不同病毒侵染种根对太子参生长及产量和品质的影响

王丁; 郑伯琴; 张余; 曾令锋; 王振华; 叶祖云

doi:10.19303/j.issn.1008-0384.2023.01.009

不同病毒侵染种根对太子参生长及产量和品质的影响

doi: 10.19303/j.issn.1008-0384.2023.01.009

王丁^{1, 2,},
郑伯琴¹,
张余³,
曾令锋¹,
王振华^1, ,,
叶祖云¹

1.
宁德师范学院生命科学学院/福建省特色药用植物工程技术研究中心，福建　宁德　352100
2.
福建农林大学农学院，福建　福州　350002
3.
福建中医药大学药学院，福建　福州　350122

基金项目: 国家重点研发计划中药现代化专项（2019YFC1710500）；中央引导地方科技发展专项（2021L3030）；福建省自然科学基金项目（2021J05256）；宁德师范学院科研项目（2019FZ04、 2020FZ07、 2021ZX401、2022ZX01）；全国大学生创新创业训练计划项目（S202110398024）

详细信息

作者简介:
王丁（1997−），女，硕士研究生，主要从事药用植物脱病毒培养研究（E-mail：wangdinghd@163.com）

通讯作者:
王振华（1991−），男，副教授，主要从事植物生理生态学、药用植物生态学研究（E-mail：eco_zhwang@163.com）

中图分类号: S 567.5
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- 文章访问数: 634
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- 被引次数: 0
出版历程
- 收稿日期: 2022-09-14
- 修回日期: 2022-10-30
- 网络出版日期: 2023-02-08
- 刊出日期: 2023-01-28

Growth, Yield, and Quality of Pseudostellaria heterophylla Affected by Different Viruses Infecting Seed Roots

1.
School of Life Sciences, Ningde Normal University/Engineering Technology Research Center of Characteristic Medicinal Plants of Fujian, Ningde, Fujian　352100，China
2.
College of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
3.
School of Pharmacy, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian　350122, China

摘要

摘要: 目的探明不同病毒对太子参生长、产量及品质的影响。方法以柘参2号为试验材料，采用RT-PCR分子鉴定方法筛选出只携带一种常见病毒、携带两种常见病毒和不带病毒的太子参个体，经扩繁后，在田间使用随机区组的方法种植，探讨不同种类病毒对太子参的生长、产量和品质的影响。结果与未带病毒的植株相比，同时感染芜菁花叶病毒和蚕豆萎蔫病毒的植株总叶绿素含量、净光合速率和地上部株高分别降低了24%、33%和26%，单位面积的块根产量、浸出物含量和多糖含量分别降低了77.5%、76.2%和87.3%；而仅感染蚕豆萎蔫病毒的植株与未带病毒的植株相比，总叶绿素含量、净光合速率、地上部株高、块根产量、浸出物含量和多糖含量均无显著性差异。结论芜菁花叶病毒显著抑制太子参的生长，降低了太子参产量和品质，而蚕豆萎蔫病毒的影响不显著，在太子参脱病毒培养和脱毒苗的生产应用中应优先去除芜菁花叶病毒。
- 太子参 /
- 病毒 /
- 光合作用 /
- 产量 /
- 品质
Abstract: Objective Effects on the photosynthesis, tuber yield, and tuber quality of Pseudostellaria heterophylla plants induced by infecting viruses were examined. Methods Identified by RT-PCR, virus-free P. heterophylla Zhenshen 2 plants and those infected by turnip mosaic virus (TMV) and/or broad bean wilt virus (BBWV) were propagated in the field in a random block experiment. Leaf photosynthesis as well as yield and quality of the medicinal roots of the plants were measured. Results The total chlorophyll content, net photosynthetic rate, and height of the plants infected by both TMV and BBWV were 24%, 33%, and 26%, respectively, and the tuber yield, water-soluble extracts, and polysaccharides on a per unit area basis 77.5%, 76.2%, and 87.3%, respectively, lower than those of the virus-free counterparts. But no significant effects on any of those monitored properties were detected in the plants infected by BBWV alone. Conclusion Unlike BBWV, TMV infection on P. heterophylla significantly reduced the photosynthesis, tuber yield, and tuber quality of the plants. Thus, it was imperative to avoid TMV contamination in handling P. heterophylla seedlings.
- Pseudostellaria heterophylla /
- virus /
- photosynthesis /
- yield /
- quality

HTML全文

图 1 不同病毒处理下太子参小区生长情况

A：CK植株；B：BB植株；C：TB植株；D：CK与TB植株生长情况（5月下旬）。

Figure 1. Growth of P. heterophylla plants with or without viral infection

A: Virus-free control (CK); B: BBWV-infected plant; C: TMV-infected plant; D: Growth of CK and TMV-infected plants in late May.

下载: 全尺寸图片幻灯片

图 2 不同病毒处理下太子参生理特性的比较

A: 总叶绿素含量；B：净光合速率。数据表示为平均值±标准误差，附不同小写英文字母表示差异显著（P ＜ 0.05），下同。

Figure 2. Physiological characteristics of P. heterophylla plants with or without viral infection

A. Total chlorophyll contents ; B.Net photosynthetic rates . Data are expressed as mean ± standard error; those with lowercase letters represent significant difference ( P< 0.05). Same for below.

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图 3 不同病毒处理下太子参地上和地下生长比较

A：株高；B：单根块根直径；C：单根块根重量。

Figure 3. Growth of P. heterophylla plants with or without viral infection

A: Height of plants; B: Diameter of individual tuber; C: Weight of individual tuber.

下载: 全尺寸图片幻灯片

图 4 不同病毒处理太子参产量比较

A：块根鲜品产量；B：块根干品产量。

Figure 4. Yields of P. heterophylla plants with or without viral infection

A: Yields of fresh tubers;B:Yields of dried tubers.

下载: 全尺寸图片幻灯片

图 5 不同脱毒处理太子参品质的比较

A：浸出物质量分数；B：单位面积浸出物产量；C：多糖质量分数；D：单位面积多糖产量。

Figure 5. Quality of P. heterophylla tubers from plants with or without viral infection

A: Extracts from tubers;B: Per unit area extracts of tubers; C: Polysaccharides in tubers; D: Per unit area yield on polysaccharides of tubers.

下载: 全尺寸图片幻灯片

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