内源激素对砂梨花芽休眠进程的调控作用

王会全; 李亮; 李永裕; 吴少华

doi:10.19303/j.issn.1008-0384.2022.012.008

内源激素对砂梨花芽休眠进程的调控作用

doi: 10.19303/j.issn.1008-0384.2022.012.008

王会全^{1, 2,},
李亮³,
李永裕¹,
吴少华^1, ,

1.
福建农林大学园艺植物天然产物研究所，福建　福州　350002
2.
福建农业职业技术学院，福建　福州　350303
3.
福建省农业科学院果树研究所，福建　福州　350013

基金项目: 高等学校博士学科点专项科研基金（20113515110011）；福建省科技计划重点项目（2012N005）；福建省科技计划引导性项目（2021N0020）

详细信息

作者简介:
王会全（1982−），男，博士，副教授，研究方向：果树生理及分子生物学（E-mail：476863105@qq.com）

通讯作者:
吴少华（1959−），男，硕士，教授，研究方向：果树生理及分子生物学（E-mail：wsh6677@hotmail.com）

中图分类号: S 661.2
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出版历程
- 收稿日期: 2022-08-14
- 修回日期: 2022-10-01
- 网络出版日期: 2022-12-28
- 刊出日期: 2022-03-28

Functions of Endogenous Hormones in Regulating Bud Dormancy of Pear Flowers

WANG Huiquan^{1, 2
,},
LI Liang³,
LI Yongyu¹,
WU Shaohua^{1
, ,}

1.
Institute of Natural Products of Horticultural Plants, Fujian Agriculture and Forestry University, Fuzhou, Fujian　350002, China
2.
Fujian Vocational College of Agriculture, Fuzhou, Fujian　350303, China
3.
Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian　350013, China

摘要

摘要: 目的分析砂梨花芽休眠进程中内源激素的含量及平衡关系对梨花芽休眠进程的调控作用，为研究南方短低温梨花芽休眠解除的调控机理奠定基础。方法以黄花梨和蜜雪梨2个砂梨品种花芽为试材，利用HPLC技术分析赤霉素（GA₃）、脱落酸（ABA）、3-吲哚乙酸（IAA）、玉米素（ZT）等4种内源激素含量以及GA₃/ABA、IAA/ABA、ZT/ABA、（IAA+GA₃）/ABA比值在2个砂梨品种花芽休眠进程中的变化。结果在砂梨花芽休眠进程中，GA₃和ABA的影响主要在内休眠解除启动期，并且GA₃促进了花芽内休眠解除启动，而ABA抑制了花芽休眠解除启动；IAA在休眠进程中的变化规律不明显，对于休眠解除的影响不显著；ZT在黄花梨中促进了内休眠的解除，而在蜜雪梨中则作用于内休眠解除的启动。GA₃/ABA和（IAA+GA₃）/ABA在砂梨花芽休眠进程中变化趋势基本相同，均表现出在内休眠阶段比值较小，并且变化趋势不明显，而内休眠解除启动时明显升高，并且在内休眠解除时稍低于内休眠解除启动期；IAA/ABA其对休眠进程的影响较小；ZT/ABA的比值在黄花梨花芽休眠解除启动期稍低于内休眠解除期，而在蜜雪梨中花芽休眠解除启动期会稍高于内休眠解除期。结论砂梨花芽休眠进程中GA₃和ABA两种內源激素起主要作用，ZT不能直接影响休眠进程，而且内源激素调控花芽的休眠进程更主要取决于各内源激素的平衡。不同品种之间內源激素变化不完全一致，但总的趋势相似，本研究为內源激素调控休眠的研究奠定了基础。
- 砂梨 /
- 内源激素 /
- GA₃ /
- ABA /
- 休眠
Abstract: Objective Functions of endogenous hormones in Pyrus pyrifolia on regulating the flower bud dormancy were studied. Method Flower buds of P. pyrifolia Huanghua and P. pyrifolia Mixue in various stages of dormancy were collected to determine the contents of GA₃, ABA, IAA, and ZT as well as the ratios of GA₃/ABA, IAA/ABA, ZT/ABA, and (IAA+GA₃)/ABA by HPLC. Result GA₃ and ABA exerted significant effects on the bud dormancy mostly during the endodormancy-releasing/bud-germinating stage as GA₃ initiated but ABA inhibited the release. IAA showed no significant effect in that regard, and ZT in Huanghua promoted the release, while ZT in Mixue initiated the dormancy. The ratios of GA₃/ABA and (IAA+GA₃)/ABA of both pear varieties were similarly low during dormancy. They rose significantly when the dormancy-release started and slightly declined afterward. The IAA/ABA ratio changed little, whereas the ZT/ABA decreased slightly in Huanghua, but the opposite in Mixue, at the initiation of dormancy-release. Conclusion GA₃ and ABA played a major role in the dormancy of the pear flower buds, but ZT did not directly affect the dormancy-release. The process appeared to be regulated by an integrated effect of balance among these endogenous hormones, rather than individually. Although the changes on the endogenous hormones during flower bud dormancy differed between the two pear varieties, a similar general trend seemed existed.
- Pyrus pyrifolia /
- endogenous hormones /
- GA₃ /
- ABA /
- dormancy

HTML全文

图 1 黄花梨花芽休眠进程中内源激素含量的变化

不同小写字母表示在不同采样日期间差异显著（P<0.05）；图2～3同。

Figure 1. Changes on endogenous hormones in dormancy of Huanghua flower buds

Different small letters show significant difference at the 0.05 probability levels in different sampling date. The same as Fig. 2-4.

下载: 全尺寸图片幻灯片

图 2 黄花梨花芽休眠进程中内源激素比值的变化

Figure 2. Changes on endogenous hormone ratios in dormancy of Huanghua flower buds

下载: 全尺寸图片幻灯片

图 3 蜜雪梨花芽休眠进程中内源激素含量的变化

Figure 3. Changes on endogenous hormones in dormancy of Mixue flower buds

下载: 全尺寸图片幻灯片

图 4 蜜雪梨花芽休眠进程中内源激素比值的变化

Figure 4. Changes on endogenous hormone ratios in dormancy of Mixue flower buds

下载: 全尺寸图片幻灯片

表 1 砂梨花芽采样时间及休眠进程

Table 1. Sampling time and dormancy process of P. pyrifolia flower buds

黄花梨 Pyrus pyrifolia. Huanghua			蜜雪梨 Pyrus pyrifolia. Mixue
编号 Number	采样时间 Sampling date/(年-月-日)	休眠进程 Dormancy process	编号 Number	采样时间 Sampling date/(年-月-日)	休眠进程 Dormancy process
H1	2013-12-05	未进入休眠 NO dormancy	M1	2013-11-29	未进入休眠 No dormancy
H2	2013-12-17	内休眠进入 Endodormancy starting	M2	2013-12-06	内休眠进入 Endodormancy starting
H3	2013-12-25	内休眠 Endodormancy	M3	2013-12-12	内休眠 Endodormancy
H4	2013-12-31	内休眠 Endodormancy	M4	2013-12-19	内休眠 Endodormancy
H5	2014-01-08	内休眠解除启动 Endodormancy release starting	M5	2013-12-26	内休眠 Endodormancy
H6	2014-01-15	内休眠解除 Endodormancy release	M6	2014-01-04	内休眠 Endodormancy
H7	2014-01-21	生态休眠 Ecodormancy	M7	2014-01-10	内休眠解除启动 Endodormancy release starting
H8	2014-01-27	生态休眠 Ecodormancy	M8	2014-01-16	内休眠解除 Endodormancy release
H9	2014-02-19	生态休眠 Ecodormancy	M9	2014-01-21	生态休眠 Ecodormancy
H10	2014-02-27	花芽萌动 Floral bud germinating	M10	2014-01-26	生态休眠 Ecodormancy
			M11	2014-02-07	花芽萌动 Floral bud germinating

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参考文献(20)

[1]	LIU G Q, LI W S, ZHENG P H, et al. Transcriptomic analysis of ‘Suli’ pear (Pyrus pyrifolia white pear group) buds during the dormancy by RNA-Seq [J]. BMC Genomics, 2012, 13: 700. doi: 10.1186/1471-2164-13-700
[2]	雷天, 吕招兰. 台农2号蜜雪梨“二次开花”的发生与克服措施 [J]. 果农之友, 2014(2):14,16. doi: 10.3969/j.issn.1671-7759.2014.02.008 LEI T, LYU Z L. The occurrence and overcoming measures of “second blossoming”of ‘Mixue’ pear [J]. Fruit Growers' Friend, 2014(2): 14,16.(in Chinese) doi: 10.3969/j.issn.1671-7759.2014.02.008
[3]	刘国琴. 梨休眠分子生理机制研究[D]. 杭州: 浙江大学, 2013. LIU G Q. Studies on molecular mechanism of pear dormancy[D]. Hangzhou: Zhejiang University, 2013. (in Chinese)
[4]	宋猜, 尹明宇, 姜仲茂, 等. ‘优一’杏花芽分化特性及其与休眠期内源激素含量变化的关系 [J]. 西北农林科技大学学报(自然科学版), 2017, 45(6):170−176,184. SONG C, YIN M Y, JIANG Z M, et al. Relationship between flower bud differentiation and change of endogenous hormone of ‘Youyi’ during dormancy period [J]. Journal of Northwest A & F University (Natural Science Edition), 2017, 45(6): 170−176,184.(in Chinese)
[5]	王海波, 高东升, 王孝娣, 等. 赤霉素和脱落酸与桃芽自然休眠诱导 [J]. 果树学报, 2006, 23(4):599−601. doi: 10.3969/j.issn.1009-9980.2006.04.024 WANG H B, GAO D S, WANG X D, et al. Role of gibberellin and abscisic acid in peach bud endodormancy induction [J]. Journal of Fruit Science, 2006, 23(4): 599−601.(in Chinese) doi: 10.3969/j.issn.1009-9980.2006.04.024
[6]	丛深, 王海波, 王孝娣, 等. 带叶休眠对休眠解除期间葡萄芽呼吸代谢的影响 [J]. 园艺学报, 2013, 40(10):1983−1989. doi: 10.3969/j.issn.0513-353X.2013.10.014 CONG S, WANG H B, WANG X D, et al. Effect of dormancy with leaves on respiration of grape buds during dormancy release [J]. Acta Horticulturae Sinica, 2013, 40(10): 1983−1989.(in Chinese) doi: 10.3969/j.issn.0513-353X.2013.10.014
[7]	孙利鑫, 张亚红. 落叶果树休眠期解除界定的研究进展 [J]. 中国果树, 2016(2):55−59. doi: 10.16626/j.cnki.issn1000-8047.2016.02.014 SUN L X, ZHANG Y H. Research progress on the definition of dormancy release of deciduous fruit trees [J]. China Fruits, 2016(2): 55−59.(in Chinese) doi: 10.16626/j.cnki.issn1000-8047.2016.02.014
[8]	郑国生, 盖树鹏, 盖伟玲. 低温解除牡丹芽休眠进程中内源激素的变化 [J]. 林业科学, 2009, 45(2):48−52. doi: 10.3321/j.issn:1001-7488.2009.02.009 ZHENG G S, GAI S P, GAI W L. Changes of endogenous hormones during dormancy release by chilling in tree peony [J]. Scientia Silvae Sinicae, 2009, 45(2): 48−52.(in Chinese) doi: 10.3321/j.issn:1001-7488.2009.02.009
[9]	马海燕. 葡萄生长过程中内源激素含量变化的研究[D]. 杨凌: 西北农林科技大学, 2007. MA H Y. Changes of endogenous hormones in grapevine during its development[D]. Yangling: Northwest A & F University, 2007. (in Chinese)
[10]	SAUTER A, ABRAMS S R, HARTUNG W. Structural requirements of abscisic acid (ABA) and its impact on water flow during radial transport of ABA analogues through maize roots [J]. Journal of Plant Growth Regulation, 2002, 21(1): 50−59. doi: 10.1007/s003440010040
[11]	毕磊. 七月酥梨休眠期内含物变化及生理代谢研究[D]. 保定: 河北农业大学, 2009. BI L. Study on changes of inclusions and metabolism during dormant period of qiyuesu pear[D]. Baoding: Hebei Agricultural University, 2009. (in Chinese)
[12]	袁燕波, 王历慧, 于晓南. 芍药休眠芽发育进程内源激素变化研究 [J]. 浙江农业学报, 2014, 26(1):54−60. doi: 10.3969/j.issn.1004-1524.2014.01.10 YUAN Y B, WANG L H, YU X N. Comparative analysis of endogenous hormones during dormant bud development of Chinese herbaceous peony [J]. Acta Agriculturae Zhejiangensis, 2014, 26(1): 54−60.(in Chinese) doi: 10.3969/j.issn.1004-1524.2014.01.10
[13]	高庆玉, 闫超, 张丙秀, 等. 树莓果实与休眠芽生长过程中内源激素含量变化研究 [J]. 东北农业大学学报, 2015, 46(1):19−25. doi: 10.3969/j.issn.1005-9369.2015.01.004 GAO Q Y, YAN C, ZHANG B X, et al. Study on the variation of endogenous hormone content during the growth of raspberry fruit and dormant bud [J]. Journal of Northeast Agricultural University, 2015, 46(1): 19−25.(in Chinese) doi: 10.3969/j.issn.1005-9369.2015.01.004
[14]	段成国, 李宪利, 高东升, 刘焕芳, 李萌. 剥鳞和激素处理对大樱桃花芽休眠解除及内源激素变化的影响 [J]. 西北植物学报, 2004, 24(4):615−620. doi: 10.3321/j.issn:1000-4025.2004.04.009 DUAN C G, LI X L, GAO D S, et al. Effects of removing scales and exogenous hormone treatments on changes of endogenous hormone in sweet cherry flower buds and dormancy release during dormancy [J]. Acta Botanica Boreali-Occidentalia Sinica, 2004, 24(4): 615−620.(in Chinese) doi: 10.3321/j.issn:1000-4025.2004.04.009
[15]	秦栋. 酸王(Malus domestica cv.‘Avrolles’)自然休眠解除后生理生化变化研究[D]. 泰安: 山东农业大学, 2009. QING D. Study on the physiological and biochemical changes in Avrolles(Malus domestica)after natural dormancy releasing[D]. Taian: Shandong Agricultural University, 2009. (in Chinese)
[16]	刘冬峰, 陈露露, 李国鹏, 等. 氰胺对‘翠冠’梨花芽休眠解除及内源激素和碳水化合物含量的影响 [J]. 浙江大学学报(农业与生命科学版), 2013, 39(1):69−74. LIU D F, CHEN L L, LI G P, et al. Effect of hydrogen cyanamide(HC) on release of floral bud dormancy, endogenous hormone and carbohydrate in Pyrus pyrifolia cv. Cuiguan [J]. Journal of Zhejiang University (Agriculture and Life Sciences), 2013, 39(1): 69−74.(in Chinese)
[17]	WANG H Q, LIN Z Y, CHEN J N, et al. Classification of dormancy stage in Asian pear buds under the influence of, natural environment [J]. Fresenius Environmental Bulletin, 2020, 29(2): 1241−1249.
[18]	赵娟娟. 柚果实生长过程中内源激素含量变化的研究[D]. 福州: 福建农林大学, 2013 ZHAO J J. Study on the changes of endogenous hormone contents in pomelo fruit growth process[D]. Fuzhou: Fujian Agriculture and Forestry University, 2013. (in Chinese)
[19]	牛庆丰. 梨花芽休眠转换的分子网络及调控机制[D]. 杭州: 浙江大学, 2016. NIU Q F. The molecular mechanism and molecular network of pear flower bud dormancy transition[D]. Hangzhou: Zhejiang University, 2016. (in Chinese)
[20]	SEELEY S D, DAMAVANDY H, ANDERSON J L, et al. Autumn-applied growth regulators influence leaf retention, bud hardiness, bud and flower size, and endodormancy in peach and cherry [J]. Journal of the American Society for Horticultural Science, 1992, 117(2): 203−208. doi: 10.21273/JASHS.117.2.203