基于SPAD值的春石斛假鳞茎成熟度无损判定模型

温碧柔; 蔡雪妍; 杨逸; 彭杰良; 陈方尧; 闻真珍; 刘伟

doi:10.19303/j.issn.1008-0384.2022.06.013

基于SPAD值的春石斛假鳞茎成熟度无损判定模型

doi: 10.19303/j.issn.1008-0384.2022.06.013

1.
华南农业大学生命科学学院，广东　广州　510642
2.
西安交通大学公共卫生学院，陕西　西安　710049
3.
广东工程职业技术学院，广东　广州　510000

基金项目: 广东省重点研发计划项目（2019B20214001）；国家自然科学基金项目（30471223）；广东省自然科学基金项目（04020574）

详细信息

作者简介:
温碧柔（1996-），女，硕士研究生，研究方向：植物学（E-mail：702019335@qq.com）

通讯作者:
刘伟（1964-），男，博士，教授，研究方向：植物资源利用（E-mail：liuwei@scau.edu.cn）

中图分类号: S 682.31
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- 被引次数: 0
出版历程
- 收稿日期: 2021-12-02
- 修回日期: 2022-04-26
- 刊出日期: 2022-06-28

Noninvasive Determination on Maturity of Dendrobium nobile Pseudobulbs based on SPAD Measurements

1.
College of Life Sciences, South China Agricultural University, Guangzhou, Guangdong　510642, China
2.
School of Public Health, Xi'an Jiaotong University, Xi'an, Shaanxi　710049, China
3.
Guangdong Engineering Vocational and Technical College, Guangzhou, Guangdong　510000, China

摘要

摘要: 目的春化处理诱导开花效果不稳定是春石斛生产中经常出现的现象，其原因是气候差异导致的假鳞茎成熟的具体时间不一致，春化起始时间难于把握。为准确判断春石斛假鳞茎的成熟度，确定春化适宜的起始时间，建立了本模型。方法以大龙虾（Dendrobium Hot Lobster）、Dendrobium Sakura Hime和甜心（Dendrobium Lucky Girls ‘Sweet heart’）3个春石斛品种为材料，测定不同成熟度春石斛假鳞茎自止叶向下的5个叶位叶片的叶绿素相对含量值（SPAD），通过该5个叶位SPAD值构建的函数关系建立了与假鳞茎成熟度的相关关系模型。以可爱河马（Dendrobium Lovly Hippo）及Dendrobium Sakura Hime 2个春石斛品种为材料，统计了经低温春化后假鳞茎的开花情况，以验证该模型的有效性。结果 5个叶位叶片的叶的SPAD值之间的函数关系的k值（差异指数）与春石斛假鳞茎的成熟度相关，所构建的模型能够预测不同春化起始时间的春石斛开花情况，可用于春石斛适宜春化起始时间的判断。结论本研究提出的基于叶位和SPAD值构建的春石斛假鳞茎成熟度无损判定模型，是一种无损、快速、准确的方法，可提高春石斛生产中盆花的品质，也为春石斛精准调控栽培技术的建立奠定基础。
- 春石斛 /
- 春化效果 /
- SPAD值 /
- 春化作用 /
- 止叶
Abstract: Objective A mathematic model for accurate and noninvasive but oftentimes difficult determination on the maturation stages of orchid pseudobulbs was established for effective and timely vernalization of Dendrobium nobile. Methods Chlorophyll SPAD measurements of 5 leaves from the stop leaf to the lower ones on a pseudobulb of varieties of D. nobile including Hot Lobster, Sakura Hime, and Lucky Girls Sweetheart orchids at different stages of maturity were obtained. A correlation between the measurements and pseudobulb maturity was established. Flowering of Lovely Hippo and Sakura Hime pseudobulbs after low temperature vernalization was monitored for model verification. Results The k value (difference index) on the functional relationship between SPAD data and pseudobulb maturity indicated a viable predictability of the established model. Conclusion The newly established method for determining the maturation stages of D. nobile pseudobulbs based on SPAD measurements of differently positioned leaves was noninvasive, rapid, and accurate. The application would significantly facilitate the operation of the orchid cultivation.
- Dendrobium nobile /
- vernalizing efficiency /
- SPAD measurement /
- vernalization /
- stop leaf

HTML全文

图 1 春石斛的假鳞茎及叶片发育过程

从左到右表示春石斛不同发育阶段假鳞茎形态及叶片叶位和状态（颜色）；A指止叶；B、C、D和E为止叶下部的叶片及叶位。

Figure 1. Development of pseudobulb and leaves of D. nobile

From left to right: shape, leaf position, and leaf color of a pseudobulb at development stages；A: stop blade；B，C，D, and E: leaves and positions below stop blade.

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图 2 大龙虾（D. Hot Lobster）5个叶位叶片的SPAD值随叶龄的变化趋势（n=3）

A、B、C、D、E指由止叶向下的5片叶；实线指不同叶位叶片SPAD值；虚线为对应叶位SPAD值变化的趋势线。同一折线不同小写字母表示不同叶龄间差异显著（P˂0.05）。图3~4同。

Figure 2. Variation trend of SPAD measurements on 5 top positioned leaves of Hot Lobster pseudobulb (n=3)

A，B，C，D, and E: 5 leaves from stop leaf down；solid line: variation curve of SPAD measurements on leaves at different positions；dotted line: linear trend corresponding to change curve of SPAD measurements on counterpart leaves. Different lowercase letters indicate significant difference among different leaf age(P˂0.05). Same for Fig 3–4.

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图 3 甜心（D. Lucky Girls ‘Sweet heart’）5个叶位叶片的SPAD值随叶龄的变化趋势（n=3）

Figure 3. Variation trend of SPAD measurements on 5 top positioned leaves of Lucky Girls Sweetheart pseudobulb (n=3)

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图 4 D. Sakura Hime 5个叶位叶片的SPAD值随叶龄的变化趋势（n=3）

Figure 4. Variation trend of SPAD measurements on 5 top positioned leaves of Sakura Hime pseudobulb (n=3)

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图 5 不同生长阶段春石斛的5个叶位叶片SPAD值之间的函数关系（n=3）

实线和虚线分别为春石斛5个叶位的叶片在止叶叶龄分别为10 d、100 d和190 d的SPAD值和线性趋势线；不同小写字母表示不同叶位之间差异显著（P˂0.05）。

Figure 5. Functional relationship between SPAD measurements on 5 top positioned leaves and maturity of D. nobile pseudobulb (n=3)

Solid line and dotted line represent the SPAD variation and the linear trend of the leaves of the five leaf positions of D. nobile at the leaf age of 10, 100 and 190 d, respectively. Different lowercase letters indicate significant difference among different leaf positions (P˂0.05).

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图 6 6月至11月可爱河马石斛前5个叶位SPAD值的差异指数的变化（n=3，R²=0.7889）

Figure 6. Variability of difference index on SPAD measurements between 5 top positioned leaves of Lovely Hippo pseudobulb from June to November (n=3，R²=0.7889)

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表 1 可爱河马（D. Lovly Hippo）春化开始时假鳞茎的差异指数与春化效果（n=3）

Table 1. Difference index on Lovely Hippo pseudobulb before vernalization and flowering after vernalization (n=3)

止叶展开时间 Stop blade formation time	差异指数 Difference index（k）	开花节数比率 Ratio of flowering nodes /%
10 月	2.62±0.39 a	0.00±0.00 b
8 月	1.41±0.12 b	42.34±2.91 a
不同小写字母表示处理间差异显著（P＜0.05）。表2同。 Data with different lowercase letters indicate significant differences at P＜0.05. Same for Table 2.

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表 2 D. Sakura Hime和可爱河马石斛差异指数与春化效果（n=3）

Table 2. Difference index on Sakura Hime, Lovely Hippo, and Lucky Girls Sweetheart pseudobulbs before vernalization and flowering after vernalization (n=3)

品种 Stain	组别 Group	差异指数 Difference index（k）	总开花数 Total flowers	开花节数比率 Ratio of flowering nodes /%	每花芽开花数 Number of flowers per flower bud
D. Sakura Hime	组1	1.93±0.13 a	0.00±0.00 b	0.00±0.00 c	0.00±0.00 b
	组2	1.77±0.40 a	4.67±0.47 b	12.35±1.17 b	2.67±0.19 a
	组3	1.50±0.38 a	15.22±3.84 a	38.38±4.01 a	2.45±0.24 a
可爱河马石斛 D. Lovly Hippo	组1	1.95±0.38 a	6.11±0.45 b	27.26±2.14 c	2.07±0.11 b
	组2	1.59±0.37 a	8.78±0.28 b	36.70±1.94 b	2.12±0.12 a
	组3	0.45±0.41 a	14.00±0.90 a	45.16±2.41 a	2.48±0.12 a

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