硅对金线莲营养生长及其主要活性成分累积的影响

董倩; 王家旺; 黄国强

doi:10.19303/j.issn.1008-0384.2022.06.007

硅对金线莲营养生长及其主要活性成分累积的影响

doi: 10.19303/j.issn.1008-0384.2022.06.007

董倩^1,,
王家旺¹,
黄国强^2, ,

1.
福建技术师范学院食品与生物工程学院，福建　福清　350300
2.
福建农林大学，福建　福州　350002

基金项目: 福建省教育厅（A）类项目（JAT201370）

详细信息

作者简介:
董倩（1981−），女，硕士，讲师，研究方向：植物生理生化与分子生物学（E-mail：dqmeteor@163.com）

通讯作者:
黄国强（1975−），男，硕士，助理研究员，研究方向：作物遗传育种与营养栽培（E-mail：hgq94@163.com）

中图分类号: S 567.239
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- 被引次数: 0
出版历程
- 收稿日期: 2022-02-19
- 录用日期: 2022-02-19
- 修回日期: 2022-04-24
- 网络出版日期: 2022-05-21
- 刊出日期: 2022-06-28

Effects of Silicon on Growth and Functional Ingredients Accumulation of Anoectochilus roxburghii

1.
College of Food and Biological Engineering, Fujian Polytechnic Normal University, Fuqing, Fujian　350300, China
2.
Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China

摘要

摘要: 目的探究硅对金线莲营养生长及其主要活性成分累积的影响，为金线莲提质促产提供理论依据。方法以福建红霞金线莲为材料，采用水培的方式，施加不同浓度硅离子（0、0.175、0.350、0.525、0.700、0.875 mmol·L⁻¹）处理30 d，测定金线莲生长和相关生理生化指标。结果硅对金线莲生长有促进作用，0.175～0.700 mmol·L⁻¹处理后相对生长率均达对照的7倍以上；随着硅浓度的增加，叶绿素含量、叶绿素指数和氮平衡指数值均呈现明显的先升后降趋势，0.525 mmol·L⁻¹处理时均达到最高值（P＜0.01）；类黄酮指数则呈先降后升的趋势，在0.875 mmol·L⁻¹处理时达最高值（P＜0.01）；0.175 mmol·L⁻¹和0.700 mmol·L⁻¹的硅显著提高过氧化物酶的活性，为对照的2～3倍（P＜0.01）；0.700 mmol·L⁻¹的硅可显著促进金线莲总黄酮和多糖的累积（P＜0.01），含量较对照分别高71.45%和116.65%。结论硅对金线莲的生长有益，0.525 mmol·L⁻¹为营养生长期最佳浓度；提高浓度到0.700 mmol·L⁻¹有益于主要活性成分总黄酮和多糖的累积。
- 硅 /
- 金线莲 /
- 总黄酮 /
- 多糖 /
- 氮平衡指数
Abstract: Objective Effects of silicon on the growth and functional ingredients accumulation of Anoectochilus roxburghii were studied for the herbal crop quality improvement. Method Plants of Hong Xia, A. roxburghii (Wall.) Lindl, were supplied with concentrations of silicon at 0, 0.175, 0.350, 0.525, 0.700, and 0.875 mmol·L⁻¹ in a hydroponic experiment for 30 d. Effects of the treatments on the plant physiology and functional ingredients contents were monitored. Result Silicon addition promoted the growth of A. roxburghii. The relative growth rate under the supplementations of 0.175 to 0.700 mmol·L⁻¹ silicon was more than 7 times that of control. The chlorophyll content, chlorophyll index, and nitrogen balance index (NBI) all rose with increasing silicon to peak at 0.525 mmol·L⁻¹ and followed by a decline (P＜0.01), while the flavonoid decreased significantly to its lowest under 0.875 mmol·L⁻¹ (P＜0.01) and the peroxidase activity significantly increased more than 2 to 3 times that of control (P＜0.01). At high concentration of silicon (i.e., 0.700 mmol·L⁻¹), the accumulation of total flavonoids was 71.45%, and polysaccharides 116.65%, higher than those of control (P＜0.01). Conclusion Presence of silicon was beneficial for the growth of A. roxburghii. At an addition rate of 0.525 mmol·L⁻¹ during the vegetative growth period and 0.700 mmol·L⁻¹ in later stage, a maximized accumulation on flavonoids and polysaccharides could be realized.
- Silicon /
- Anoectochilus roxburghii (Wall.) Lindl. /
- flavonoids /
- polysaccharide /
- nitrogen balance index

HTML全文

图 1 不同浓度硅处理对金线莲相对生长率的影响

图中不同大、小写字母分别表示处理间的差异达显著水平P＜0.01或P＜0.05，下图同。

Figure 1. Effect of silicon at varied concentrations on relative growth rate of A. roxburghii

Data with different capital and lowercase letters mean significant difference at P＜0.01 and P＜0.05, respectively. Same for following figures.

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图 2 不同浓度硅处理对金线莲叶绿素含量的影响

Figure 2. Effect of silicon at varied concentrations on chlorophyll content of A. roxburghii

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图 3 金线莲叶片POD酶活性稳定性

Figure 3. Stability of peroxidase activity in A. roxburghii leaf

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图 4 葡萄糖标准曲线

Figure 4. Standard curve of glucose

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图 5 不同浓度硅处理对金线莲多糖含量的影响

Figure 5. Effect of silicon application at varied concentrations on polysaccharide content of A. roxburghii

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图 6 芦丁标准曲线

Figure 6. Standard curve of rutin (rutoside)

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图 7 硅对金线莲总黄酮含量的影响

Figure 7. Effect of silicon application at varied concentrations on flavonoids content of A. roxburghii

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表 1 不同浓度硅处理对金线莲氮平衡指数的影响

Table 1. Effect of silicon application at varied concentrations on NBI of A. roxburghii

硅Si/（mmol·L⁻¹）	叶绿素指数Chl	类黄酮指数Flav	花青素指数Anth	氮平衡指数NBI
0	29.0±1.2 Cc	0.22±0.04 BCb	0.08±0.01 a	133.4±25.1 CDcd
0.175	32.4±5.3 Bb	0.21±0.03 Cbc	0.08±0.01 a	158.0±49.9 BCbc
0.350	34.6±3.2 ABa	0.20±0.03 Cc	0.08±0.01 a	175.2±26.9 Bb
0.525	36.2±4.4 Aa	0.17±0.02 Dd	0.08±0.00 a	222.5±46.9 Aa
0.700	26.3±2.3 CDd	0.25±0.04 ABa	0.08±0.01 a	107.7±10.6 Ee
0.875	24.7±1.6 Dd	0.26±0.05 Aa	0.08±0.01 a	99.8±20.7 Ee
数据以平均值±标准偏差表示（n=3），同列不同大、小写字母分别表示处理间差异达极显著（P＜0.01）或显著水平（P＜0.05）。表2同。
Data are presented as mean±SD (n = 3); those with different capital and lowercase letters on same column mean significant difference at P＜0.01 and P＜0.05, respectively. Same for Table 2.

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表 2 不同浓度硅处理对金线莲不同叶位叶片POD酶活的影响

Table 2. Effect of silicon application at varied concentrations on peroxidase activity in differently located leaves on an A. roxburghii plant

硅Si/（mmol·L⁻¹）	+1 叶+1 leaf/（U·g⁻¹）	+2叶+2 leaf/（U·g⁻¹）	+3叶+3 leaf/（U·g⁻¹）
0	510±66 Bc	302±8 Cd	387±68 Bb
0.175	1425±268 Aa	1000±103 Aa	1157±212 Aa
0.350	688±33 Bbc	528±138 BCc	577±53 Bb
0.525	950±233 Bb	745±120 ABbc	622±63 Bb
0.700	1427±189 Aa	938±164 Aab	988±258 Aa
0.875	923±163 Bb	750±140 ABbc	628±25 Bb

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