水稻优良恢复系福恢676的粒形及米质分析

连玲; 周鹏; 郑菲艳; 朱永生; 郑燕梅; 王福祥; 何炜; 蔡秋华; 谢华安; 张建福

doi:10.19303/j.issn.1008-0384.2023.02.001

水稻优良恢复系福恢676的粒形及米质分析

doi: 10.19303/j.issn.1008-0384.2023.02.001

连玲^{1, 2,},
周鹏^1,,
郑菲艳¹,
朱永生^{1, 2},
郑燕梅^{1, 2},
王福祥^{1, 2},
何炜^{1, 2},
蔡秋华^{1, 2},
谢华安^{1, 2},
张建福^{1, 2, ,}

1.
福建省农业科学院水稻研究所，福建　福州　350019
2.
农业农村部华南杂交水稻种质创新与分子育种重点实验室/福州（国家）水稻改良分中心/福建省作物分子育种工程实验室/福建省水稻分子育种重点实验室/福建省作物分子育种省部共建国家重点实验室培育基地/水稻国家工程研究中心/杂交水稻国家重点实验室华南研究基地，福建　福州　350003

基金项目: 国家水稻产业技术体系建设专项（CARS-01-20）；福建省农业高质量发展超越“5511”协同创新工程项目（XTCXGC2021001）；福建省科技重大专项（2020NZ08016）；福建省科技计划公益类专项（2020R1023008）；福建省农业科学院科技创新团队建设项目（CXTD2021005-3）

详细信息

作者简介:
连玲（1983−），女，硕士，助理研究员，研究方向：水稻分子生物学与分子育种研究（E-mail：lianling51@163.com）

周鹏（1983−），男，硕士，助理研究员，研究方向：水稻遗传育种（E-mail：158544855@qq.com）

通讯作者:
张建福（1971−），男，博士，研究员，博士生导师，研究方向：水稻分子设计育种研究（E-mail:jianfzhang@163.com）

中图分类号: S511
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出版历程
- 收稿日期: 2022-11-01
- 录用日期: 2022-11-01
- 修回日期: 2023-01-07
- 网络出版日期: 2023-03-28
- 刊出日期: 2023-02-28

Grain Shape and Quality of Premium Rice Restorer Fuhui 676

LIAN Ling^{1, 2
,},
ZHOU Peng^1
,,
ZHENG Feiyan¹,
ZHU Yongsheng^{1, 2},
ZHENG Yanmei^{1, 2},
WANG Fuxiang^{1, 2},
HE Wei^{1, 2},
CAI Qiuhua^{1, 2},
XIE Huaan^{1, 2},
ZHANG Jianfu^{1, 2
, ,}

1.
Rice Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350019, China
2.
Key Laboratory of Germplasm Innovation and Molecular Breeding of Hybrid Rice for South China, Ministry of Agriculture and Rural Affairs/Fuzhou Branch, National Rice Improvement Center of China/Fujian Engineering Laboratory of Crop Molecular Breeding/Fujian Key Laboratory of Rice Molecular Breeding/Incubator of National Key Laboratory of Fujian Germplasm Innovation and Molecular Breeding between Fujian and Ministry of Sciences & Technology/National Engineering Laboratory of Rice/South Base of National Key Laboratory of Hybrid Rice of China, Fuzhou, Fujian　350003, China

摘要

摘要: 目的福恢676是福建省农业科学院水稻研究所在“丰产性、抗逆性、优质性及适应性”水稻育种策略指导下育成的强优势恢复系。对福恢676的粒形和米质进行相关研究，可以为该恢复系更好地应用于育种生产提供理论依据。方法将福恢676、亲本明恢63和蜀恢527、恢复系明恢86和福恢673种植于福州、泉州及三明试验基地，收获成熟种子，自然晒干。测量各恢复系的谷粒长、宽及厚度，称取千粒重，测定各恢复系的米质，并进行比较分析。采用CTAB法提取水稻基因组DNA，PCR扩增Wx基因片段，采用限制性内切酶Acc I对PCR产物进行酶切分析，根据琼脂糖电泳结果及测序分析确定Wx基因第一内含子+1位碱基类型。采用Trizol法提取水稻种子总RNA，进一步采用SYBR Green I荧光定量PCR（qRT-PCR）分析灌浆不同时期控制粒形和米质相关基因的表达情况。结果福恢676的谷粒长大多为10.0~11.0 mm，宽约2.70 mm，厚约2.00 mm，千粒重27.0~31.0 g。福恢676的糙米率大于81.0%，胶稠度大于60.0 mm，直链淀粉含量在13.0%~18.0%，达到一级米标准；福恢676的整精米率高于其他4个恢复系，垩白率和垩白度高于亲本明恢63与蜀恢527，低于明恢86和福恢673。福恢676和其他4个恢复系中Wx基因第一内含子+1位碱基均为T，即Wx基因型为Wx^b。福恢676中控制粒形相关基因GL7的表达在灌浆11 d时明显下降； GS3、SGL和GIF1的表达先升高后降低，且在灌浆7 d时表达量最高；GW8的表达先降低后升高，且在灌浆11 d时表达量最高。福恢676中控制米质相关基因Chalk5的表达量在灌浆7 d时最高，而在11 d时几乎不表达；ALK、OsSSI、OsBEIIb的表达先升高后降低，在灌浆7 d时表达量最高，且表达模式与其他4个恢复系中的完全不同。结论福恢676的谷粒较长，整精米率较高，糙米率、胶稠度和直链淀粉含量达到一级米标准，Wx基因型为Wx^b，福恢676中控制粒形基因GL7、GS3和米质基因ALK、OsSSI 、OsBEIIb的表达模式与其他4个恢复系中有明显不同。
- 福恢676 /
- 粒形 /
- 米质 /
- Wx基因 /
- 基因表达分析
Abstract: Objective Shape and quality of the grains of Fuhui 676, a premium high-yield, high-quality, stress resistant, highly adaptable restorer of rice, were studied for further improvements. Method Along with Fuhui 676, which was previously bred by the Rice Research Institute at Fujian Academy of Agricultural Sciences, Minghui 63, Shuhui 527, Minghui 86, and Fuhui 673 were planted at the experimental stations in Fuzhou, Quanzhou, and Sanming. At maturation, grains were harvested and sun-dried to measure the length, width, thickness, 1000-grain weight, and quality. Genomic DNA of the plants was extracted by CTAB method; Wx fragments amplified by PCR; restriction enzymes analyzed by means of Acc I digestion; polymorphism of the first base of Wx gene intron 1 determined by agarose gel electrophoresis and sequence analysis; total RNA of the grains extracted by Trizol method; and expressions of the grain-shape-and-quality-related genes at different grain-filling stages analyzed by SYBR Green I fluorescent quantitative PCR (qRT-PCR). Result In general, the Fuhui 676 grains measured 10–11 mm long, 2.70 mm wide, 2.00 mm thick, and 27.0–31.0 g per 1000-grains. After dehulling, they yielded more than 81% brown rice with a gel consistency of more than 60.0 mm and an amylose content in the range of 13%–18%, which met the 1^st grade standard. The grains had a higher head rice rate than those of the other 4 restorer lines; and the chalkiness rate and degree higher than those of the parents, Minghui 63 and Shuhui 527, but lower than those of Minghui 86 and Fuhui 673. The first base of Wx gene intron 1 in the restorers was T, i.e., of the Wx^b genotype. The expression of GL7 related to grain shape in Fuhui 676 decreased significantly on the 11 d of grain-filling, while those of GS3, SGL, and GIF1 increased initially and then decreased with a peak appeared on the 7 d; that of GW8 decreased at first and then increased to the highest level on the 11 d; that of the quality-related Chalk5 reached the maximum on the 7 d but nearly dissipated on the 11 d; and those of ALK, OsSSI, and OsBEIIb rose to a peak on the 7 d and followed by a decline. The expression patterns of those genes in Fuhui 676 were completely different from those in the other 4 restorers. Conclusion Fuhui 676 produced long grains of high head milled rice rate, brown rice rate, gel consistency, and amylose content of 1^st grade rice. The Wx genotype of Fuhui 676 was Wx^b. The expression patterns of GL7, GS3 and ALK, OsSSI, OsBEIIb related to grain shape and quality in Fuhui 676 were distinctively different from those of the other tested restorer lines.
- Fuhui 676 /
- grain shape /
- grain quality /
- Wx gene /
- gene expression

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图 1 不同恢复系稻谷粒形（福州）

Figure 1. Shapes of rice grains of different restorer lines (Fuzhou)

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图 2 Wx基因片段的PCR扩增及限制性酶切分析

A为PCR扩增，B为限制性酶切分析；M为Marker 2000，1为对照广恢128，2为明恢86，3为明恢63，4为福恢676，5为福恢673，6为蜀恢527。

Figure 2. PCR amplification and restriction enzyme analysis of Wx gene fragment

A: PCR amplification; B: restriction enzyme analysis; M: Marker 2000 bp; 1: Guanghui128 as control; 2: Minghui86; 3: Minghui63; 4: Fuhui676; 5: Fuhui673; 6: Shuhui527.

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图 3 Wx基因片段的序列比对分析

方框内为碱基差异位置。

Figure 3. Sequence alignment on Wx gene fragment

Position of base difference is showed in box.

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图 4 控制粒形相关基因表达情况

Figure 4. Expressions of genes related to grain shape

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图 5 控制米质相关基因表达情况

Figure 5. Expression of genes related to grain quality

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表 1 qRT-PCR引物

Table 1. Primers for qRT-PCR

基因名称Gene names	引物序列（5′-3′）Primer sequence（5′-3′）
GL7	F: AAGGACTTCAGGGCTCTCAGGATAC
GL7	R: GCTGGAAGTGTCTGGAACTGGTGTT
GS3	F: GGGTGAAATAAATTCAATCGAAGGG
GS3	R: GCACAAACAGCGAAACTTCTTCAAG
GW8	F: AGGAGTTTGATGAGGCCAAG
GW8	R: GCGTGTAGTATGGGCTCTCC
SGL	F: CTCTTCTATGGAACCTGACAG
SGL	R: CTGAGAAGCTGAAGCAGATG
GIF1	F: TTGGTAGTAGGGTCGCTTGGGC
GIF1	R: CCAGTCCGGAGCTCAGAGTCGA
Chalk5	F: GCCGTCACCTTCCTCTCCCTCC
Chalk5	R: CGTAAGCATTGCTCGTGAAGTACTCG
ALK	F: CCTATTCCTGCGGTAGAAGA
ALK	R: CCGAATCGTCATCCTGGT
OsSSI	F: GTGAGCAGGAGTCTGAGAT
OsSSI	R: TGACCACGAAGAGCAAGA
OsBEIIb	F: CGGTTTCAGCAGGTTCAGA
OsBEIIb	R: CTCCAGATGACTCAATCTCAACTT
Actin150	F: AGTGTCTGGATTGGAGGAT
Actin150	R: TCTTGGCTTAGCATTCTTG

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表 2 稻谷粒形和千粒重分析

Table 2. Grain shape and 1000-grain weight of rice restorers

种植地区Growing areas	恢复系Restorer lines	谷粒长Grain length/mm	谷粒宽Grain width/mm	长宽比Length-width ratio	谷粒厚Grain thickness/mm	千粒重1000-grain weight/g
福州 Fuzhou	福恢676 Fuhui676	10.57±0.33	2.72±0.10	3.90±0.18	2.09±0.07	27.66±0.47
	明恢63 Minghui63	9.98±0.27*	2.72±0.09	3.67±0.15*	1.99±0.05*	26.82±0.17*
	蜀恢527 Shuhui527	11.28±0.34*	2.73±0.11	4.14±0.19*	2.08±0.06	29.40±0.13*
	明恢86 Minghui86	9.91±0.24*	2.75±0.09	3.60±0.11*	1.94±0.10*	26.60±0.20*
	福恢673 Fuhui673	10.15±0.36*	2.74±0.08	3.70±0.13*	2.00±0.07*	26.42±0.26*
泉州 Quanzhou	福恢676 Fuhui676	10.80±0.40	2.76±0.07	3.92±0.15	2.04±0.09	30.31±0.02
	明恢63 Minghui63	10.51±0.25*	2.79±0.07	3.77±0.10*	2.00±0.07	30.17±0.13
	蜀恢527 Shuhui527	11.54±0.39*	2.79±0.09	4.14±0.15*	2.00±0.09	31.82±0.01*
	明恢86 Minghui86	9.98±0.28*	2.78±0.10	3.60±0.14*	2.06±0.07	29.44±0.38*
	福恢673 Fuhui673	10.31±0.42*	2.74±0.11	3.77±0.22*	2.08±0.06	30.35±0.29
三明 Sanming	福恢676 Fuhui676	10.36±0.46	2.79±0.09	3.72±0.21	2.07±0.05	29.13±0.17
	明恢63 Minghui63	9.84±0.33*	2.80±0.09	3.52±0.18*	1.99±0.08*	28.52±0.14*
	蜀恢527 Shuhui527	10.85±0.32*	2.79±0.09	3.90±0.19*	2.01±0.08*	30.87±0.20*
	明恢86 Minghui86	9.44±0.29*	2.85±0.12	3.32±0.19*	1.94±0.08*	27.88±0.48*
	福恢673 Fuhui673	9.99±0.40*	2.86±0.10*	3.49±0.16*	1.97±0.11*	26.89±0.38*
表示差异达显著水平（P≤ 0.05），没有标注表示没有显著差异。下表同。*indicate significant differences at P≤0.05. Same for below.

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表 3 米质分析

Table 3. Analysis of grain quality

种植地区Growing Areas	恢复系Restorer lines	糙米率Brown rice rate/%	精米率Milled rice rate/%	整精米率Head rice rate/%	长Length/mm	宽Width/mm	长宽比Length to width	垩白率Chalkiness ratio/%	垩白度Chalkiness degree/%	透明度Transparency	碱消值Alkali value	直链淀粉含量Amylose content/%	胶稠度Gel consistency/mm
福州Fuzhou	福恢676　Fuhui676	81.40±0.13	69.60±0.28	44.10±0.59	6.39±0.11	2.23±0.09	2.88±0.02	28.13±0.97	6.23±0.87	3.00±0.00	4.53±0.12	14.20±0.33	91.97±0.29
	明恢63　Minghui63	81.80±0.26	69.80±0.96	38.27±1.06*	6.39±0.19	2.30±0.02	2.80±0.04*	18.83±1.30*	3.70±0.50*	3.00±0.00	4.30±0.10	11.97±0.31*	90.97±1.16
	蜀恢527　Shuhui527	81.87±0.64	69.30±0.79	35.10±0.56*	6.95±0.25*	2.30±0.06	3.12±0.07*	16.87±0.38*	3.23±0.15*	2.00±0.00	4.43±0.06	12.47±0.12*	86.00±0.80*
	明恢86　Minghui86	82.13±0.15*	68.37±0.50*	43.10±0.79	6.19±0.12*	2.32±0.09	2.70±0.06*	35.97±1.22*	9.93±0.38*	2.00±0.00	6.83±0.12*	13.50±0.17*	83.00±0.60*
	福恢673　Fuhui673	82.23±0.15*	68.10±0.44*	41.60±0.90*	6.23±0.13	2.29±0.02	2.74±0.01*	49.57±1.76*	10.10±0.26*	3.00±0.00	4.27±0.15	12.17±0.12*	95.00±0.30*
泉州Quanzhou	福恢676　Fuhui676	81.80±0.15	71.23±0.31	55.43±0.90	6.71±0.15	2.27±0.07	2.98±0.07	16.30±0.82	3.60±0.46	2.00±0.00	4.33±0.21	15.40±0.53	88.00±0.61
	明恢63　Minghui63	82.80±0.15*	71.37±0.51	40.60±0.30*	6.83±0.10	2.38±0.05	2.90±0.04	14.13±0.76*	2.57±0.31*	3.00±0.00	4.37±0.21	13.00±0.20*	90.03±0.06*
	蜀恢527　Shuhui527	81.8±0.17	70.77±0.23	42.67±0.21*	7.23±0.21*	2.27±0.06	3.21±0.04*	11.60±0.61*	1.83±0.31*	2.00±0.00	4.30±0.26	14.23±0.38*	86.00±0.75*
	明恢86　Minghui86	82.60±0.15*	70.63±0.15*	36.80±0.17*	6.62±0.15	2.40±0.04*	2.79±0.07*	38.63±0.49*	9.07±0.70*	2.00±0.00	6.47±0.29*	15.16±0.55	78.00±0.72*
	福恢673　Fuhui673	81.70±0.20	70.60±0.20*	42.33±0.47*	6.83±0.25	2.34±0.07	2.94±0.05	50.93±3.23*	10.23±0.83*	3.00±0.00	4.20±0.10	13.57±0.21*	93.03±0.21*
三明Sanming	福恢676　Fuhui676	82.70±0.10	73.37±0.21	51.80±0.70	6.63±0.10	2.28±0.05	2.92±0.06	17.9±1.42	3.67±0.31	2.00±0.00	4.43±0.15	16.67±0.32	92.00±0.50
	明恢63　Minghui63	81.90±0.06*	71.63±0.15*	44.30±0.44*	6.75±0.19	2.41±0.08	2.81±0.07	9.20±0.56*	1.73±0.49*	2.00±0.00	4.83±0.06*	16.17±0.15	86.03±0.74*
	蜀恢527　Shuhui527	81.90±0.35*	72.10±0.40*	34.10±0.44*	7.23±0.21*	2.36±0.05	3.14±0.09*	12.80±2.26*	2.83±0.55	3.00±0.00	4.43±0.32	15.83±0.06*	91.97±1.25
	明恢86　Minghui86	83.20±0.26*	74.07±0.23*	38.67±1.26*	6.32±0.12*	2.39±0.10	2.65±0.05*	18.30±1.87	5.43±0.25*	2.00±0.00	6.83±0.21*	16.07±0.51	81.97±0.64*
	福恢673　Fuhui673	82.40±0.10*	71.03±0.21*	49.20±0.61*	6.48±0.07*	2.34±0.08	2.79±0.02*	29.73±0.80*	6.53±0.55*	3.00±0.00	4.33±0.25	14.43±0.31*	92.00±0.40

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