Effect of Foliar Fertilizer Application on Loquat Fruit Quality
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摘要:
目的 研究喷施不同叶面肥对枇杷果实品质的影响,为生产栽培中改善果实品质提供技术支撑。 方法 以5-8-1枇杷为材料,在果实采前30d进行不同叶面肥(清水、氨基酸500倍液、微生物菌肥200倍液、0.6%磷酸二氢钾、0.1%钼酸铵和5 g·L−1蔗糖)6种喷施处理,测定处理后枇杷果实色差、单果重、硬度、可溶性固形物、可溶性糖、可滴定酸、Vc、酚类物质、抗氧化活性等指标。 结果 与清水对照相比,5种叶面肥处理后均能提升枇杷的单果重、可溶性固形物、可溶性糖、类胡萝卜素含量和糖酸比,同时降低可滴定酸的含量;除氨基酸处理外,对Vc含量也有显著性的提升;除磷酸二氢钾、钼酸铵处理外,果皮的a*、b*值也均有一定的提升。叶面肥喷施都显著性提升了枇杷果实总酚、总黄酮含量,其中以微生物菌肥提升的效果最明显;氨基酸、磷酸二氢钾、钼酸铵、微生物菌肥处理后的DPPH分别提高了20.92%、17.44%、17.10%、21.26%,FRAP分别提高了40.26%、40.56%、56.55%、68.05%。 结论 5种叶面肥喷施处理均能在一定程度上提升5-8-1枇杷果实外在与内在品质,其中微生物菌肥200倍液的综合效果最佳。 Abstract:Objective To study the effect of spraying different foliar fertilizers on loquat fruit quality, and provide technical support for improving fruit quality in production and cultivation. o study the effect of Spraying Different Foliar Fertilizers on loquat fruit quality, and provide technical support for improving fruit quality in production and cultivation. Method On 5-8-1 loquat plants, solutions of foliar fertilizers (i.e., 500-fold dilution of an amino acid, 200-fold dilution of a microbial fertilizer, 0.6% potassium dihydrogen phosphate, 0.1% ammonium molybdate, and sucrose at a 5 g·L−1 application rate) and water as control were separately sprayed on the leaves 30 d before harvest. The individual weight, firmness, chromatic aberration, and contents of soluble solids, soluble sugars, titratable acid, vitamin C, and total phenols as well as antioxidant capacity of the loquat fruits at harvest were measured. Result The spraying of foliar fertilizers increased the loquat fruit weight, soluble solids, soluble sugar, carotenoids, and sugar-acid ratio but reduced the titratable acid as compared with control. Except for the amino acid, all applications also significantly increased the Vc content. And aside from the potassium dihydrogen phosphate and ammonium molybdate treatments, the spraying enhanced the peel colorimetric a* and b*. The microbial fertilizer significant increased total phenols and flavonoids in the fruit, while the amino acid, potassium dihydrogen phosphate, ammonium molybdate, and microbial fertilizer, respectively, produced 20.92%, 17.44%, 17.10%, and 21.26% higher DPPH and 40.26%, 40.56%, 56.55%, and 68.05% more FRAP over control. Conclusion The 5 foliar fertilizing agents tested in the study upgraded the loquat fruit quality to varying extends. Overall, the application using a 200-fold dilution of the microbial fertilizer was recommended. -
Key words:
- Foliar fertilizer /
- loquat /
- fruit quality /
- antioxidant activity
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表 1 不同处理对枇杷果实色差的影响
Table 1. Effect of treatments on color of loquat fruit peel
处理
TreatmentL*值
L* valuea*值
a* valueb*值
b* value果皮
Peel果肉
Flesh果皮
Peel果肉
Flesh果皮
Peel果肉
Flesh清水
Fresh water68.14 ± 0.56ab 65.06 ± 0.20a 13.40 ± 0.37bc 12.54 ± 0.50a 50.25 ± 0.66ab 43.88 ± 1.06a 氨基酸
Amino acid68.84 ± 0.65a 63.37 ± 0.46a 14.61 ± 0.56ab 12.50 ± 0.55a 52.02 ± 0.73a 42.10 ± 0.87a 磷酸二氢钾
Potassium dihydrogen phosphate69.65 ± 0.50a 63.04 ± 0.90a 12.83 ± 0.58c 12.79 ± 0.46a 52.13 ± 0.53a 43.68 ± 0.94a 蔗糖
Sucrose69.32 ± 0.56a 64.55± 0.94a 14.51 ± 0.42ab 12.46 ± 0.31a 50.42 ± 0.79ab 43.21 ± 0.68a 钼酸铵
Ammonium molybdate66.28 ± 1.08b 62.86 ± 0.24a 13.93 ± 0.50abc 11.92 ± 0.45a 49.17± 1.11b 41.46± 0.86a 微生物菌肥
Microbial fertilizer67.51 ±0.76ab 64.20 ± 0.45a 15.23 ± 0.59a 12.38 ± 0.42a 52.42 ± 0.89a 43.30 ± 0.76a 同列数据后不同小写字母表示处理间差异显著(P<0.05);下同。
Data with different lowercase letters on same column indicate significant difference at P<0.05. Same for following tables.表 2 不同处理对枇杷果实品质的影响
Table 2. Effect of treatments on loquat fruit quality
处理
Treatment单果重
Weight/g硬度
Hardness/N可溶性固形物含量
TSS/%可溶性糖含量
Solublesugar/%可滴定酸含量
Soluble acid/%糖酸比
Sugar-acid ratio/%清水
Fresh water45.72 ± 0.89c 12.44 ± 0.55b 9.96 ± 0.08c 7.16 ± 0.08b 0.41 ± 0.00ab 17.68 ± 0.04c 氨基酸
Amino acid51.90 ± 2.47b 12.36 ± 0.34b 10.49 ± 0.09bc 7.55 ± 0.24b 0.39 ± 0.00a 19.44 ± 0.65b 磷酸二氢钾
Potassium dihydrogen phosphate50.34 ± 2.14bc 9.42 ± 0.36d 11.97 ± 0.30a 8.30 ± 0.23a 0.36 ± 0.00a 23.39 ± 0.71a 蔗糖
Sucrose51.26 ± 1.27b 10.66 ± 0.31c 10.63 ± 0.27b 7.72 ± 0.15ab 0.38 ± 0.01ab 20.65 ± 0.43b 钼酸铵
Ammonium molybdate49.76 ± 1.95bc 11.21 ± 0.61bc 11.09 ± 0.22b 8.25 ± 0.32a 0.37 ± 0.00a 22.94 ± 0.69a 微生物菌肥
Microbial fertilizer58.32 ± 1.18a 14.12 ± 0.15a 10.66 ±0.21b 7.50± 0.64b 0.37 ± 0.00b 20.37 ± 0.17b 表 3 不同处理对枇杷果实总酚、总黄酮、总黄烷醇的影响
Table 3. Effects of treatments on total phenols, flavonoids, and flavanols of loquat fruit
处理
Treatment总酚
Total phenols/(mg·hg−1)总黄酮
Total flavonoids/(mg·g−1)总黄烷醇
The total flavanol /(mg·hg−1)清水
Fresh water37.31 ± 0.50c 0.22 ± 0.00d 0.89 ± 0.03bc 氨基酸
Amino acid45.50 ± 0.48b 0.28 ± 0.00a 1.03 ± 0.03ab 磷酸二氢钾
Potassium dihydrogen phosphate44.25 ± 1.82b 0.26± 0.00b 1.02 ± 0.05ab 蔗糖
Sucrose43.42 ± 0.48b 0.26 ± 0.01c 0.78 ± 0.05c 钼酸铵
Ammonium molybdate38.69 ± 0.50c 0.22 ± 0.00d 1.17± 0.11a 微生物菌肥
Microbial fertilizer52.31 ± 0.61a 0.28± 0.00a 1.21 ± 0.67a -
[1] 刘丽丽, 刘玉垠, 王杰, 等. 枇杷功能成分及生物活性研究进展 [J]. 食品科学, 2020, 41(5):306−314. doi: 10.7506/spkx1002-6630-20190128-361LIU L L, LIU Y Y, WANG J, et al. A review of functional components and bioactivities in loquat (Eriobotrya japonica lindl. ) [J]. Food Science, 2020, 41(5): 306−314.(in Chinese) doi: 10.7506/spkx1002-6630-20190128-361 [2] ITO H, KOBAYASHI E, LI S H, et al. Antitumor activity of compounds isolated from leaves of Eriobotrya japonica [J]. Journal of Agricultural and Food Chemistry, 2002, 50(8): 2400−2403. doi: 10.1021/jf011083l [3] ITO H, KOBAYASHI E, LI S H, et al. Megastigmane glycosides and an acylated triterpenoid from Eriobotrya japonica [J]. Journal of Natural Products, 2001, 64(6): 737−740. doi: 10.1021/np010004x [4] 袁玉莹, 闫得朋, 尹航, 等. 叶面施肥对紫花苜蓿产草量和营养品质的影响及效益分析 [J]. 中国草地学报, 2020, 42(4):124−131.YUAN Y Y, YAN D P, YIN H, et al. Effects of foliar fertilizers on grass yield and nutritional quality of alfalfa and benefit analysis [J]. Chinese Journal of Grassland, 2020, 42(4): 124−131.(in Chinese) [5] 李萍, 单守明, 李映龙, 等. 叶面喷施氨基酸钙对盐碱地‘北玺’葡萄光合作用和果实品质的影响 [J]. 中国果树, 2021(12):22−26.LI P, SHAN S M, LI Y L, et al. Effects of calcium amino acid on photosynthesis and berry quality in ‘Beixi’ grapevine cultivate in saline-alkali soil [J]. China Fruits, 2021(12): 22−26.(in Chinese) [6] KARIMI R, KOULIVAND M, OLLAT N. Soluble sugars, phenolic acids and antioxidant capacity of grape berries as affected by iron and nitrogen [J]. Acta Physiologiae Plantarum, 2019, 41(7): 1−11. [7] 王武, 刘家红, 罗友进, 等. 喷施钙肥对贮藏期间W. 默科特果实品质的影响 [J]. 西南大学学报(自然科学版), 2014, 36(12):18−24.WANG W, LIU J H, LUO Y J, et al. Effects of foliar spray of calcium fertilizers on the postharvest quality of W. Murcott fruit [J]. Journal of Southwest University (Natural Science Edition), 2014, 36(12): 18−24.(in Chinese) [8] 王莹, 史振声, 王志斌, 等. 植物对氨基酸的吸收利用及氨基酸在农业中的应用 [J]. 中国土壤与肥料, 2008(1):6−11. doi: 10.3969/j.issn.1673-6257.2008.01.002WANG Y, SHI Z S, WANG Z B, et al. Absorption and utilization of amino acids by plant and application of amino acids on agiculture [J]. Soil and Fertilizer Sciences in China, 2008(1): 6−11.(in Chinese) doi: 10.3969/j.issn.1673-6257.2008.01.002 [9] 李翔, 刘忠珍, 魏岚, 等. 氨基酸微肥、钙镁肥和锌硼钼叶面肥对番石榴产量与品质的影响 [J]. 热带农业科学, 2021, 41(11):7−12.LI X, LIU Z Z, WEI L, et al. Zinc-boron-molybdenum foliar fertilizer on yield and quality of guava(Psidium guajava L. ) [J]. Chinese Journal of Tropical Agriculture, 2021, 41(11): 7−12.(in Chinese) [10] 齐红岩, 李天来, 陈元宏, 等. 叶面喷施磷酸二氢钾与葡萄糖对番茄光合速率和蔗糖代谢的影响 [J]. 农业工程学报, 2005, 21(S2):137−142.QI H Y, LI T L, CHEN Y H, et al. Effects of foliage applications of KH2PO4 and glucose on photosynthesis and sucrose metabolism of tomato [J]. Transactions of the Chinese Society of Agricultural Engineering, 2005, 21(S2): 137−142.(in Chinese) [11] 马雪强, 高子星, 王君正, 等. 钼酸铵对‘千玉6号’甜瓜果实营养品质和挥发性物质的影响 [J]. 西北农业学报, 2021, 30(11):1663−1673. doi: 10.7606/j.issn.1004-1389.2021.11.009MA X Q, GAO Z X, WANG J Z, et al. Effect of ammonium molybdate on nutritional quality and volatile substances of muskmelon‘Qianyu no. 6' [J]. Acta Agriculturae Boreali-Occidentalis Sinica, 2021, 30(11): 1663−1673.(in Chinese) doi: 10.7606/j.issn.1004-1389.2021.11.009 [12] 杨晓玉, 王艳萍, 冷平, 等. ABA对甜瓜果实成熟和软化的调节 [J]. 中国农业大学学报, 2010, 15(4):25−32. doi: 10.11841/j.issn.1007-4333.2010.04.005YANG X Y, WANG Y P, LENG P, et al. Effect of abscisic acid(ABA) on melon fruit ripening and softening [J]. Journal of China Agricultural University, 2010, 15(4): 25−32.(in Chinese) doi: 10.11841/j.issn.1007-4333.2010.04.005 [13] 李靖, 孙淑霞. 食品理化检验方法指南[M]. 北京: 北京大学出版社, 2011: . [14] 陈洁, 陈敏, 刘齐. 三种测定水果中维生素C含量方法的比较 [J]. 广东化工, 2017, 44(19):164−166. doi: 10.3969/j.issn.1007-1865.2017.19.077CHEN J, CHEN M, LIU Q. Comparison of three methods for the determination of vitamin C in fruits [J]. Guangdong Chemical Industry, 2017, 44(19): 164−166.(in Chinese) doi: 10.3969/j.issn.1007-1865.2017.19.077 [15] 陈伟, 施丽愉, 苏新国, 等. 不同品种枇杷果实采后低温贮藏品质研究 [J]. 食品安全质量检测学报, 2013, 4(6):1743−1749.CHEN W, SHI L Y, SU X G, et al. Research on postharvest quality in loquat fruit of different varieties during low temperature storage [J]. Journal of Food Safety & Quality, 2013, 4(6): 1743−1749.(in Chinese) [16] 杨璐, 努尔比耶, 李宏. 新疆红枣果实不同部位总酚的含量测定 [J]. 食品研究与开发, 2016, 37(1):152−156. doi: 10.3969/j.issn.1005-6521.2016.01.036YANG L, LUER B Y, LI H. Determination of total polyphenols in different parts of jujubae fruetus growing in Xinjiang [J]. Food Research and Development, 2016, 37(1): 152−156.(in Chinese) doi: 10.3969/j.issn.1005-6521.2016.01.036 [17] PARK Y S, JUNG S T, KANG S G, et al. Antioxidants and proteins in ethylene-treated kiwifruits [J]. Food Chemistry, 2008, 107(2): 640−648. [18] 熊庆娥. 植物生理学实验教程[M]. 成都: 四川科学技术出版社, 2003: 90-91. [19] BRAND-WILLIAMS W, CUVELIER M E, BERSET C. Use of a free radical method to evaluate antioxidant activity [J]. LWT - Food Science and Technology, 1995, 28(1): 25−30. doi: 10.1016/S0023-6438(95)80008-5 [20] BENZIE I F F, STRAIN J J. [2]Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration[M]//Oxidants and Antioxidants Part A. Amsterdam: Elsevier, 1999: 15-27. [21] 栗丽, 洪坚平, 谢英荷, 等. 生物菌肥对采煤塌陷复垦土壤生物活性及盆栽油菜产量和品质的影响 [J]. 中国生态农业学报, 2010, 18(5):939−944. doi: 10.3724/SP.J.1011.2010.00939LI L, HONG J P, XIE Y H, et al. Effect of bacterial manure on soil biological activity, yield and quality of rape in reclaimed core-mining areas [J]. Chinese Journal of Eco-Agriculture, 2010, 18(5): 939−944.(in Chinese) doi: 10.3724/SP.J.1011.2010.00939 [22] 张萍, 赵建华, 鞠召彬, 等. 放线菌肥对新疆加工番茄促生、防病增产及列当的防控效果 [J]. 中国蔬菜, 2019(2):49−52.ZHANG P, ZHAO J H, JU Z B, et al. Effect of actinomycete fertilizer on growth promotion, disease prevention, yield increase and broomrape control of processing tomato in Xinjiang [J]. China Vegetables, 2019(2): 49−52.(in Chinese) [23] 郭志刚, 李文芳, 马宗桓, 等. 生物菌肥和钾肥配施对苹果钾素吸收及果实品质的影响 [J]. 干旱地区农业研究, 2021, 39(3):113−121. doi: 10.7606/j.issn.1000-7601.2021.03.14GUO Z G, LI W F, MA Z H, et al. Effects of combined biological fertilizer and potassium fertilizer on potassium absorption and fruit quality of apple [J]. Agricultural Research in the Arid Areas, 2021, 39(3): 113−121.(in Chinese) doi: 10.7606/j.issn.1000-7601.2021.03.14 [24] 林建城, 林河通, 黄志明, 等. 福建省5个主栽品种枇杷果实品质比较及其与果实耐贮运的关系 [J]. 食品科学, 2008, 29(6):433−437. doi: 10.3321/j.issn:1002-6630.2008.06.097LIN J C, LIN H T, HUANG Z M, et al. Comparison of fruit qualities of five major loquat cultivars in Fujian Province and relationship between fruit quality and storability [J]. Food Science, 2008, 29(6): 433−437.(in Chinese) doi: 10.3321/j.issn:1002-6630.2008.06.097 [25] LI R, SUN S, WANG H J, et al. FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato [J]. Nature Communications, 2020, 11: 5844. doi: 10.1038/s41467-020-19705-w [26] 张建斌, 戚行江, 吴世军, 等. 氨基酸水溶肥对复壮杨梅树势和改善果实品质的作用 [J]. 湖南农业科学, 2021(5):27−29.ZHANG J B, QI X J, WU S J, et al. Effects of amino acid water-soluble fertilizer on tree vigor and fruit quality of waxberry (Myrica rubra) [J]. Hunan Agricultural Sciences, 2021(5): 27−29.(in Chinese) [27] 刘松忠, 刘军, 张强, 等. 不同肥料种类对黄金梨果实内在品质及风味的影响 [J]. 果树学报, 2012, 29(1):6−10.LIU S Z, LIU J, ZHANG Q, et al. Effects of manure types on sugars and acids content and flavor of pear(Pyrus pyrifolia cv. Hwangkumbae) [J]. Journal of Fruit Science, 2012, 29(1): 6−10.(in Chinese) [28] 许会会, 陈光, 王春夏, 等. 含氨基酸水溶肥对葡萄产量与质量及经济效益的影响 [J]. 现代农业科技, 2018(24):57,60.XU H H, CHEN G, WANG C X, et al. Effect of water-soluble fertilizer containing amino acids on grape's yield, quality and economic benefits [J]. Modern Agricultural Science and Technology, 2018(24): 57,60.(in Chinese) [29] 于雷, 邱菊, 赵丽影, 等. 氮素对玉米生长及生理特性的影响 [J]. 吉林农业科学, 2011, 36(2):36−39. doi: 10.3969/j.issn.1003-8701.2011.02.012YU L, QIU J, ZHAO L Y, et al. Effects of nitrogen on growth and physiological characteristics of maize [J]. Journal of Jilin Agricultural Sciences, 2011, 36(2): 36−39.(in Chinese) doi: 10.3969/j.issn.1003-8701.2011.02.012 [30] 王晓磊. 低钾下不同耐性玉米钾素积累及其延缓衰老的生理特性[D]. 沈阳: 沈阳农业大学, 2012.WANG X L. Physiological characteristics of potassium accumulation and delaying senescence of different maize (Zea mays L. ) under low potassium stress[D]. Shenyang: Shenyang Agricultural University, 2012. (in Chinese) [31] 唐岩, 宋来庆, 孙燕霞, 等. 叶面喷施磷酸二氢钾对红将军苹果叶片性状、果实品质和香气成分的影响 [J]. 山东农业科学, 2017, 49(5):82−85.TANG Y, SONG L Q, SUN Y X, et al. Effects of foliage application of potassium dihydrogen phosphate on blade, fruit quality and aroma components of red general fuji apple [J]. Shandong Agricultural Sciences, 2017, 49(5): 82−85.(in Chinese) [32] 谢国芳, 刘娜, 邓秋秋, 等. 采前喷施叶面肥对火龙果食用及贮藏品质的影响 [J]. 南方农业学报, 2019, 50(12):2755−2763.XIE G F, LIU N, DENG Q Q, et al. Effects of preharvest spraying with foliar fertilizer on edible quality and storage performance of pitaya [J]. Journal of Southern Agriculture, 2019, 50(12): 2755−2763.(in Chinese) [33] 刘志刚, 任红松, 买买提·艾合买提, 等. 氨基酸硒叶面肥对吐鲁番秋季露地甜瓜叶片早衰生理特性的影响 [J]. 新疆农业科学, 2021, 58(6):1078−1085.LIU Z G, REN H S, MAIMAITI AIHEMAITI, et al. Effects of amino acid selenium leaf fertilizer on the physiological characteristics of early senescence of autumn dew muskmelon leaves in Turpan [J]. Xinjiang Agricultural Sciences, 2021, 58(6): 1078−1085.(in Chinese)