不同基因型大豆苗期耐低磷性鉴定及指标筛选

王晶琴; 石贵阳; 杨松花; 陈竹; 杨通黎; 马秀国

doi:10.19303/j.issn.1008-0384.2022.06.003

不同基因型大豆苗期耐低磷性鉴定及指标筛选

doi: 10.19303/j.issn.1008-0384.2022.06.003

贵州大学农学院，贵州　贵阳　550025

基金项目: 国家自然科学基金项目（31860115）；贵州大学培育项目（202108）

详细信息

作者简介:
王晶琴（1996−），女，硕士研究生，研究方向：大豆耐低磷相关研究（E-mail：528117249@qq.com）

通讯作者:
陈竹（1982−），女，副教授，研究方向：大豆耐低磷相关研究（E-mail：274586492@qq.com）

中图分类号: S 565.1
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出版历程
- 收稿日期: 2022-01-20
- 修回日期: 2022-04-23
- 网络出版日期: 2022-08-07
- 刊出日期: 2022-06-28

Determination and Index Selection on Tolerance of Soybean Seedlings to Phosphorus Deficiency in Soil

College of Agriculture, Guizhou University, Guiyang, Guizhou　550025, China

摘要

摘要: 目的研究不同基因型大豆苗期的耐低磷特性，筛选耐低磷鉴定指标，为耐低磷大豆育种及其在西南地区的优化应用提供参考依据。方法以10个大豆品种为试验材料，设置低磷胁迫和正常供磷浓度2个处理，采用营养液水培法，测定株高、叶面积、地上部和地下部生物量、根长、根体积、根表面积、叶绿素含量、光合速率、蒸腾速率等19个形态指标及生理生化指标。以各项指标的耐低磷系数为耐低磷指标，通过因子分析、隶属函数、聚类分析和灰色关联度分析相结合的方法，综合评价大豆耐低磷性，并筛选评价指标。结果灰色关联度分析表明地上部鲜重、蒸腾速率、地上部干重、气孔导度、叶面积、株高、茎粗和根体积与大豆耐低磷性关系密切；主成分分析和隶属函数法计算大豆耐低磷能力D值，根据D值进行聚类分析，可将10个供试大豆品种按耐低磷强弱划分为3类：耐低磷型、中度耐低磷型和弱耐低磷型。结论苗期磷吸收和利用能力最强的大豆品种是铁丰31号、滇86-4。地上部鲜重、蒸腾速率、地上部干重、气孔导度、叶面积、株高、茎粗和根体积是评价大豆苗期耐低磷性的首选指标。
- 大豆 /
- 耐低磷 /
- 指标筛选 /
- 综合评价
Abstract: Objective Tolerance to deficient supply of phosphorus of soybean varieties at seedling stage was determined, and viable identification indicators selected to aid the breeding and resource utilization of soybean germplasms in southwest China. Method Ten soybean germplasms were treated by varying degrees of phosphorus stress along with a blank control in a hydroponic experiment. Nineteen morphological, physical, and biochemical properties on the seedlings, including plant height, leaf area, shoot and root biomass, root length, root volume, root surface area, chlorophyll content, photosynthetic rate, and transpiration rate, were monitored during the treatment for the index selection. The low-phosphorus tolerance coefficient on each indicator was then used to finalize the selection based on the analyses of factor, membership function, cluster, and grey correlation. Result The Grey correlation analysis showed that the shoot fresh weight, transpiration rate, shoot dry weight, stomatal conductance, leaf area, plant height, stem diameter, and root volume closely related to the tolerance of a soybean plant. The cluster analysis grouped the 10 soybean varieties into highly tolerant, moderately tolerant, and weakly tolerant categories. Conclusion Among the tested varieties Tiefeng No.31 and Dian 86-4 exhibited the strongest phosphorus absorption and utilization ability at seedling stage. The shoot fresh weight, transpiration rate, shoot dry weight, stomatal conductance, leaf area, plant height, stem diameter, and root volume were the choice indicators for evaluating the tolerance to deficient phosphorus supply of soybean plants at seedling stage.
- Soybean /
- low-phosphate tolerance /
- screening index /
- comprehensive evaluation

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图 1 不同大豆品种耐低磷能力的系统聚类分析

Figure 1. Dendrogram of soybean varieties based on low-phosphorus tolerance

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表 1 不同基因型大豆苗期各单项指标的耐低磷系数

Table 1. Low-phosphorus tolerance coefficients of soybean varieties at seedling stage

指标 Index	矮选 Aixuan	川豆14 Chuandou 14	滇86-4 Dian 86-4	汾豆62号 Fendou No.62	冀豆12号 Jidou No.12	晋豆23号 Jindou No.23	黔豆11号 Qiandou No.11	黔豆7号 Qiandou No.7	铁豆40号 Tiedou No.40	铁丰31号 Tiefeng No.31
X₁	0.757	1.038	0.999	0.809	0.872	0.745	0.983	0.862	0.963	1.088
X₂	0.829	0.938	0.992	0.885	1.121	0.908	1.197	0.994	0.974	1.016
X₃	0.793	0.927	0.980	0.939	1.148	0.834	0.762	0.824	0.849	0.964
X₄	0.633	0.810	0.990	0.547	0.893	0.564	0.834	0.844	0.869	0.936
X₅	0.721	0.869	0.969	0.746	0.931	0.793	0.842	0.836	0.904	1.030
X₆	0.995	1.057	1.350	1.430	1.480	1.085	0.910	1.326	1.387	1.254
X₇	1.104	1.224	1.597	1.570	1.475	1.613	0.836	1.076	1.432	1.452
X₈	0.790	0.788	1.210	1.362	1.482	1.166	0.825	1.060	0.952	1.218
X₉	1.080	1.017	1.376	0.869	0.925	0.941	1.025	1.019	1.160	1.446
X₁₀	0.853	1.176	2.192	0.515	0.593	0.594	0.940	0.808	1.514	1.447
X₁₁	1.291	1.307	1.139	0.658	0.623	0.743	1.243	1.015	1.197	1.194
X₁₂	1.237	0.486	0.648	1.741	1.597	1.289	0.343	1.399	1.135	0.353
X₁₃	1.553	1.394	1.636	2.097	1.627	2.059	0.971	1.241	1.579	1.353
X₁₄	1.003	0.786	1.096	0.904	0.879	1.127	1.097	0.980	0.607	0.628
X₁₅	1.001	0.931	0.903	0.983	0.938	1.153	1.008	1.084	1.070	1.053
X₁₆	0.750	0.564	0.439	0.766	0.984	1.787	0.904	0.719	0.932	0.828
X₁₇	0.746	0.652	0.587	0.756	0.866	1.081	0.826	0.692	0.649	0.667
X₁₈	0.988	0.808	0.940	0.457	1.391	1.386	1.043	0.832	1.331	1.199
X₁₉	1.018	1.021	0.977	1.180	1.416	1.151	1.100	0.965	1.355	1.143
X₁，株高；X₂，茎粗；X₃，叶面积；X₄，地上部鲜重；X₅，地上部干重；X₆，地下部鲜重；X₇，地下部干重；X₈，根长；X₉，根表面积；X₁₀，根体积；X₁₁，根平均直径；X₁₂，根尖数；X₁₃，根冠比；X₁₄，光合速率；X₁₅，胞间CO₂浓度；X₁₆，气孔导度；X₁₇，蒸腾速率；X₁₈，类胡萝卜素含量；X₁₉，叶绿素含量；下表同。 X₁: Plant height; X₂: Stem diameter; X₃: Leaf area; X₄: Shoot fresh weight; X₅: Shoot dry weight; X₆: Root fresh weight; X₇: Root dry weight; X₈: Root length; X₉: Root surface area; X₁₀: Root volume; X₁₁: Root average diameter; X₁₂: Root tips; X₁₃: Root shoot ratio; X₁₄: Photosynthetic rate; X₁₅: Intercellular CO₂ concentration; X₁₆: Stomatal conductance; X₁₇: Transpiration rate; X₁₈: Carotenoid content; X₁₉: Chlorophyll content. Same for the following tables.

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表 2 各单项指标耐低磷系数的相关系数矩阵

Table 2. Correlation coefficient matrix of low-phosphorus tolerance coefficients on individual indices

指标 Index	X₁	X₂	X₃	X₄	X₅	X₆	X₇	X₈	X₉	X₁₀	X₁₁	X₁₂	X₁₃	X₁₄	X₁₅	X₁₆	X₁₇	X₁₈	X₁₉
X₁	1
X₂	0.477	1
X₃	0.217	0.209	1
X₄	0.793^**	0.615	0.371	1
X₅	0.804^**	0.512	0.543	0.881^**	1
X₆	0.014	0.014	0.680^*	0.265	0.364	1
X₇	−0.101	−0.341	0.588	−0.082	0.273	0.631	1
X₈	−0.150	0.129	0.760^*	0.049	0.306	0.779^**	0.720^*	1
X₉	0.668^*	0.075	0.082	0.670^*	0.731^*	0.055	0.150	−0.051	1
X₁₀	0.693^*	0.079	0.087	0.710^*	0.664^*	0.106	0.178	−0.152	0.863^**	1
X₁₁	0.557	−0.002	−0.490	0.389	0.180	−0.561	−0.559	−0.833^**	0.565	0.588	1
X₁₂	−0.809^**	−0.367	0.190	−0.538	−0.523	0.534	0.331	0.489	−0.644^*	−0.619	−0.758^*	1
X₁₃	−0.583	−0.615	0.278	−0.612	−0.325	0.398	0.819^**	0.540	−0.309	−0.250	−0.677^*	0.641^*	1
X₁₄	−0.511	0.038	−0.281	−0.317	−0.464	−0.373	−0.176	−0.029	−0.321	−0.217	−0.211	0.121	0.098	1
X₁₅	−0.346	−0.162	−0.544	−0.360	−0.211	−0.161	−0.007	−0.089	−0.100	−0.325	−0.118	0.176	0.110	−0.023	1
X₁₆	−0.508	−0.029	−0.175	−0.494	−0.240	−0.172	0.253	0.180	−0.377	−0.508	−0.482	0.287	0.414	0.214	0.735^*	1
X₁₇	−0.633^*	0.058	−0.097	−0.587	−0.409	−0.259	0.106	0.217	−0.597	−0.704^*	−0.593	0.360	0.380	0.468	0.494	0.906^**	1
X₁₈	0.037	0.336	0.142	0.283	0.410	−0.019	0.168	0.098	0.195	0.077	−0.049	−0.092	−0.053	−0.146	0.374	0.595	0.417	1
X₁₉	−0.061	0.292	0.470	0.031	0.206	0.488	0.375	0.472	−0.236	−0.249	−0.492	0.378	0.285	−0.460	0.083	0.392	0.303	0.550	1
、分别表示在5%和1%水平差异显著。 and ** indicate significant differences at P＜5% and P＜1%, respectively.

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表 3 各综合指标主成分的特征向量及贡献率

Table 3. Eigenvectors and contribution rates of principal components of individual comprehensive index

指标 Index	因子载荷 Factor loading
指标 Index	CI₁	CI₂	CI₃	CI₄	CI₅
X₁	0.345	0.100	0.078	−0.017	−0.052
X₂	0.141	0.395	−0.172	−0.031	−0.166
X₃	0.013	0.387	−0.036	−0.095	0.203
X₄	0.318	0.347	−0.101	0.356	0.199
X₅	0.262	0.289	0.256	−0.085	−0.389
X₆	−0.041	0.130	0.514	0.162	0.019
X₇	−0.115	−0.112	0.321	0.355	−0.171
X₈	−0.135	0.088	0.354	−0.482	0.101
X₉	−0.154	−0.191	−0.026	−0.192	0.679
X₁₀	−0.685	0.181	0.126	−0.127	0.063
X₁₁	−0.780	0.118	−0.167	−0.080	−0.205
X₁₂	−0.720	0.095	−0.069	0.287	0.221
X₁₃	−0.361	−0.049	0.326	−0.053	0.223
X₁₄	0.770	−0.246	0.005	0.174	−0.125
X₁₅	0.803	0.098	0.030	0.354	0.179
X₁₆	0.779	0.175	−0.192	0.300	0.129
X₁₇	−0.815	0.288	0.187	0.051	0.103
X₁₈	−0.020	−0.008	0.402	0.199	0.095
X₁₉	−0.303	0.404	−0.077	−0.195	0.093
特征值 Eigenvalue	6.822	4.742	2.694	1.801	1.253
贡献率 Contributive ratio/%	35.905	24.960	14.181	9.479	6.592
累积贡献率 Cumulative contributive ratio/%	35.905	60.865	75.046	84.525	91.117

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表 4 各基因型大豆的综合指标值、权重、u( X_j)值及综合评价(D)

Table 4. Comprehensive index, index weight, u( X_j), and comprehensive evaluation value (D) of soybean cultivars

品种 Variety	CI₁	CI₂	CI₃	CI₄	CI₅	u( X₁)	u( X₂)	u( X₃)	u( X₄)	u( X₅)	D值 D value	位次 Rank
矮选 Aixuan	−0.892	−3.017	−1.120	0.575	−0.433	0.487	0.000	0.350	0.728	0.348	0.347	9
川豆14 Chuandou 14	2.083	−1.253	−1.053	−0.221	−0.376	0.880	0.261	0.364	0.517	0.361	0.431	7
滇86−4 Dian86−4	2.975	1.596	−1.622	0.554	2.303	0.998	0.683	0.245	0.722	1.000	0.766	2
汾豆62号 Fendou No.62	−3.383	0.944	−2.801	−0.436	−0.563	0.158	0.586	0.000	0.460	0.317	0.294	10
冀豆12号 Jidou No.12	−1.631	3.741	1.099	−2.174	−0.146	0.390	1.000	0.811	0.000	0.416	0.584	4
晋豆23号 Jindou No.23	−4.580	−0.645	1.970	1.603	1.119	0.000	0.351	0.992	1.000	0.718	0.407	8
黔豆11号 Qiandou No.11	1.570	−2.939	2.007	−1.906	0.510	0.812	0.012	1.000	0.071	0.572	0.528	5
黔豆7号 Qiandou No.7	−0.086	−1.206	−0.514	−0.757	−0.433	0.594	0.268	0.476	0.375	0.348	0.446	6
铁豆40号 Tiedou No.40	0.953	1.106	0.913	1.434	−1.891	0.731	0.610	0.772	0.955	0.000	0.675	3
铁丰31号 Tiefeng No.31	2.991	1.673	1.121	1.327	−0.090	1.000	0.694	0.816	0.927	0.429	0.839	1
权重 Weight						0.394	0.274	0.156	0.104	0.072

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表 5 各指标与耐低磷系数的关联度及位次

Table 5. Correlation and rank on low-phosphorus tolerance coefficients of individual indices

指标 Index	关联度 γ Correlation degree γ	权重 Weight	位次 Rank
株高 Plant height	0.716	0.057	6
茎粗 Stem diameter	0.682	0.054	7
叶面积 Leaf area	0.729	0.058	5
地上部鲜重 Shoot fresh weight	0.789	0.062	1
地上部干重 Shoot dry weight	0.742	0.059	3
地下部鲜重 Root fresh weight	0.580	0.046	17
地下部干重 Root dry weight	0.547	0.043	18
根长 Root length	0.644	0.051	13
根表面积 Root surface area	0.629	0.050	15
根体积 Root volume	0.678	0.054	8
根平均直径 Root average diameter	0.669	0.053	10
根尖数 Root tips	0.638	0.050	14
根冠比 Root shoot ratio	0.496	0.039	19
光合速率 Photosynthetic rate	0.649	0.051	12
胞间CO₂浓度 Intercellular CO₂ concentration	0.673	0.053	9
气孔导度 Stomatal conductance	0.736	0.058	4
蒸腾速率 Transpiration rat	0.775	0.061	2
类胡萝卜素含量 Carotenoid content	0.668	0.053	11
叶绿素含量 Chlorophyll content	0.619	0.049	16

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