistant to the small spot, susceptible to the nodular smut, susceptible to the sheath blight, and susceptible to moderately resistant to the southern rust. In terms of quality, the comprehensive scores it received at the southeast regional trial was 89.2 and at the southwest 86.8, as compared to 85.0 for the reference. The physical and chemical quality analyses conducted by the Agricultural College at Yangzhou University indicated that the average residue percentage, total soluble sugars, and reducing sugars of the corn kernels sampled from Minshuangse No. 6 at the regional trials were 11.8%, 26.7%, and 11.25%, respectively. They were all higher than those of Yuetan 16. The new variety was officially granted certificates under the National Jade No. 20200523 in 2020 and the National jade No. 20220577 in 2022 by the National Crop Variety Certification Committee. In the 2021 and 2022 thousand-mu production tests, Minshuangse No. 6 had a production yield at Huizhou, Guangdong of 17 982 kg·hm−2, representing an increase of 5.9% over control and an improved revenue of 7 014 yuan·hm−2. And it scored higher than the reference at 90.8 on sensory evaluation. The results of the trial held at Jianou, Fujian, Minshuangse No. 6 showed a yield of 18 889.5 kg·hm-2, which was 9.44% higher than that of reference and 7 173.6 yuan·hm−2 more on revenue. It received 89.7 on the sensory score, which was also higher than the reference. Conclusion Minshuangse No. 6 displayed superior quality, yield, adaptability, and resistance to lodging, diseases, and heat. Consequently, it was recommended for promotion in the regions. Furthermore, the successful heterosis demonstrated by this study confirmed the validity of the "temperate germplasm × tropical germplasm" mode for corn breeding in general. -
Key words:
- Sweet corn /
- variety breeding /
- Minsuangse No. 6 /
- characteristics
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表 1 闽双色6号在国家东南区鲜食玉米科企联合体区试中的产量表现
Table 1. Yield of Minshuangse NO. 6 at southeast regional trial on fresh consumption corn cultivars held by National Science and Enterprise Consortium
品种
Variety年份
Year鲜穗产量
Yield/
(kg·hm−2)增产点率
Increasing point
rate/%比CK±
Than
CK±/%闽双色6号
Minshuangse NO.62018 13150.5** 80.9 8.2 2019 14025.0** 80.9 6.9 平均
Average13587.8 80.9 7.6 粤甜27号(CK)
Yuetian NO.272018 12156.0 粤甜16号(CK)
Yuetian NO.162019 13125.0 平均
Average12640.5 显著性差异分析:* P < 0.05,** P < 0.01,无标注表示无显著差异。表2同。
* indicates significant difference at P<0.05; * * significant difference at P<0.01; or no significant difference. Same for Table 2.表 2 闽双色6号在国家西南区鲜食玉米科企联合体区试中的产量表现
Table 2. Yield of Minshuangse NO. 6 at southwest regional trial on fresh consumption corn cultivars held by National Science and Enterprise Consortium
品种
Variety年份
Year鲜穗产量
Yield/
(kg·hm−2)增产点率
Increasing point
rate/%比CK±%
Than CK±/%闽双色6号
Minshuangse NO.62020 13947.0 70 3.9 2021 13063.5 70 3.2 平均
Average13505.3 70 3.6 粤甜16号(CK)
Yuetian NO.162020 13428.0 2021 12657.0 平均
Average13042.5 表 3 2018-2022闽双色6号在国家东南和西南区甜玉米区域试验中穗部性状表现
Table 3. Yield and ear characteristics of Minshuangse No. 6 in regional trials on sweet corns for southeast and southwest China from 2018 to 2022
区试地区
District
trial area年份
Year品种
Variety出苗至采收天数
Emergence to Day of
harvest/d穗长
Panicle
length/cm穗粗
Ear
thick/cm穗行数
Ear
rows秃尖
Bald
tip/cm鲜百粒重
Fresh 100 grain
weight/g鲜出籽粒
Kernel
ratio/%东南区 2018 闽双色6号
Minshuangse NO.670.5 19.7 5.0 15.6 1.5 38.0 66.5 粤甜27号
Yuetian NO.2778.7 20.3 4.9 14.6 0.9 34.6 68.3 2019 闽双色6号
Minshuangse NO.668 19.9 5.0 — 1.3 37.5 67.9 粤甜16号
Yuetian NO.1669.9 17.9 5.2 — 0.8 38.3 73.1 西南区 2020 闽双色6号 Minshuangse NO.6 82.5 20.0 5.0 15.9 1.9 40.1 70.6 粤甜16号
Yuetian NO.1685.7 17.9 5.2 17.1 0.8 40.9 73.0 2021 闽双色6号
Minshuangse NO.678.6 20.3 5.0 16.2 3.0 35.1 69.8 粤甜16号
Yuetian NO.1680.4 18.6 5.2 17.0 1.6 39.1 75.5 表 4 国家东南区鲜食玉米科企联合体区试中抗性鉴定结果
Table 4. Resistance test results on fresh consumption sweet corns in southeast region
品种
Variety年份
Year小斑病
Small spot disease纹枯病
Sheath blight瘤黑粉病
Gall smut南方锈病
Southern Rust大斑病
Large spot
disease茎腐病
Stem rot倒伏倒折率
Lodging and
inversion rate/%田间
Field接菌
Inoculation田间
Field接菌
Inoculation田间
Field接菌
Inoculation田间
Field接菌
Inoculation闽双色6号
Minshuangse NO.62018 MR S MR HS R S MR S MR MR 0.5 2019 MR MR R HS MR S S S S MR 1.2 均值
AverageMR MR MR HS MR S
SS S MR 0.9 粤甜16号
Yuetian NO.16(CK)2018 MR S MR MR R HR MR MR MR MR 0.3 2019 MR S MR MR R HS MR S R MR 1.3 均值
AverageMR S MR MR R R MR
SR MR 0.8 S:感病;MS:中感;HS:高感;R:抗病;MR:中抗;HR:高抗。表5同。
S: susceptible; HS: high susceptible; R: resistance; MR: medium resistance; HR: high resistance.Same for Table 5.表 5 国家西南区鲜食玉米科企联合体区试中抗性鉴定结果
Table 5. Resistance test results on fresh consumption sweet corns in southwest region
品种
Variety年份
Year小斑病
Small spot disease纹枯病
Sheath blight丝黑穗病
Head smut大斑病
Large spot disease瘤黑粉病
Gall smut倒伏倒折率
Lodging and inversion rate/%田间
Field接菌
Inoculation田间
Field接菌
Inoculation田间
Field接菌
Inoculation闽双色6号
Minshuangse NO.62020 MR S R HS HR HS R HR 0.8 2021 R MR HR HS HR S MR HR 0.3 均值
AverageMR MR MR HS HR S R HR 0.6 粤甜16号
Yuetian NO.16(CK)2020 MR R R HR HR 1.1 2021 MR HR HR HR HR 0.0 均值
AverageMR MR MR HR HR 0.6 表 6 国家东南区鲜食玉米科企联合体区试品质表现
Table 6. Quality of Minshuangse No. 6 evaluated at southeast regional trial
品种
Variety年份
Year专家品质品尝综合评分
The expert tasting appraisal score皮渣率
Dregs rate/%可溶性总糖
Total soluble sugar/%还原糖
Reducing sugar/%东阳
Dongyang福州
Fuzhou广州
Guangzhou平均
Average闽双色6号
Minshuangse NO.62018 86.8 92.9 87.9 89.2 12.3 29.6 15.0 2019 86.4 91.8 88.9 89.0 9.5 29.9 10 均值
Average86.6 92.4 88.4 89.1 10.9 29.8 12.5 粤甜16号(2018 CK)
Yuetian NO.162018 85.0 85.0 85.0 85.0 12.0 18.5 10.2 粤甜27号(2019 CK)
Yuetian NO.272019 85.0 85.0 85.0 85.0 8.9 14.3 7.9 表 7 国家东南区鲜食玉米科企联合体区试品质表现
Table 7. Quality of Minshuangse No. 6 evaluated at southwest regional trial
品种
Variety年份
Year专家品质品尝综合评分
The expert tasting appraisal score皮渣率
Dregs rate/%可溶性总糖
Total soluble sugar/%还原糖
Reducing sugar/%湖北
Dongyang四川
Fuzhou贵州
Guangzhou平均
Average闽双色6号
Minshuangse NO.62020 86.3 85.3 88.0 86.5 11 26.5 12.3 2021 87.3 84.0 90.0 87.1 14.5 20.7 7.8 均值
Average86.8 85.4 89.0 86.8 12.74 23.6 10.0 粤甜16号(CK)
Yuetian NO.162020 85.0 85.0 85.0 85.0 12 18.5 10.2 粤甜27号(CK)
Yuetian NO.162021 85.0 85.0 85.0 85.0 8.9 14.3 7.9 均值
Average85.0 85.0 85.0 85.0 10.5 16.4 9.1 表 8 2020、2021闽双色6号在耐热试验中的产量及穗部性状表现
Table 8. Yield and panicle characteristics of Minshuangse No. 6 shown in 2019 and 2020 heat resistance tests
年份
Year品种
Variety出苗至采收天
Emergence to
Day of harvest/d开花抽丝间隔
Anthesis- silking
interval(ASI)鲜穗产量
The yield of
fresh ear per ha/
kg·hm−2穗长
Panicle length/
cm穗粗
Ear thick/
cm秃尖
Bald tip/
cm鲜百粒重
Fresh 100
grain weight/g鲜出籽粒
Kernel
ratio/%2020 闽双色6号
Minshuangse NO.669.0 0.20 13285.5 19.8 5.0 0.9 38.2 68.5 粤甜16号
Yuetian NO.1672.5 0.30 12382.5 19.8 4.9 1.0 35.6 67.5 2021 闽双色6号
Minshuangse NO.668.0 0.10 13966.5 19.9 5.1 0.8 37.8 68.2 粤甜16号
Yuetian NO.1669.9 0.33 13113 18.1 5.0 1.1 37.3 66.9 表 9 2021年和2022年超甜玉米闽双色6号示范结果
Table 9. Performance of super-sweet corn Minshuangse No. 6 demonstrated in 2021 and 2022 trials
品种
Variety示范地点
Demonstration site核心示范
面积
Core
demonstration
area /hm2辐射面积
Radiated
area/
hm23点平均重量
Average
weight of
3 points/kg产量
Yield per
ha/
(kg·hm−2)比CK±%
Than CK±/
%品质品尝分
Quality
tasting
score田间收购
价格
Field
purchase
price/kg比CK增加的
经济效益
Increased economic
benefit over
CK(Yuan)闽双色6号
Minshuangse NO.6广东省惠州市
马安镇
Ma 'an Town,
Huizhou City,
Guangdong Province14.4 773.3 114.3 19050 5.9 90.8 3.0 7014 金百甜15号
Jinbaitian NO.15- - 107.9 17982 - 85 2.8 闽双色6号
Minshuangse NO.6福建省南平市
东游镇
Dongyou Town,
Nanping City,
Fujian Province8.4 80 136.0 18889.5 9.44 89.7 4.4 7173.6 萃甜216
Cuitian216- - 107.0 17257.5 - 87.1 4.2 -
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